目的:为了筛选减少烤烟生产中青枯病和黑胫病危害的生物拮抗菌剂,方法:以市售生物菌剂和新研发的腺毛二萜提取物为材料,开展盆栽实验研究其对烤烟青枯病、黑胫病防效及对烤烟农艺性状影响,开展田间试验研究其对烤烟经济性状、化学成分...目的:为了筛选减少烤烟生产中青枯病和黑胫病危害的生物拮抗菌剂,方法:以市售生物菌剂和新研发的腺毛二萜提取物为材料,开展盆栽实验研究其对烤烟青枯病、黑胫病防效及对烤烟农艺性状影响,开展田间试验研究其对烤烟经济性状、化学成分的影响,结果:结果显示,稼碧乐生物菌剂对青枯病、黑胫病有较强的直接抑制作用;菌为农生物菌剂、稼碧乐生物菌剂、阵地-5亿/mL多粘类芽孢杆菌KN-03液体和烟草腺毛二萜提取物10%微乳剂对烤烟青枯病和黑胫病的发生均有防效,3种菌剂和诱抗剂均表现出促进生长发育的作用;稼碧乐生物菌剂和烟草腺毛二萜提取物10%微乳剂对增加烤烟经济性状有较明显的正向作用,施用处理所产烟叶的化学成分指标较优。结论:在云南省文山州烤烟生产种,稼碧乐生物菌剂和烟草腺毛二萜提取物10%微乳剂是防效较优的2种防治烤烟青枯病、黑胫病的生物药剂。Purpose: In order to screen biological antagonistic agents that reduce the harm of tobacco wilt and black shank disease in tobacco production, Method: Using commercially available biological agents and glandular hair diterpenoid extracts as materials, pot experiments were conducted to study their control effects on tobacco wilt and black shank disease, as well as their impact on the agricultural traits of tobacco. Field experiments were also conducted to study their effects on the economic traits and chemical composition of tobacco. Results: The results showed that Jiabile biological microbial agent has a strong direct inhibitory effect on bacterial wilt and black shank disease;the three bacterial agents, namely Nongjiao and Jiabile biological agents, Bacillus subtilis KN-03 liquid at a concentration of -500 million/mL, and 10% microemulsion of tobacco glandular hair diterpenoid extract, were effective in preventing the occurrence of tobacco wilt and black shank disease. All three bacterial agents and inducers showed efficacy. Promote growth and development;Jiabile bio microbial agent and 10% microemulsion of tobacco glandular diterpenoid extract have a significant positive effect on increasing the economic traits of tobacco leaves, and the chemical composition indicators of the tobacco leaves produced by the application treatment are better. Conclusion: In the production of tobacco in Wenshan Prefecture, Yunnan Province, Jiabile bio microbial agent and 10% microemulsion of tobacco glandular diterpenoid extract are the two most effective pesticides for controlling tobacco wilt and black shank disease.展开更多
通过筛选籼稻恢复系明恢86的组培变异后代,获得1个类病斑及早衰突变体lms1(lesion mimic and senescence 1)。lms1植株生长至拔节期开始在叶片上出现黄褐色小斑点,随后斑点逐渐扩展至大部分叶片和茎组织;生长至抽穗期后呈现早衰,穗、茎...通过筛选籼稻恢复系明恢86的组培变异后代,获得1个类病斑及早衰突变体lms1(lesion mimic and senescence 1)。lms1植株生长至拔节期开始在叶片上出现黄褐色小斑点,随后斑点逐渐扩展至大部分叶片和茎组织;生长至抽穗期后呈现早衰,穗、茎、叶明显干枯,并快速衰亡。RT-PCR分析表明,在呈现类病斑性状叶片组织的lsm1中,病程相关基因PBZ1、PAL1表达明显高于其在野生型叶片组织中的水平。遗传分析表明,lms1的突变性状受单隐性核基因控制。利用9311与lms1配置的F2及F3群体进行基因定位,将lms1基因定位在水稻第11染色体长臂末端。展开更多
文摘目的:为了筛选减少烤烟生产中青枯病和黑胫病危害的生物拮抗菌剂,方法:以市售生物菌剂和新研发的腺毛二萜提取物为材料,开展盆栽实验研究其对烤烟青枯病、黑胫病防效及对烤烟农艺性状影响,开展田间试验研究其对烤烟经济性状、化学成分的影响,结果:结果显示,稼碧乐生物菌剂对青枯病、黑胫病有较强的直接抑制作用;菌为农生物菌剂、稼碧乐生物菌剂、阵地-5亿/mL多粘类芽孢杆菌KN-03液体和烟草腺毛二萜提取物10%微乳剂对烤烟青枯病和黑胫病的发生均有防效,3种菌剂和诱抗剂均表现出促进生长发育的作用;稼碧乐生物菌剂和烟草腺毛二萜提取物10%微乳剂对增加烤烟经济性状有较明显的正向作用,施用处理所产烟叶的化学成分指标较优。结论:在云南省文山州烤烟生产种,稼碧乐生物菌剂和烟草腺毛二萜提取物10%微乳剂是防效较优的2种防治烤烟青枯病、黑胫病的生物药剂。Purpose: In order to screen biological antagonistic agents that reduce the harm of tobacco wilt and black shank disease in tobacco production, Method: Using commercially available biological agents and glandular hair diterpenoid extracts as materials, pot experiments were conducted to study their control effects on tobacco wilt and black shank disease, as well as their impact on the agricultural traits of tobacco. Field experiments were also conducted to study their effects on the economic traits and chemical composition of tobacco. Results: The results showed that Jiabile biological microbial agent has a strong direct inhibitory effect on bacterial wilt and black shank disease;the three bacterial agents, namely Nongjiao and Jiabile biological agents, Bacillus subtilis KN-03 liquid at a concentration of -500 million/mL, and 10% microemulsion of tobacco glandular hair diterpenoid extract, were effective in preventing the occurrence of tobacco wilt and black shank disease. All three bacterial agents and inducers showed efficacy. Promote growth and development;Jiabile bio microbial agent and 10% microemulsion of tobacco glandular diterpenoid extract have a significant positive effect on increasing the economic traits of tobacco leaves, and the chemical composition indicators of the tobacco leaves produced by the application treatment are better. Conclusion: In the production of tobacco in Wenshan Prefecture, Yunnan Province, Jiabile bio microbial agent and 10% microemulsion of tobacco glandular diterpenoid extract are the two most effective pesticides for controlling tobacco wilt and black shank disease.
文摘通过筛选籼稻恢复系明恢86的组培变异后代,获得1个类病斑及早衰突变体lms1(lesion mimic and senescence 1)。lms1植株生长至拔节期开始在叶片上出现黄褐色小斑点,随后斑点逐渐扩展至大部分叶片和茎组织;生长至抽穗期后呈现早衰,穗、茎、叶明显干枯,并快速衰亡。RT-PCR分析表明,在呈现类病斑性状叶片组织的lsm1中,病程相关基因PBZ1、PAL1表达明显高于其在野生型叶片组织中的水平。遗传分析表明,lms1的突变性状受单隐性核基因控制。利用9311与lms1配置的F2及F3群体进行基因定位,将lms1基因定位在水稻第11染色体长臂末端。
