Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxid...Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxide composite matrix was prepared through the method of water-in-oil suspension thermal regeneration. Its typical physical properties were wet density 1.18g.cm-3, diameters in the range of 100-300um, porosity 85.5%, and water content 72.3%. Expansion characteristics and liquid mixing performance of the matrix in expanded bed were investigated using water and 10% (by mass) glycerol solution as mobile phases. The results indicate that the custom-assembled matrix has a stable flow hydrodynamics and exhibits the same degree of liquid-phase mixing or column efficiency as the commercially available Streamline adsorbent.展开更多
This study was carried out during 2007-2009 in the vineyards of Ardebil province of lran to study the biology and the epidemiology of Uncinula necator--the causal agent of grape powdery mildew disease. The study conce...This study was carried out during 2007-2009 in the vineyards of Ardebil province of lran to study the biology and the epidemiology of Uncinula necator--the causal agent of grape powdery mildew disease. The study concentrated on the survival and the initiation of primary inoculum of the fungal causal agent. Results of histopathological experiments indicated that U. necator survived as mycelium in the dormant buds of the grapes during winter season. Results of study on the effect of environmental factors on fungus biology showed that the pathogenic activity of the fungus began when the temperature was between 16-19 ℃ with a relative humidity more than 50%. It was also found that optimum temperature and relative humidity for the sporulation of U.necator was 20-25 ~C and 50%-100% respectively. According to the results, fungal conidia were trapped during formation of 5-6 true leaves and first disease symptoms were observed on the clusters on late June after fruit formation. Fungal cleistothecia were observed abundantly at the end of season on the leaves, petioles and twigs but they were not able to survive during winter. Formation of ascospores on young leaves was proved but their role as the primary inoculum was not supported by the results of this study. Results of this study and the new findings on the biology and epidemiology of U.necator may be of national and international interests for the management of powdery mildew disease which is one of the most destructive diseases around the world including Iran.展开更多
基金Supported by the National Natural Science Foundation of China(No.20076042,No.20206029)and the Scientific Research Foundation of the State Education Ministry for the Returned Overseas Chinese Scholars(No.2002-247).
文摘Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxide composite matrix was prepared through the method of water-in-oil suspension thermal regeneration. Its typical physical properties were wet density 1.18g.cm-3, diameters in the range of 100-300um, porosity 85.5%, and water content 72.3%. Expansion characteristics and liquid mixing performance of the matrix in expanded bed were investigated using water and 10% (by mass) glycerol solution as mobile phases. The results indicate that the custom-assembled matrix has a stable flow hydrodynamics and exhibits the same degree of liquid-phase mixing or column efficiency as the commercially available Streamline adsorbent.
文摘This study was carried out during 2007-2009 in the vineyards of Ardebil province of lran to study the biology and the epidemiology of Uncinula necator--the causal agent of grape powdery mildew disease. The study concentrated on the survival and the initiation of primary inoculum of the fungal causal agent. Results of histopathological experiments indicated that U. necator survived as mycelium in the dormant buds of the grapes during winter season. Results of study on the effect of environmental factors on fungus biology showed that the pathogenic activity of the fungus began when the temperature was between 16-19 ℃ with a relative humidity more than 50%. It was also found that optimum temperature and relative humidity for the sporulation of U.necator was 20-25 ~C and 50%-100% respectively. According to the results, fungal conidia were trapped during formation of 5-6 true leaves and first disease symptoms were observed on the clusters on late June after fruit formation. Fungal cleistothecia were observed abundantly at the end of season on the leaves, petioles and twigs but they were not able to survive during winter. Formation of ascospores on young leaves was proved but their role as the primary inoculum was not supported by the results of this study. Results of this study and the new findings on the biology and epidemiology of U.necator may be of national and international interests for the management of powdery mildew disease which is one of the most destructive diseases around the world including Iran.
