In order to modify inorganic particles as chromatic electrophoretic particles, an approach was designed and used to prepare Fe203 red electrophoretic particles. These Fe203-cationic hybrid nanoparticles （Fe203-CHNPs...In order to modify inorganic particles as chromatic electrophoretic particles, an approach was designed and used to prepare Fe203 red electrophoretic particles. These Fe203-cationic hybrid nanoparticles （Fe203-CHNPs）were prepared through Fe203 core covered with polymer shell which was composed of SiO2 and P （DMAEMA-co-HMA） by using atom transfer radical polymerization （ATRP）technique. The SiO：-coating could introduce the functional group on the surfaceof inorganic particles, through which the polymer shell could be formed by using ATRP tech- nique. The results of Fourier transform infrared spectra （FT-IR）, X-ray photoelectron spectroscopy （XPS） and thermal gravimetric analysis （TGA）confirmed the chemical compositions of Fe2O3-CHNPs; the images of transmission elec- tron microscopy （TEM） indicated the core-shell structure of Fe2O3-CHNPs; the measurements of dynamic light scatter- ing （DLS） showed a 253.7 nm average particle size with narrow size distribution; and the zeta potential measurements identified the high chargeability of Fe2O3-CHNPs. Furthermore, the resulting nanoparticles were successfully applied in the electrophoretic display cell, which demonstrated that it was an effective approach to preparing chromatic elec- trophoretic particles.展开更多
Objective： To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods： Two crucial conditions （sodium chloride concentration and amount of the magnetic...Objective： To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods： Two crucial conditions （sodium chloride concentration and amount of the magnetic particles） were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction （PCR） were performed. Results： The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion： The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.展开更多
文摘In order to modify inorganic particles as chromatic electrophoretic particles, an approach was designed and used to prepare Fe203 red electrophoretic particles. These Fe203-cationic hybrid nanoparticles （Fe203-CHNPs）were prepared through Fe203 core covered with polymer shell which was composed of SiO2 and P （DMAEMA-co-HMA） by using atom transfer radical polymerization （ATRP）technique. The SiO：-coating could introduce the functional group on the surfaceof inorganic particles, through which the polymer shell could be formed by using ATRP tech- nique. The results of Fourier transform infrared spectra （FT-IR）, X-ray photoelectron spectroscopy （XPS） and thermal gravimetric analysis （TGA）confirmed the chemical compositions of Fe2O3-CHNPs; the images of transmission elec- tron microscopy （TEM） indicated the core-shell structure of Fe2O3-CHNPs; the measurements of dynamic light scatter- ing （DLS） showed a 253.7 nm average particle size with narrow size distribution; and the zeta potential measurements identified the high chargeability of Fe2O3-CHNPs. Furthermore, the resulting nanoparticles were successfully applied in the electrophoretic display cell, which demonstrated that it was an effective approach to preparing chromatic elec- trophoretic particles.
基金Supported by the National High Technology Research and Development Program of China (2006AA020705)
文摘Objective： To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods： Two crucial conditions （sodium chloride concentration and amount of the magnetic particles） were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction （PCR） were performed. Results： The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion： The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.
