期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Poly NP试验及MPNP试验快速检测肺炎克雷伯菌多粘菌素类耐药表型的应用评估 被引量:2
1
作者 刘衍伶 曾令兵 +3 位作者 胡妮娅 陈开森 胡雪飞 胡龙华 《中国临床药理学与治疗学》 CAS CSCD 2019年第6期650-657,共8页
目的:探讨快速多粘菌素NP试验(rapid polymyxin NP test,Poly NP test)对多粘菌素类耐药肺炎克雷伯菌的检测价值;进一步评估改良快速多粘菌素NP试验(modified rapid polymyxin Nordmann/Poirel test,MPNP)对产MCR肺炎克雷伯菌的检出价... 目的:探讨快速多粘菌素NP试验(rapid polymyxin NP test,Poly NP test)对多粘菌素类耐药肺炎克雷伯菌的检测价值;进一步评估改良快速多粘菌素NP试验(modified rapid polymyxin Nordmann/Poirel test,MPNP)对产MCR肺炎克雷伯菌的检出价值。方法:本研究共纳入48株菌,包括14株多粘菌素类敏感肺炎克雷伯菌(polymyxin sensitive Klebsiella pneumoniae ,PolS-KP),29株多粘菌素类耐药细菌(26株为多粘菌素类耐药肺炎克雷伯菌(polymyxin resistant Klebsiella pneumoniae ,PolR-KP),3株为多粘菌素类天然耐药菌),以及5株非发酵菌。以微量肉汤稀释法的结果为标准,采用Poly NP试验检测多粘菌素类的药敏表型。PCR及测序确定肺炎克雷伯菌的多粘菌素类染色体介导的耐药基因 pmrA/pmrB,phoP/phoQ,mgrB和质粒介导的耐药基因 mcr-1~mcr-8 ,采用MPNP试验检测是否为产MCR阳性菌株。结果:29株多粘菌素类耐药菌,3株天然耐药细菌的Poly NP试验均为阳性;26株PolR-KP中,24株为Poly NP试验阳性,2株为阴性;14株PolS-KP均为阴性,且所有结果均在2h内出现最终颜色变化。该方法判断肺炎克雷伯菌多粘菌素类耐药表型的敏感性和特异性为 93.1%和100%。26株PolR-KP中,有4株为MCR阳性,其中3株产 mcr-1,1株产mcr-8;22株为染色体介导的耐药,其中12株为双组分系统PmrAB突变,4株为双组分系统PhoPQ突变,6株为mgrB基因改变。4株MCR阳性菌株的MPNP试验表现为阴性结果,其余非MCR株的PolR-KP和PolS-KP,以及多粘菌素类天然耐药菌株的MPNP均表现为阴性结果。MPNP试验不能筛选出MCR阳性的肺炎克雷伯菌。结论:Poly NP试验快速,特异性强,敏感性高,可用于临床多粘菌素类耐药肺炎克雷伯菌的快速检测。MPNP试验对MCR阳性肺炎克雷伯菌检出不佳,仍需进一步探讨适用于肺炎克雷伯菌的MCR快速表型检测方法。 展开更多
关键词 粘菌 粘菌素耐药 肺炎克雷伯菌 快速检测 表型检测
下载PDF
Genomic insights into the ESBL and MCR-l-producing ST648 Escherichia coli with multi-drug resistance 被引量:5
2
作者 Huimin Zhang Christopher H. Seward +2 位作者 Zuowei Wu Huiyan Ye Youjun Feng 《Science Bulletin》 SCIE EI CAS CSCD 2016年第11期875-878,共4页
Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram- negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin ... Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram- negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene (mcr-1) is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-l-positive bacterial host reservoirs. Here we report genome sequencing of three human isolates of the mcr-l-positive Escherichia coli (E15004, E15015 and E15017) and define general features through analyses of bacterial comparative genomics. Fur- ther genomic mining together with sequence typing allowed us to elucidate that the MCR-l-carrying E. coli E15017 belongs to the sequence type ST648 and copro- duces extended-spectrum β-1actamase (ESBL). Given the fact that ST648 has been known to associate New Delhi metallo-β-1actamase 1 or ESBL, with either our results highlighted the possibility of ST648 as an epidemic clone with multidrug resistances. 展开更多
关键词 MCR-1 Extended-spectrum beta-lactam(ESBL) Colistin resistance ST648
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部