目的:通过系统评价的方式,证明在拔牙窝内放置氯己定(Chlorhexidine,CHX)生物粘附凝胶可以减少下颌第三磨牙拔除术后干槽症(Alveolar Osteitis,AO)的发生。方法:笔者使用计算机在Pubmed/Medline、Web Of Science搜索引擎以及Cochrane图...目的:通过系统评价的方式,证明在拔牙窝内放置氯己定(Chlorhexidine,CHX)生物粘附凝胶可以减少下颌第三磨牙拔除术后干槽症(Alveolar Osteitis,AO)的发生。方法:笔者使用计算机在Pubmed/Medline、Web Of Science搜索引擎以及Cochrane图书馆进行了详尽搜索,并对自2005年至今近10年来关于在拔牙窝内放置浓度氯己定生物粘附凝胶预防下颌第三磨牙拔除术后干槽症发生的随机对照试验(Randomized Controlled Trial,RCT)的文章进行收集。由两名研究人员使用Grade系统分别独立进行文献质量评价和数据提取。提取所得数据使用Revman5.3软件进行系统评价。结果:此次研究最终选定了6篇符合选择标准的文献(总共包括下颌第三磨牙拔除案例708例,其中实验组356例在拔牙术后于拔牙窝内放置了0.2%浓度氯己定生物粘附凝胶,对照组352例分别使用各自的安慰剂或空白对照)。根据异质性检验的结果(Chi2=9.78,df=5,P=0.08;I2=49%)笔者选用了固定效应分析模型进行分析。分析结果显示:于拔牙窝内放置0.2%氯己定生物粘附凝胶能够在下颌第三磨牙拔除术后有效预防干槽症的发生(RR=0.5,95%CI:0.37-0.69),并且该治疗方法能够在下颌第三磨牙拔除术后有效缓解拔牙创疼痛(术后第1天:MD=-0.81,95%CI=-1.14--0.17;术后第3天:MD=-1.06,95%CI=-1.39--0.73)。结论:拔牙窝内放置0.2%氯己定生物粘附凝胶能够有效预防下颌第三磨牙拔除术后干槽症的发生,且能够一定程度上缓解拔牙术后疼痛程度,减少患者术后的痛苦。展开更多
Oral mucositis due to chemotherapy and irradiation continues to be an important clinical problem. The effectiveness of hyaluronic acid-based compounds in accelerated healing and helping manage pain in patients with or...Oral mucositis due to chemotherapy and irradiation continues to be an important clinical problem. The effectiveness of hyaluronic acid-based compounds in accelerated healing and helping manage pain in patients with oral mucositis was demonstrated. It was investigated a protective and regenerative effect of hyaluronic acid based gel formulation enriched with NAG （N-acetyl glucosamine） loaded solid lipid nanoparticles against degenerative process of the oral mucosa. Gel formulations were obtained by adding sHA （sodium hyaluronate） into SLN aqueous suspension. Gel performances were evaluated by multi-methodological approach： mucoadhesive and barrier properties evaluation, cell viability. It was shown that gel formulation based sHA, enriched with NAG loaded SLNs, when added as suspension, demonstrated to have a good mucoadhesion profile comparable in terms of tensile work and fracture strength to Carbomer 934 2%. The presence of NAG encapsulated and not in gel formulation enhances also the biocompatibility of the system, demonstrating also to have a proliferative effect. Finally, any barrier property was altered. Finally, results demonstrated that sHA based gel formulation enriched SLNs, demonstrated good mucoadeshion property, comparable to carbopol gel, positive control. The proposed gel formulation enriched with SLNs setting up in this work could be used as innovation strategy to treat oral mucositis.展开更多
Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photol...Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly（ethylene glycol） hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was char- acterized by GPC and ~H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman＇s reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes.展开更多
文摘Oral mucositis due to chemotherapy and irradiation continues to be an important clinical problem. The effectiveness of hyaluronic acid-based compounds in accelerated healing and helping manage pain in patients with oral mucositis was demonstrated. It was investigated a protective and regenerative effect of hyaluronic acid based gel formulation enriched with NAG （N-acetyl glucosamine） loaded solid lipid nanoparticles against degenerative process of the oral mucosa. Gel formulations were obtained by adding sHA （sodium hyaluronate） into SLN aqueous suspension. Gel performances were evaluated by multi-methodological approach： mucoadhesive and barrier properties evaluation, cell viability. It was shown that gel formulation based sHA, enriched with NAG loaded SLNs, when added as suspension, demonstrated to have a good mucoadhesion profile comparable in terms of tensile work and fracture strength to Carbomer 934 2%. The presence of NAG encapsulated and not in gel formulation enhances also the biocompatibility of the system, demonstrating also to have a proliferative effect. Finally, any barrier property was altered. Finally, results demonstrated that sHA based gel formulation enriched SLNs, demonstrated good mucoadeshion property, comparable to carbopol gel, positive control. The proposed gel formulation enriched with SLNs setting up in this work could be used as innovation strategy to treat oral mucositis.
基金supported by the National Natural Science Foundation of China(21034002,91127028,51273046)the Ministry of Science and Technology of China(2011CB606203)the Science and Technology Development Foundation of Shanghai(13XD1401000)
文摘Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly（ethylene glycol） hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was char- acterized by GPC and ~H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman＇s reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes.
