Neutrophils are the first cell type to arrive at the in-jury sites and play a critical role in host defense, by virtueof its ability to adhere and transmigrate through endothe-lium, to phagocytose foreign pathogens, a...Neutrophils are the first cell type to arrive at the in-jury sites and play a critical role in host defense, by virtueof its ability to adhere and transmigrate through endothe-lium, to phagocytose foreign pathogens, and to producefree oxygen radicals and proteolytic enzymes. Yet, inap-propriate neutrophil activation causes tissue damage andvarious inflammatory diseases. These physiological andpathological functions of neutrophils depend on the en-gagement of certain surface receptors, especially αMβ2,the ma jor β2 integrin receptor present on neutrophil sur-face. Understanding of the molecular mechanisms under-lying ligand binding by αMβ2, as well as the roles of αMβ2-ligand interactions in neutrophil functions will enable usto regulate more precisely neutrophil activities: that is,to promote their host defense functions, and at the sametime to minimize their deleterious effects on normal cells.展开更多
Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vi...Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.展开更多
Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied ...Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization. Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of prolifera-tive mesangium. Increased expression of α2 and β1 proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of α1, α2, β1 and γ1 increased to different degrees in glomeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:① The quantitative and qualitative alterations of laminin chains' distribution were found in the measngial proliferative glomeruli. The proliferative mesangial cells were the origins of abnormal accumulation and expression of laminins. ② These changes may be the basis of the progresses of MsPGN.展开更多
Objective: To observe the effect of Busui Shengxue Granule (补髓生血颗粒Herbal granule for replenishing marrow to produce blood) on chronic aplastic anemia (CAA) patients’ integrin α 6 (VLA-6/CD49f) and laminin (Ln)...Objective: To observe the effect of Busui Shengxue Granule (补髓生血颗粒Herbal granule for replenishing marrow to produce blood) on chronic aplastic anemia (CAA) patients’ integrin α 6 (VLA-6/CD49f) and laminin (Ln). Methods: Sixty-five patients were divided into experimental group and control group through random number table. There were 34 patients, 17 were male and 17 female, aged 2–67, with a medianage of 30.2 ± 8.6, in the experimental group, including 17 patients of kidney-yin deficiency and 17 of kidney-yang deficiency, treated by Busui Shengxue Granule. There were 31 patients in the control group,16 were male and 15 female, aged 4–65, with a medianage of 31.2 ± 8.0; administered Zaizhang Shengxue Tablet (再障生血片Herbal tablet for chronic aplastic anemia). Both groups were treated for six months and compared with 10 normal persons after the treatment. Flow cytometry was adopted to detect the change in the expression of VLA-6/CD49f, receptor in mononuclear cells of CAA patients and normal persons. Enzyme-linked immunosorbent assay was applied to detect the expression of peripheral serum Ln. Results: CAA patients’ VLA-6/CD49f was in the state of low expression and Ln in the state of high expression. After the treatment, both VLA-6/CD49f and Ln were regulated to some extent and the change in the experimental group was better than that of the control group. Compared with the kidney-yin deficiency patients, those indices of kidney-yang deficiency patients were easier to correct. Conclusion: The VLA-6/CD49f and Ln expressions of CAA patients are abnormal. The treatment with Busui Shengxue Granule makes both of them improved.展开更多
Mammalian cells express four different plasma membrane Ca2+ ATPases.Two of them(PMCA1 and PMCA4) are expressed ubiquitously,and are considered housekeeping isoforms.Two(PMCA2 and PMCA4) have tissue restricted distribu...Mammalian cells express four different plasma membrane Ca2+ ATPases.Two of them(PMCA1 and PMCA4) are expressed ubiquitously,and are considered housekeeping isoforms.Two(PMCA2 and PMCA4) have tissue restricted distribution.They are abundantly expressed in the brain and in nervous tissue-derived cell types.The primary transcripts of all PMCAs undergo alternative splicing,generating a large number of additional isoforms.Splicing occurs at site A,in the N-terminal moiety of the pump,and at site C,within the C-terminal calmodulin binding domain:The pumps are canonical targets of calmodulin stimu-lation.The site C insertion leads to a truncation of the pump about 50 residues short of the original C-terminal.One of the pumps(PMCA2) has special properties:It displays high activity even in the absence of the natural activator calmodulin,and has a particularly complex pattern of alternative splicing at both sites A and C.A variant of the PMCA2 pump containing an insert at site A and truncated C-terminally is the resident isoform of the pump in the stereocilia of hair cells of the inner ear.It exports Ca2+to the endolymph that bathes the stereocilia less efficiently than the full length,non-inserted PMCA2 pump.The proper functioning of hair cells demands the precise maintenance of the Ca2+balance between hair cells and the endolymph. Disturbances in the balance affect the process of mechano-electrical transduction,which depends on the ability of the stereo-ciliar bundle to deflect in response to sound waves.The tip links that organize the bundle are formed by the Ca2+binding pro-tein cadherin 23 and by protocadherin 15:Disturbances of the Ca2+binding by cadherin 23 and/or of the ability of the PMCA2 variant of the stereocilia to export Ca2+to the endolymph generate hearing loss phenotypes.Such phenotypes have now been described in mice and humans.In some cases they are linked to mutations of both cadherin 23 and the PMCA2 pump,but in other cases they may be generated by mutations of particular severity in only one of the two proteins.The PMCA2 defect that leads to deafness has now been analyzed molecularly:It affects the long range,unstimulated ability of PMCA2 to export Ca2+.展开更多
文摘Neutrophils are the first cell type to arrive at the in-jury sites and play a critical role in host defense, by virtueof its ability to adhere and transmigrate through endothe-lium, to phagocytose foreign pathogens, and to producefree oxygen radicals and proteolytic enzymes. Yet, inap-propriate neutrophil activation causes tissue damage andvarious inflammatory diseases. These physiological andpathological functions of neutrophils depend on the en-gagement of certain surface receptors, especially αMβ2,the ma jor β2 integrin receptor present on neutrophil sur-face. Understanding of the molecular mechanisms under-lying ligand binding by αMβ2, as well as the roles of αMβ2-ligand interactions in neutrophil functions will enable usto regulate more precisely neutrophil activities: that is,to promote their host defense functions, and at the sametime to minimize their deleterious effects on normal cells.
基金Supported by the National Natural Science Foundation of China(30800479)
文摘Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.
基金Supported by Grant from the Natural Science Foundation of Education Committee of Jiangsu Province(96047)
文摘Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization. Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of prolifera-tive mesangium. Increased expression of α2 and β1 proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of α1, α2, β1 and γ1 increased to different degrees in glomeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:① The quantitative and qualitative alterations of laminin chains' distribution were found in the measngial proliferative glomeruli. The proliferative mesangial cells were the origins of abnormal accumulation and expression of laminins. ② These changes may be the basis of the progresses of MsPGN.
基金supported by the the National Natural Science Foundation of China (No.90709039)Specialized Research Fund for the Doctoral Program of Higher Education (No.200802280003, No.20092327120001)Heilongjiang Provincial Natural Science Foundation of China (No.D2005-62)
文摘Objective: To observe the effect of Busui Shengxue Granule (补髓生血颗粒Herbal granule for replenishing marrow to produce blood) on chronic aplastic anemia (CAA) patients’ integrin α 6 (VLA-6/CD49f) and laminin (Ln). Methods: Sixty-five patients were divided into experimental group and control group through random number table. There were 34 patients, 17 were male and 17 female, aged 2–67, with a medianage of 30.2 ± 8.6, in the experimental group, including 17 patients of kidney-yin deficiency and 17 of kidney-yang deficiency, treated by Busui Shengxue Granule. There were 31 patients in the control group,16 were male and 15 female, aged 4–65, with a medianage of 31.2 ± 8.0; administered Zaizhang Shengxue Tablet (再障生血片Herbal tablet for chronic aplastic anemia). Both groups were treated for six months and compared with 10 normal persons after the treatment. Flow cytometry was adopted to detect the change in the expression of VLA-6/CD49f, receptor in mononuclear cells of CAA patients and normal persons. Enzyme-linked immunosorbent assay was applied to detect the expression of peripheral serum Ln. Results: CAA patients’ VLA-6/CD49f was in the state of low expression and Ln in the state of high expression. After the treatment, both VLA-6/CD49f and Ln were regulated to some extent and the change in the experimental group was better than that of the control group. Compared with the kidney-yin deficiency patients, those indices of kidney-yang deficiency patients were easier to correct. Conclusion: The VLA-6/CD49f and Ln expressions of CAA patients are abnormal. The treatment with Busui Shengxue Granule makes both of them improved.
文摘Mammalian cells express four different plasma membrane Ca2+ ATPases.Two of them(PMCA1 and PMCA4) are expressed ubiquitously,and are considered housekeeping isoforms.Two(PMCA2 and PMCA4) have tissue restricted distribution.They are abundantly expressed in the brain and in nervous tissue-derived cell types.The primary transcripts of all PMCAs undergo alternative splicing,generating a large number of additional isoforms.Splicing occurs at site A,in the N-terminal moiety of the pump,and at site C,within the C-terminal calmodulin binding domain:The pumps are canonical targets of calmodulin stimu-lation.The site C insertion leads to a truncation of the pump about 50 residues short of the original C-terminal.One of the pumps(PMCA2) has special properties:It displays high activity even in the absence of the natural activator calmodulin,and has a particularly complex pattern of alternative splicing at both sites A and C.A variant of the PMCA2 pump containing an insert at site A and truncated C-terminally is the resident isoform of the pump in the stereocilia of hair cells of the inner ear.It exports Ca2+to the endolymph that bathes the stereocilia less efficiently than the full length,non-inserted PMCA2 pump.The proper functioning of hair cells demands the precise maintenance of the Ca2+balance between hair cells and the endolymph. Disturbances in the balance affect the process of mechano-electrical transduction,which depends on the ability of the stereo-ciliar bundle to deflect in response to sound waves.The tip links that organize the bundle are formed by the Ca2+binding pro-tein cadherin 23 and by protocadherin 15:Disturbances of the Ca2+binding by cadherin 23 and/or of the ability of the PMCA2 variant of the stereocilia to export Ca2+to the endolymph generate hearing loss phenotypes.Such phenotypes have now been described in mice and humans.In some cases they are linked to mutations of both cadherin 23 and the PMCA2 pump,but in other cases they may be generated by mutations of particular severity in only one of the two proteins.The PMCA2 defect that leads to deafness has now been analyzed molecularly:It affects the long range,unstimulated ability of PMCA2 to export Ca2+.
