The Cajanus cajan L.is a natural plant,which contains lots of potential active components.The effects of the stilbene extracts from Cajanus cajan L.（sECC） on ovariectomy（OVX）-induced bone loss in rats were identif...The Cajanus cajan L.is a natural plant,which contains lots of potential active components.The effects of the stilbene extracts from Cajanus cajan L.（sECC） on ovariectomy（OVX）-induced bone loss in rats were identified.All experimental female rats were divided into 6 groups,i.e.,sham-operated rats,OVX rats,17β-estradiol（E2）-treated rats,sECC-treated rats with three dosages,50,100,and 200(mg·kg-1),separately.Two weeks after the operation,different dosage of sECC,E2 or deionized water were given to the 6 groups of rats,respectively for another 8 weeks through stomach.Then,all rats were killed.The body weight and uterus wet weight were measured.Contents of serum E2,follicle stimulating hormone（FSH）,and luteinizing hormone（LH） were measured by radioimmunoassay.Femoral morphology was observed by HE stain.The results showed that there were no changes of the uterine weight and serum E2 concentration in sECC-treated rats compared with OVX rats.However,the serum FSH and LH concentrations reduced by 11.5% and 15.2%（P<0.05）,respectively.By HE staining,it is found that the 60% of the femur structure had been significantly improved in OVX rats treated with 200 mg·kg-1 of sECC.The trabeculae were thicker and larger than that of OVX rats.It is clear that sECC improved femoral morphological structure and decreased FSH and LH contents without affecting serum E2 level and uterine weight in OVX rats.The results suggested that sECC had potential action in treatment of postmenopausal osteoporosis.展开更多
试验优选了过山枫(Celastrus aculeatus Merr.)总芪类的提取和纯化工艺条件。以过山枫总芪类提取率为指标,通过正交试验优选提取总芪类的工艺条件;以大孔树脂的比饱和吸附量与总芪类的回收率为指标,通过静态和动态试验优选树脂纯化总芪...试验优选了过山枫(Celastrus aculeatus Merr.)总芪类的提取和纯化工艺条件。以过山枫总芪类提取率为指标,通过正交试验优选提取总芪类的工艺条件;以大孔树脂的比饱和吸附量与总芪类的回收率为指标,通过静态和动态试验优选树脂纯化总芪类的工艺条件。结果表明,最佳提取工艺条件为5倍量95%乙醇加热回流提取1次,每次1 h,总芪类提取率为0.74%。最佳树脂纯化工艺条件为选择DM130树脂,采用20 mm直径的层析柱,上样溶液的总芪类浓度为5.86 mg/m L,10 g树脂上样30 m L,流速控制在3 m L/min,待吸附后先以30%乙醇洗脱200 m L除杂,再用200 m L 50%乙醇解吸。纯化后总芪类的含量达到58.52%,回收率为60.92%。此优选的工艺合理可行,能够较好地从过山枫中提取和纯化总芪类。展开更多
文摘The Cajanus cajan L.is a natural plant,which contains lots of potential active components.The effects of the stilbene extracts from Cajanus cajan L.（sECC） on ovariectomy（OVX）-induced bone loss in rats were identified.All experimental female rats were divided into 6 groups,i.e.,sham-operated rats,OVX rats,17β-estradiol（E2）-treated rats,sECC-treated rats with three dosages,50,100,and 200(mg·kg-1),separately.Two weeks after the operation,different dosage of sECC,E2 or deionized water were given to the 6 groups of rats,respectively for another 8 weeks through stomach.Then,all rats were killed.The body weight and uterus wet weight were measured.Contents of serum E2,follicle stimulating hormone（FSH）,and luteinizing hormone（LH） were measured by radioimmunoassay.Femoral morphology was observed by HE stain.The results showed that there were no changes of the uterine weight and serum E2 concentration in sECC-treated rats compared with OVX rats.However,the serum FSH and LH concentrations reduced by 11.5% and 15.2%（P<0.05）,respectively.By HE staining,it is found that the 60% of the femur structure had been significantly improved in OVX rats treated with 200 mg·kg-1 of sECC.The trabeculae were thicker and larger than that of OVX rats.It is clear that sECC improved femoral morphological structure and decreased FSH and LH contents without affecting serum E2 level and uterine weight in OVX rats.The results suggested that sECC had potential action in treatment of postmenopausal osteoporosis.
文摘试验优选了过山枫(Celastrus aculeatus Merr.)总芪类的提取和纯化工艺条件。以过山枫总芪类提取率为指标,通过正交试验优选提取总芪类的工艺条件;以大孔树脂的比饱和吸附量与总芪类的回收率为指标,通过静态和动态试验优选树脂纯化总芪类的工艺条件。结果表明,最佳提取工艺条件为5倍量95%乙醇加热回流提取1次,每次1 h,总芪类提取率为0.74%。最佳树脂纯化工艺条件为选择DM130树脂,采用20 mm直径的层析柱,上样溶液的总芪类浓度为5.86 mg/m L,10 g树脂上样30 m L,流速控制在3 m L/min,待吸附后先以30%乙醇洗脱200 m L除杂,再用200 m L 50%乙醇解吸。纯化后总芪类的含量达到58.52%,回收率为60.92%。此优选的工艺合理可行,能够较好地从过山枫中提取和纯化总芪类。
