AIM: To investigate the expression and significance of caudal-related homeobox transcription factor (Cdx2) in gastric carcinoma (GC) and precancerous lesions. METHODS: The expression of Cdx2 in GC, precancer- ou...AIM: To investigate the expression and significance of caudal-related homeobox transcription factor (Cdx2) in gastric carcinoma (GC) and precancerous lesions. METHODS: The expression of Cdx2 in GC, precancer- ous lesions and normal gastric mucosa were detected using immunohistochemical method. Hematoxylin and eosin staining, alcian blue/periodic acid-schiff and high iron diamine/alcian blue staining were used to classify intestinal metaplasia (IM) and GC. RESULTS: Cdx2 was not detected in normal gas- tric mucosa. Cdx2 expression was detected in 87.1% (101/116) of IM, 50% (36/72) of dysplasia and 48.2% (41/85) of GC. The Cdx2-expressing cells in IM were more prevalent than in dysplasia and carcinoma (P 〈 0.05). There was no relationship between Cdx2 ex- pression and the classification of IM or the degree of dysplasia. Expression of Cdx2 was significantly higher in intestinal-type carcinoma than in diffuse and mixed- type carcinoma (P 〈 0.05). Positive expression of Cdx2was mainly found in moderately to well differentiated GC. There was a negative association between nuclear Cdx2 expression and lymph node metastasis and tumor, nodes, metastasis stage of GC (P 〈 0.05). The patients with Cdx2-positive expression showed a higher survival rate than those with Cdx2-negative expression (P = 0.038). Multivariate analysis revealed that the expres- sion of Cdx2 and lymph node metastasis were indepen- dent prognostic indicators of GC (P 〈 0.05).展开更多
Field experiments to evaluate four different colored sticky cards for trapping non-target insects were conducted in an organic maize field in the Heinigou region of China. Yellow, blue, green, and red sticky cards wer...Field experiments to evaluate four different colored sticky cards for trapping non-target insects were conducted in an organic maize field in the Heinigou region of China. Yellow, blue, green, and red sticky cards were used to trap insects in the field. The total number of insects species caught was 54, with 3,862 individuals recorded. Over half of the specimens caught were non-target insects, including phytophagous insects, particularly dipteran species(including many mosquitoes)(50.3%), followed by target pests(37.0%), and beneficial insects(12.7%). Statistical analysis revealed a significant difference in attraction to target pests, non-target pests, and beneficial insects among treatment groups. The results showed that higher numbers of target pests(Myzus persicae Sulzer, Empoasca flavescens Fabricius, Nysius ericaecshinly Schilling) were caught on yellow sticky card traps compared with blue, green, or red sticky card traps, indicating that yellow was the best trap color for target pests, with green and blue being progressively less attractive. For non-target insects, including phytophagous insects, flies, and mosquitoes,higher numbers of were caught on blue sticky card traps compared with yellow,green, or red sticky card traps. Our study indicated that blue was the most attractive color for flies, especially for the housefly, Musca domestica Linnaeus. Our study also showed that most beneficial insects exhibited preferences to particular trap color characteristics: yellow was the most attractive color for parasitic wasps and lady beetles; blue was the most attractive color for hoverflies and honeybees. In contrast,green and red had no significant attraction to beneficial insects.展开更多
AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling...AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon. RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di-and triacylglycerols. Among the meo tabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03). CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.展开更多
Soil shear strength is an important indicator of engineering design and an essential parameter of soil precision tillage and agricultural machinery and equipment design. Although numerous studies have investigated the...Soil shear strength is an important indicator of engineering design and an essential parameter of soil precision tillage and agricultural machinery and equipment design. Although numerous studies have investigated the characteristics of different soil shear strengths, only a few of these works have paid attention to soils containing considerable quantities of rock fragments. To date, most studies on the effects of rock fragments on the shear strength have paid attention to the role of rock fragments with sizes 〉2 mm. The effects of rock fragments 〈2 mm in soil are generally ignored. Similar to rock fragments 〉2 ram, the presence of rock fragments 〈2 mm could also change the mechanical properties of soils. Thus, in the present study we evaluated the potential influence of 〈2 mm rock fragments on soil shear strength via an unconsolidated undrained (UU) triaxial compression test. Our results were as follows: (1) A certain quantity of 〈2 mm rock fragments presented in purple soils developed from clay rocks; and an appropriate quantity of 〈2 mm rock fragments could improve the shear strength of soils. (2) The different PSDs of soils containin 〈2 mm rock fragment mainly caused variations in the internal friction angle of soils. (3) The shear strengths of the two mudstone-developed red-brown and gray-brown purple soils was more sensitive to water than that of the shale-developed coarse-dark purple soil. As the soil water content increased from 9% to 23%, the changes in the cohesion, internal friction angle, shear strength, and the maximum principal stress difference were smaller in the coarse dark purple soil than in the two other soils. We therefore concluded that 〈2 mm rock fragments in purple soils exerted important effects on soil shear strength. A better understanding of the differences among the shear strength features of purple soils could help improve the design of agricultural machinery and equipment.展开更多
The mobile factors of cracked ore in vibrating-ore draw shafts were analyzed. The results show that the mobile coefficient of cracked ores will be mainly influenced by the combination of ore physical factors if the st...The mobile factors of cracked ore in vibrating-ore draw shafts were analyzed. The results show that the mobile coefficient of cracked ores will be mainly influenced by the combination of ore physical factors if the structure dimension and parameters of vibrating ore-draw shafts are sure. It decreases with increasing the cohesion, lump content, lump size and powder content and increases with increasing the porosity. The coefficient decreases with increasing the moisture content, but increases after the moisture content reaches a certain value. Uniform grain leads to better mobility, non-uniform grain leads to worse mobility. The value of the mobile coefficient should be in a range of 0.3-1.1 when designing the vibrating ore-draw shafts. According to correlation degree of grey system (theory,) the effects of factors on the mobility of cracked ore are given in the weight decreasing consequence as follows: moisture content, lump content, distribution of grain size, lump size, porosity, cohesion and powder ore content. It is unreasonable to neglect any one because the values of their weights are not obvious.展开更多
The electrodeposition of black Ni-Co alloy film from Watts nickel solution and the effects of benzotriaozole and imidazole as the additives were studied. The electrolyte consists of NiSO4, NiC12, H3BO3, COSO4 and KNO3...The electrodeposition of black Ni-Co alloy film from Watts nickel solution and the effects of benzotriaozole and imidazole as the additives were studied. The electrolyte consists of NiSO4, NiC12, H3BO3, COSO4 and KNO3. The cathode current efficiency and the throwing power of the solution and the film adhesion to the mild steel metallic foil were determined by standard methods. The crystal structure, lattice parameter, crystal orientation and crystal size were analyzed by X-ray diffraction (XRD). Moreover, the surface morphology and elemental composition of the black Ni-Co alloy films were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) techniques. The darkness of the black films increases with increasing the incorporation of Co ion into the films. The XRD studies reveal that the black Ni-Co alloy films exhibit Ni (11 l) as the preferred orientation.展开更多
Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on t...Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on the basis of characteristics of penetration resistance and penetration depth curves, and the deterioration depth of cement soil with the cement ratio of 7%, reached 31.8 mm after 720 d. Results of research indicated that deterioration extended quickly under seawater environment and the deterioration depth increased with the prolonging curing time. In addition, the water pressure could speed up deterioration. With the increase of cement content, the strength of cement soil increased obviously. At the same time, the deterioration depth decreased significantly. The concentration of calcium ion in the cement stabilized soil increased with the increase of depth, while that of magnesium ion gradually decreased. The variations were consistent with energy dispersive spectrometer(EDS)analysis results, and the calcium concentration with depth was in a good consistency with strength distribution at long term. The results showed that the deterioration became more serious with the curing time, and it was related to calcium leaching.展开更多
A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS...A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS) and 10 ng ml4 basic fibroblast growth factor (bFGF). Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number (2n=46). The cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus (LCDV) and found to be susceptible to the virus in cytopathic effect (CPE) observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells.展开更多
This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five trea...This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to  ̄3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol (DTT)-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein (35 KDa) of the BEC.展开更多
Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA ...Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results: The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa (P 〈 0.05). Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group (P 〈 0.05). The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group (P 〈 0.001). Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion: The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it.展开更多
To investigate variation characteristics of interlayer water bound up with organic matter in smectite, organo-clay complexes extracted from grinded source rock samples were determined using thermo-XRD, DTA and PY-GC. ...To investigate variation characteristics of interlayer water bound up with organic matter in smectite, organo-clay complexes extracted from grinded source rock samples were determined using thermo-XRD, DTA and PY-GC. The dool diffraction peak of organo-clay complexes is postponed from 250 to 550℃ before reaching 1.00 nm and accompanied by exothermal peaks on DTA and organic matter with abundant C20-C30carbon detected by PY-GC, which is different from single smectite and indicates the existence of organic matter in the interlayer of smectite. Water desorption characteristics of organo-clay complexes are in consistent with smectite at 100 and 600℃, but different from smectite at 550℃ with an additional dehydration peak and a remaining d001 diffraction peak, suggesting the vcater removed at 550℃ is interlayer adsorption water rather than constituent water of clay minerals. Comparing the dehydration order and water loss, we conclude that part of interlayer water of smectite may act as the "bridge" that binds organic matter and smectite, which results in water-expelled lag beyond 250℃ and may provide a good medium for hydrocarbon migration and oil pool formation.展开更多
OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of athe...OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.展开更多
Crude oil hydrocarbon composition characteristics and oil viscosity prediction are important bases in petroleum exploration. A total of 54 oil/heavy-oil samples and 17 oil sands were analyzed and quantified using both...Crude oil hydrocarbon composition characteristics and oil viscosity prediction are important bases in petroleum exploration. A total of 54 oil/heavy-oil samples and 17 oil sands were analyzed and quantified using both comprehensive 2D gas chromatog raphy (GCxGC) and comprehensive 2D gas chromatography/time-of-flight mass spectrometry (GCxGC/TOFMS). The results show that crude oil in the West slope is mainly heavy oil and its hydrocarbon composition is characterized overall by paraf fins 〉 mono-aromatics 〉 naphthenes 〉 non-hydrocarbons 〉 di-aromatics 〉 tri-aromatics 〉 tetra-aromatics. Aromatics are most abundant and non-hydrocarbons are least abundant, whilst content differences among paraffins, naphthenes, aromatics, and non-hydrocarbons are less than 15%. There are two types of heavy oil, secondary type and mixing type. Biodegradation is the main formation mechanism of heavy oil. Biodegradation levels cover light biodegradation, moderate biodegradation, and se- vere biodegradation. With increasing biodegradation, paraffin content decreases while contents of aromatics and non- hydrocarbons increase. In contrast, naphthene content increases first and then decreases with increasing biodegradation. In se- vere biodegradation stage, naphthenes decrease more quickly than aromatics and non-hydrocarbons. This provides a new method for studying oil/heavy-oil biodegradation mechanism and biodegradation resistance of different hydrocarbons at dif- ferent biodegradation stages. In the Longhupao-Daan terrace and Qijia-Gulong depression, most crude oil is conventional oil. Its composition is dominated by paraffins with the lowest content of aromatics. In some casual oil wells from the Long- hupao-Daan terrace, crude oil from Saertu oil reservoirs is moderately biodegraded whereas crude oil from Putaohua oil reser voir is lightly biodegraded. Chemical parameters using saturate hydrocarbons and aromatics are usually not suitable for deter mining organic type and thermal maturity of biodegraded oil, especially of moderately or severely biodegraded oil, whilst Ts/(Ts+Tm) ratio can be used to determine thermal maturity of both conventional crude oil and heavy oil.展开更多
基金Supported by The Natural Science Foundation of Anhui Province,No. 090413118
文摘AIM: To investigate the expression and significance of caudal-related homeobox transcription factor (Cdx2) in gastric carcinoma (GC) and precancerous lesions. METHODS: The expression of Cdx2 in GC, precancer- ous lesions and normal gastric mucosa were detected using immunohistochemical method. Hematoxylin and eosin staining, alcian blue/periodic acid-schiff and high iron diamine/alcian blue staining were used to classify intestinal metaplasia (IM) and GC. RESULTS: Cdx2 was not detected in normal gas- tric mucosa. Cdx2 expression was detected in 87.1% (101/116) of IM, 50% (36/72) of dysplasia and 48.2% (41/85) of GC. The Cdx2-expressing cells in IM were more prevalent than in dysplasia and carcinoma (P 〈 0.05). There was no relationship between Cdx2 ex- pression and the classification of IM or the degree of dysplasia. Expression of Cdx2 was significantly higher in intestinal-type carcinoma than in diffuse and mixed- type carcinoma (P 〈 0.05). Positive expression of Cdx2was mainly found in moderately to well differentiated GC. There was a negative association between nuclear Cdx2 expression and lymph node metastasis and tumor, nodes, metastasis stage of GC (P 〈 0.05). The patients with Cdx2-positive expression showed a higher survival rate than those with Cdx2-negative expression (P = 0.038). Multivariate analysis revealed that the expres- sion of Cdx2 and lymph node metastasis were indepen- dent prognostic indicators of GC (P 〈 0.05).
基金Supported by the Misereor Foundation(grant ref:335-031-1028 Z)
文摘Field experiments to evaluate four different colored sticky cards for trapping non-target insects were conducted in an organic maize field in the Heinigou region of China. Yellow, blue, green, and red sticky cards were used to trap insects in the field. The total number of insects species caught was 54, with 3,862 individuals recorded. Over half of the specimens caught were non-target insects, including phytophagous insects, particularly dipteran species(including many mosquitoes)(50.3%), followed by target pests(37.0%), and beneficial insects(12.7%). Statistical analysis revealed a significant difference in attraction to target pests, non-target pests, and beneficial insects among treatment groups. The results showed that higher numbers of target pests(Myzus persicae Sulzer, Empoasca flavescens Fabricius, Nysius ericaecshinly Schilling) were caught on yellow sticky card traps compared with blue, green, or red sticky card traps, indicating that yellow was the best trap color for target pests, with green and blue being progressively less attractive. For non-target insects, including phytophagous insects, flies, and mosquitoes,higher numbers of were caught on blue sticky card traps compared with yellow,green, or red sticky card traps. Our study indicated that blue was the most attractive color for flies, especially for the housefly, Musca domestica Linnaeus. Our study also showed that most beneficial insects exhibited preferences to particular trap color characteristics: yellow was the most attractive color for parasitic wasps and lady beetles; blue was the most attractive color for hoverflies and honeybees. In contrast,green and red had no significant attraction to beneficial insects.
基金Supported by Valio Ltd and the Finnish Funding Agency for Technology and Innovation(TEKES)the preparation of this manuscript was funded in part by the Academy of Finland
文摘AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon. RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di-and triacylglycerols. Among the meo tabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03). CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.
基金supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (Grant No. 2012BAD05B06)
文摘Soil shear strength is an important indicator of engineering design and an essential parameter of soil precision tillage and agricultural machinery and equipment design. Although numerous studies have investigated the characteristics of different soil shear strengths, only a few of these works have paid attention to soils containing considerable quantities of rock fragments. To date, most studies on the effects of rock fragments on the shear strength have paid attention to the role of rock fragments with sizes 〉2 mm. The effects of rock fragments 〈2 mm in soil are generally ignored. Similar to rock fragments 〉2 ram, the presence of rock fragments 〈2 mm could also change the mechanical properties of soils. Thus, in the present study we evaluated the potential influence of 〈2 mm rock fragments on soil shear strength via an unconsolidated undrained (UU) triaxial compression test. Our results were as follows: (1) A certain quantity of 〈2 mm rock fragments presented in purple soils developed from clay rocks; and an appropriate quantity of 〈2 mm rock fragments could improve the shear strength of soils. (2) The different PSDs of soils containin 〈2 mm rock fragment mainly caused variations in the internal friction angle of soils. (3) The shear strengths of the two mudstone-developed red-brown and gray-brown purple soils was more sensitive to water than that of the shale-developed coarse-dark purple soil. As the soil water content increased from 9% to 23%, the changes in the cohesion, internal friction angle, shear strength, and the maximum principal stress difference were smaller in the coarse dark purple soil than in the two other soils. We therefore concluded that 〈2 mm rock fragments in purple soils exerted important effects on soil shear strength. A better understanding of the differences among the shear strength features of purple soils could help improve the design of agricultural machinery and equipment.
基金Project(50325415) supported by the National Science Fund for Distinguished Young Scholars project(50321402) sup ported by the National Science Fund for Innovative Research Group
文摘The mobile factors of cracked ore in vibrating-ore draw shafts were analyzed. The results show that the mobile coefficient of cracked ores will be mainly influenced by the combination of ore physical factors if the structure dimension and parameters of vibrating ore-draw shafts are sure. It decreases with increasing the cohesion, lump content, lump size and powder content and increases with increasing the porosity. The coefficient decreases with increasing the moisture content, but increases after the moisture content reaches a certain value. Uniform grain leads to better mobility, non-uniform grain leads to worse mobility. The value of the mobile coefficient should be in a range of 0.3-1.1 when designing the vibrating ore-draw shafts. According to correlation degree of grey system (theory,) the effects of factors on the mobility of cracked ore are given in the weight decreasing consequence as follows: moisture content, lump content, distribution of grain size, lump size, porosity, cohesion and powder ore content. It is unreasonable to neglect any one because the values of their weights are not obvious.
文摘The electrodeposition of black Ni-Co alloy film from Watts nickel solution and the effects of benzotriaozole and imidazole as the additives were studied. The electrolyte consists of NiSO4, NiC12, H3BO3, COSO4 and KNO3. The cathode current efficiency and the throwing power of the solution and the film adhesion to the mild steel metallic foil were determined by standard methods. The crystal structure, lattice parameter, crystal orientation and crystal size were analyzed by X-ray diffraction (XRD). Moreover, the surface morphology and elemental composition of the black Ni-Co alloy films were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) techniques. The darkness of the black films increases with increasing the incorporation of Co ion into the films. The XRD studies reveal that the black Ni-Co alloy films exhibit Ni (11 l) as the preferred orientation.
基金Supported by the National Natural Science Foundation of China(No.50779062)
文摘Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on the basis of characteristics of penetration resistance and penetration depth curves, and the deterioration depth of cement soil with the cement ratio of 7%, reached 31.8 mm after 720 d. Results of research indicated that deterioration extended quickly under seawater environment and the deterioration depth increased with the prolonging curing time. In addition, the water pressure could speed up deterioration. With the increase of cement content, the strength of cement soil increased obviously. At the same time, the deterioration depth decreased significantly. The concentration of calcium ion in the cement stabilized soil increased with the increase of depth, while that of magnesium ion gradually decreased. The variations were consistent with energy dispersive spectrometer(EDS)analysis results, and the calcium concentration with depth was in a good consistency with strength distribution at long term. The results showed that the deterioration became more serious with the curing time, and it was related to calcium leaching.
基金supported by grants from State 863High-Technology Rand Project of China(2006AA09Z406,2006AA10A401)Taishan Scholar Project of Shan-dong Province
文摘A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS) and 10 ng ml4 basic fibroblast growth factor (bFGF). Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number (2n=46). The cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus (LCDV) and found to be susceptible to the virus in cytopathic effect (CPE) observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells.
文摘This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to  ̄3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol (DTT)-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein (35 KDa) of the BEC.
基金Supported by a grant from Sanitary Science and Technological Development Foundation of Shandong Province (No. 2003HW015)
文摘Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results: The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa (P 〈 0.05). Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group (P 〈 0.05). The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group (P 〈 0.001). Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion: The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it.
基金supported by National Natural Science Foundation of China(Grant Nos. 40672085 and 40872089)National Oil and Gas Special Fund(Grant No. 2008ZX05023-003)+1 种基金Science and Technology Office Fund of China Petroleum & Chemical Co. Ltd. (Grant No. P08039)Program of the State Key Laboratory of Oil-Gas of China Petroleum University,Beijing (Grant No. P08026)
文摘To investigate variation characteristics of interlayer water bound up with organic matter in smectite, organo-clay complexes extracted from grinded source rock samples were determined using thermo-XRD, DTA and PY-GC. The dool diffraction peak of organo-clay complexes is postponed from 250 to 550℃ before reaching 1.00 nm and accompanied by exothermal peaks on DTA and organic matter with abundant C20-C30carbon detected by PY-GC, which is different from single smectite and indicates the existence of organic matter in the interlayer of smectite. Water desorption characteristics of organo-clay complexes are in consistent with smectite at 100 and 600℃, but different from smectite at 550℃ with an additional dehydration peak and a remaining d001 diffraction peak, suggesting the vcater removed at 550℃ is interlayer adsorption water rather than constituent water of clay minerals. Comparing the dehydration order and water loss, we conclude that part of interlayer water of smectite may act as the "bridge" that binds organic matter and smectite, which results in water-expelled lag beyond 250℃ and may provide a good medium for hydrocarbon migration and oil pool formation.
基金Supported by Research Grant from the Health Bureau of Shanghai(No.2008J003A)
文摘OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.
基金supported by National Basic Research Program of China(Grant No.2006CB701404)CNPC Grand S&T Special Project(Grant No.2012E-2603)
文摘Crude oil hydrocarbon composition characteristics and oil viscosity prediction are important bases in petroleum exploration. A total of 54 oil/heavy-oil samples and 17 oil sands were analyzed and quantified using both comprehensive 2D gas chromatog raphy (GCxGC) and comprehensive 2D gas chromatography/time-of-flight mass spectrometry (GCxGC/TOFMS). The results show that crude oil in the West slope is mainly heavy oil and its hydrocarbon composition is characterized overall by paraf fins 〉 mono-aromatics 〉 naphthenes 〉 non-hydrocarbons 〉 di-aromatics 〉 tri-aromatics 〉 tetra-aromatics. Aromatics are most abundant and non-hydrocarbons are least abundant, whilst content differences among paraffins, naphthenes, aromatics, and non-hydrocarbons are less than 15%. There are two types of heavy oil, secondary type and mixing type. Biodegradation is the main formation mechanism of heavy oil. Biodegradation levels cover light biodegradation, moderate biodegradation, and se- vere biodegradation. With increasing biodegradation, paraffin content decreases while contents of aromatics and non- hydrocarbons increase. In contrast, naphthene content increases first and then decreases with increasing biodegradation. In se- vere biodegradation stage, naphthenes decrease more quickly than aromatics and non-hydrocarbons. This provides a new method for studying oil/heavy-oil biodegradation mechanism and biodegradation resistance of different hydrocarbons at dif- ferent biodegradation stages. In the Longhupao-Daan terrace and Qijia-Gulong depression, most crude oil is conventional oil. Its composition is dominated by paraffins with the lowest content of aromatics. In some casual oil wells from the Long- hupao-Daan terrace, crude oil from Saertu oil reservoirs is moderately biodegraded whereas crude oil from Putaohua oil reser voir is lightly biodegraded. Chemical parameters using saturate hydrocarbons and aromatics are usually not suitable for deter mining organic type and thermal maturity of biodegraded oil, especially of moderately or severely biodegraded oil, whilst Ts/(Ts+Tm) ratio can be used to determine thermal maturity of both conventional crude oil and heavy oil.