目的:研究Ras蛋白激活物类似物-1基因(Ras protein activator like-1,RASAL1)在正常胃黏膜上皮细胞、胃癌细胞株中的表达情况及其生物学意义。方法:采用RT-PCR和Western blot分别检测高分化胃腺癌细胞株MKN-28、中分化胃腺癌细胞株SGC-7...目的:研究Ras蛋白激活物类似物-1基因(Ras protein activator like-1,RASAL1)在正常胃黏膜上皮细胞、胃癌细胞株中的表达情况及其生物学意义。方法:采用RT-PCR和Western blot分别检测高分化胃腺癌细胞株MKN-28、中分化胃腺癌细胞株SGC-7901、低分化胃腺癌细胞株BGC-823和正常胃黏膜上皮细胞株GES-1中RASAL1基因在mRNA水平和蛋白水平的表达,应用基因测序法检测上述3种胃癌细胞株中K-Ras基因第一外显子第12密码子点突变情况,并分析其意义。结果:RT-PCR和Westernblot检测结果显示,与正常胃黏膜上皮细胞相比,在3种不同分化胃腺癌细胞株中,RASAL1基因的表达在mRNA水平和蛋白水平均下调(P<0.05),分化程度越低表达下调越明显;测序结果表明3种胃腺癌细胞株中均未发现K-Ras基因第一外显子第12密码子点突变。结论:RASAL1基因在含野生型K-Ras的胃癌细胞中呈低表达状态,其表达水平与胃癌的恶性程度有一定的关系。展开更多
The treatment of wheat （ Triticum aestivum L.） seedlings with CaCl 2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca 2+ chelator EGTA decreased the expression of the hsp26 gene. The expressi...The treatment of wheat （ Triticum aestivum L.） seedlings with CaCl 2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca 2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM12 is rapidly upregulated in wheat seedlings by heat shock at 37 ℃. The heatinduced upregulation of CaM12 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N6aminohexyl5chloro1naphthalenesulfonamid （W7） inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calciumcalmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.展开更多
文摘目的:研究Ras蛋白激活物类似物-1基因(Ras protein activator like-1,RASAL1)在正常胃黏膜上皮细胞、胃癌细胞株中的表达情况及其生物学意义。方法:采用RT-PCR和Western blot分别检测高分化胃腺癌细胞株MKN-28、中分化胃腺癌细胞株SGC-7901、低分化胃腺癌细胞株BGC-823和正常胃黏膜上皮细胞株GES-1中RASAL1基因在mRNA水平和蛋白水平的表达,应用基因测序法检测上述3种胃癌细胞株中K-Ras基因第一外显子第12密码子点突变情况,并分析其意义。结果:RT-PCR和Westernblot检测结果显示,与正常胃黏膜上皮细胞相比,在3种不同分化胃腺癌细胞株中,RASAL1基因的表达在mRNA水平和蛋白水平均下调(P<0.05),分化程度越低表达下调越明显;测序结果表明3种胃腺癌细胞株中均未发现K-Ras基因第一外显子第12密码子点突变。结论:RASAL1基因在含野生型K-Ras的胃癌细胞中呈低表达状态,其表达水平与胃癌的恶性程度有一定的关系。
文摘The treatment of wheat （ Triticum aestivum L.） seedlings with CaCl 2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca 2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM12 is rapidly upregulated in wheat seedlings by heat shock at 37 ℃. The heatinduced upregulation of CaM12 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N6aminohexyl5chloro1naphthalenesulfonamid （W7） inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calciumcalmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.
