Variation of two simple sequence repeats (SSRs) in the leader region of the waxy gene was analyzed in a sample of 74 accessions, including Oryza sativa L. ssp. indica, japonica and wild rice (O.rufipogon) represen...Variation of two simple sequence repeats (SSRs) in the leader region of the waxy gene was analyzed in a sample of 74 accessions, including Oryza sativa L. ssp. indica, japonica and wild rice (O.rufipogon) representing a wide distribution range of amylose content (AC) in cultivated rice. Eight alleles were detected in the (CT) n motif and two alleles were resolved in the (AATT) n motif. The distribution of the alleles of the two SSRs was quite uneven as detected by the (CT) n motif. The repeat numbers of the two SSR motifs, (CT) n and (AATT) n, appeared to be inversely related such that the total length of this region was maintained. AC of the varieties was highly correlated with the length of SSRs. Differences in AC among the various SSR genotypes were statistically highly significant as analyzed using genotypes of both SSR motifs. Although the SSR variation did not seem to have obvious function in the synthesis of the starch synthase encoded by the waxy gene, the almost perfect correlation between the two SSRs and AC level could be used for quality improvement in rice breeding programs.展开更多
Sweetpotato starch thermal properties and its noodle quality were analyzed using a rapid predictive method based on near-infrared spectroscopy (NIRS). This method was established based on a total of 93 sweetpotato g...Sweetpotato starch thermal properties and its noodle quality were analyzed using a rapid predictive method based on near-infrared spectroscopy (NIRS). This method was established based on a total of 93 sweetpotato genotypes with diverse genetic background. Starch samples were scanned by NIRS and analyzed for quality properties by reference methods. Results of statistical modelling indicated that NIRS was reasonably accurate in predicting gelatinization onset temperature (To) (standard error of prediction SEP=2.014 ℃, coefficient of determination RSQ=0.85), gelatinization peak temperature (Tp) (SEP=-1.371 ℃, RSQ=0.89), gelatinization temperature range (Tr) (SEP=2.234 ℃, RSQ=0.86), and cooling resistance (CR) (SEP=0.528, RSQ=0.89). Gelatinization completion temperature (To), enthalpy of gelatinization (△H), cooling loss (CL) and swelling degree (SWD), were modelled less well with RSQ between 0.63 and 0.84. The present results suggested that the NIRS based method was sufficiently accurate and practical for routine analysis of sweetpotato starch and its noodle quality.展开更多
This work is focused on the characterization and rapid analytical determination of cereal flour quality with regard to nutritional and breadmaking quality. Starch, protein and non-starch polysaccharides are the main c...This work is focused on the characterization and rapid analytical determination of cereal flour quality with regard to nutritional and breadmaking quality. Starch, protein and non-starch polysaccharides are the main components of cereals. The content and quality of proteins and content of damaged starch is important because of the technological quality of flours. The high content of high molecular weight proteins is substantial for bread technology especially, while soluble protein fractions and non-starch polysaccharides are important for nutrition. The set of wheat, barley and rye flours and their blends were analyzed and their properties and their qualitative parameters were determined. Principal component analysis (PCA) was used on Fourier transform-infrared (FT-IR) spectra in the 1,200-800 cm1 wavenumber region and significant correlations of various nutritional and breadmaking parameters were observed. Results showed that the FT-IR spectroscopy and PCA can serve for rapid screening and classification of cereal flour quality.展开更多
The effect of varied processing variables (yam slice thickness, drying temperatures and type of drying) were investigated to determine their effects on the proximate and some functional properties of yam flour. The ...The effect of varied processing variables (yam slice thickness, drying temperatures and type of drying) were investigated to determine their effects on the proximate and some functional properties of yam flour. The yam flour was produced with different slice thickness of 1, 1.5, 2.0, 2.5 and 5 cm using both conventional sun drying method and oven drying at temperatures; 40 ~C, 50 ~C, 60 ~C and 70 ~C. The moisture content of all the yam flour samples increased with increasing yam slice thickness, with the samples sun dried having the lowest values (6.20%-6.87%) followed by those dried at 70 ~C and then 60 ~C. The protein content of the yam flour samples increased with increase in slice thickness and decreased with increase in drying temperatures while the fat and the crude fibre of all the yam flour samples decreased with increase in slice thickness. The water absorption capacity of the sun dried yam flour samples decreased with increase in slice thickness while the syneresis value, bulk density and the gel strength increased with increase in slice thickness for all drying temperatures and for sun dried yam flour samples but smaller slice thickness had higher swelling capacity.展开更多
Aloe gel, derived from Aloe vera plant is well known for its nutraceutical potential, It is available commercially as a juice with poor sensory quality. The therapeutic benefits of Aloe gel could be extended to food p...Aloe gel, derived from Aloe vera plant is well known for its nutraceutical potential, It is available commercially as a juice with poor sensory quality. The therapeutic benefits of Aloe gel could be extended to food products concomitantly improving its acceptability. Dahi/curd is an Asian fermented dairy product that enjoys tremendous mass appeal. The objective of the present study was to formulate dahi enriched with Aloe gel (AG) as a functional ingredient and to study its effect on the quality of dahi. Plain dahi was prepared by addition of 2.5% skimmed milk powder (SMP) and suitable starter to milk. For the preparation of AG enriched dahi, SMP was replaced by AG at different levels (0.1-0.25%). The products were evaluated for moisture, pH, titrable acidity (TA), whey syneresis (WS), total solid content (TS), water holding capacity (WHC), total yield (TY), whiteness index (WI), and viscosity. Microbial analysis and sensory evaluation were also carried out. Enrichment of dahi with AG was found to improve its quality characteristics by causing a significant reduction in WS and an increase in TA, TS, WHC, TY, WI and viscosity. AG dahi recorded good sensory acceptability. Storage study also indicated AG dahi to perform better in terms of the various parameters studied. The present investigation suggests the feasibility of adding Aloe gel powder as a functional ingredient to dahi to enhance product quality as well as its nutritional and therapeutic potency. It could be commercially exploited as a novel product.展开更多
文摘Variation of two simple sequence repeats (SSRs) in the leader region of the waxy gene was analyzed in a sample of 74 accessions, including Oryza sativa L. ssp. indica, japonica and wild rice (O.rufipogon) representing a wide distribution range of amylose content (AC) in cultivated rice. Eight alleles were detected in the (CT) n motif and two alleles were resolved in the (AATT) n motif. The distribution of the alleles of the two SSRs was quite uneven as detected by the (CT) n motif. The repeat numbers of the two SSR motifs, (CT) n and (AATT) n, appeared to be inversely related such that the total length of this region was maintained. AC of the varieties was highly correlated with the length of SSRs. Differences in AC among the various SSR genotypes were statistically highly significant as analyzed using genotypes of both SSR motifs. Although the SSR variation did not seem to have obvious function in the synthesis of the starch synthase encoded by the waxy gene, the almost perfect correlation between the two SSRs and AC level could be used for quality improvement in rice breeding programs.
基金Project supported by the Hi-Tech Research and Development Pro-gram (863) of China (No. 2004AA241180), and the Scientific Re-search Foundation for the Returned Overseas Chinese Scholars of State Education Ministry, and the Science and Technology Depart-ment of Zhejiang Province, China
文摘Sweetpotato starch thermal properties and its noodle quality were analyzed using a rapid predictive method based on near-infrared spectroscopy (NIRS). This method was established based on a total of 93 sweetpotato genotypes with diverse genetic background. Starch samples were scanned by NIRS and analyzed for quality properties by reference methods. Results of statistical modelling indicated that NIRS was reasonably accurate in predicting gelatinization onset temperature (To) (standard error of prediction SEP=2.014 ℃, coefficient of determination RSQ=0.85), gelatinization peak temperature (Tp) (SEP=-1.371 ℃, RSQ=0.89), gelatinization temperature range (Tr) (SEP=2.234 ℃, RSQ=0.86), and cooling resistance (CR) (SEP=0.528, RSQ=0.89). Gelatinization completion temperature (To), enthalpy of gelatinization (△H), cooling loss (CL) and swelling degree (SWD), were modelled less well with RSQ between 0.63 and 0.84. The present results suggested that the NIRS based method was sufficiently accurate and practical for routine analysis of sweetpotato starch and its noodle quality.
文摘This work is focused on the characterization and rapid analytical determination of cereal flour quality with regard to nutritional and breadmaking quality. Starch, protein and non-starch polysaccharides are the main components of cereals. The content and quality of proteins and content of damaged starch is important because of the technological quality of flours. The high content of high molecular weight proteins is substantial for bread technology especially, while soluble protein fractions and non-starch polysaccharides are important for nutrition. The set of wheat, barley and rye flours and their blends were analyzed and their properties and their qualitative parameters were determined. Principal component analysis (PCA) was used on Fourier transform-infrared (FT-IR) spectra in the 1,200-800 cm1 wavenumber region and significant correlations of various nutritional and breadmaking parameters were observed. Results showed that the FT-IR spectroscopy and PCA can serve for rapid screening and classification of cereal flour quality.
文摘The effect of varied processing variables (yam slice thickness, drying temperatures and type of drying) were investigated to determine their effects on the proximate and some functional properties of yam flour. The yam flour was produced with different slice thickness of 1, 1.5, 2.0, 2.5 and 5 cm using both conventional sun drying method and oven drying at temperatures; 40 ~C, 50 ~C, 60 ~C and 70 ~C. The moisture content of all the yam flour samples increased with increasing yam slice thickness, with the samples sun dried having the lowest values (6.20%-6.87%) followed by those dried at 70 ~C and then 60 ~C. The protein content of the yam flour samples increased with increase in slice thickness and decreased with increase in drying temperatures while the fat and the crude fibre of all the yam flour samples decreased with increase in slice thickness. The water absorption capacity of the sun dried yam flour samples decreased with increase in slice thickness while the syneresis value, bulk density and the gel strength increased with increase in slice thickness for all drying temperatures and for sun dried yam flour samples but smaller slice thickness had higher swelling capacity.
文摘Aloe gel, derived from Aloe vera plant is well known for its nutraceutical potential, It is available commercially as a juice with poor sensory quality. The therapeutic benefits of Aloe gel could be extended to food products concomitantly improving its acceptability. Dahi/curd is an Asian fermented dairy product that enjoys tremendous mass appeal. The objective of the present study was to formulate dahi enriched with Aloe gel (AG) as a functional ingredient and to study its effect on the quality of dahi. Plain dahi was prepared by addition of 2.5% skimmed milk powder (SMP) and suitable starter to milk. For the preparation of AG enriched dahi, SMP was replaced by AG at different levels (0.1-0.25%). The products were evaluated for moisture, pH, titrable acidity (TA), whey syneresis (WS), total solid content (TS), water holding capacity (WHC), total yield (TY), whiteness index (WI), and viscosity. Microbial analysis and sensory evaluation were also carried out. Enrichment of dahi with AG was found to improve its quality characteristics by causing a significant reduction in WS and an increase in TA, TS, WHC, TY, WI and viscosity. AG dahi recorded good sensory acceptability. Storage study also indicated AG dahi to perform better in terms of the various parameters studied. The present investigation suggests the feasibility of adding Aloe gel powder as a functional ingredient to dahi to enhance product quality as well as its nutritional and therapeutic potency. It could be commercially exploited as a novel product.