Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vi...Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.展开更多
Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modific...Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modifications of proteins with phosphates, lipids, sugars or other residues confer on these proteins additional structural and functional diversity. For instance, protein phosphorylation is involved in a wide range of cellular processes including signal transduction. Protein glycosylation is one of the most abundant PTMs and more than 50% of all human proteins are glycosylated. Glycoproteins are involved in many biological events, such as cell-cell adhesion, communication, immune response and development.展开更多
基金Supported by the National Natural Science Foundation of China(30800479)
文摘Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.
文摘Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modifications of proteins with phosphates, lipids, sugars or other residues confer on these proteins additional structural and functional diversity. For instance, protein phosphorylation is involved in a wide range of cellular processes including signal transduction. Protein glycosylation is one of the most abundant PTMs and more than 50% of all human proteins are glycosylated. Glycoproteins are involved in many biological events, such as cell-cell adhesion, communication, immune response and development.
