A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- ge...A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- gen ions, produced by a van de Graaff accelerator, in an en- ergy range of 2.0—3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is colli- mated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation proto- cols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.展开更多
MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis.So far,many miRNAs dete...MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis.So far,many miRNAs detection methods require polymerase chain reaction(PCR)amplification following reverse transcription of miRNAs.These processes are complicated and time-consuming.In this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4:Yb,Er upconversion nanoparticles.In this method,the fluorescence of NaYF_4:Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA detection.On the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.展开更多
文摘A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- gen ions, produced by a van de Graaff accelerator, in an en- ergy range of 2.0—3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is colli- mated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation proto- cols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.
基金the National Natural Science Foundation of China(61301039,21205036,31270908,61271056, 31540018)the Hunan Provincial Natural Science Foundation of China (13JJ4091)+2 种基金the Scientific Research Fund of Hunan Provincial Education Department(13A003)China Postdoctoral Science Foundation funded project(2014T70459)the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province[(2013) 448]
文摘MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis.So far,many miRNAs detection methods require polymerase chain reaction(PCR)amplification following reverse transcription of miRNAs.These processes are complicated and time-consuming.In this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4:Yb,Er upconversion nanoparticles.In this method,the fluorescence of NaYF_4:Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA detection.On the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.
