The selection and compatibility of the microbial strains and bedding materials in a deep-litter system is the primary issues for this ecological breeding technology. In this paper, we analyzed and summarized the categ...The selection and compatibility of the microbial strains and bedding materials in a deep-litter system is the primary issues for this ecological breeding technology. In this paper, we analyzed and summarized the categories of microbial strains and bedding materials suitable for a deep-litter system, the fermentation properties of different microbes, the parameter requirements of bedding materials, and the fermentation process led by functional microbial flora in a deep-litter system, with the objective to provide theoretical bases and practical guidance for the promotion of deep-litter breeding method nationwide.展开更多
[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determ...[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.展开更多
Zea mays var. saccharata （sweet corn） is an important crops grown for food and feed in the tropics. In Indonesia, most of the land used for crops production is characterized by acid pH and low levels of nitrogen and...Zea mays var. saccharata （sweet corn） is an important crops grown for food and feed in the tropics. In Indonesia, most of the land used for crops production is characterized by acid pH and low levels of nitrogen and phosphorus. Phosphorus fertilizers have been widely used to improve soil fertility in Indonesia. Given the high cost of superphosphate （SP）, the focus is now on rock phosphate （RP） fertilizer, which is relatively cheaper than SP and shows high solubility in acid condition. The combination of RP and manure during the decomposing process and the addition of decomposing microbes may be a promising technique to overcome the problem. A field experiment was conducted on a vertisol soil （low pH and low available nitrogen and phosphorus）. The objective of the research is to evaluate the effect of combinations of manure enriched with RP （granular and non-granular） with three different decomposing microbes （EM4, starTmik and stardec） on sweet corn and dry matter production, phosphorus （P） and calcium （Ca） content in above-ground biomass. A completely randomized design with seven treatments and four replicates was used in this experiment. The size of the plots was 3 m x 2.5 m. The treatments were TO （manure）, T1 （manure ＋ EM4）, T2 （manure ＋ starTmik）, T3 （manure ＋ stardec）, T4 （manure granular ~ EM4）, T5 （manure granular ＋ starTmik）, T6 （manure granular ＋ stardec）. The manure was enriched with RP 66 kg P/ha （150 kg P2Os/ha） and inoculated with the biodecomposer according to the assigned treatment. The manure was applied in a rate of 30 t/ha. All plot received 125 kg KC1/ha （150 kg K20/ha） and 200 kg urea/ha as basal fertilization. Sweet corn was harvested 70 d after planting. Sweet corn plants were cut close to the ground and analyzed for dry matter yield （DM） and P and Ca content in the biomass. Result showed that sweet corn and DM yield and P content were not significantly influenced by the treatments. Ca content in the above-ground biomass, in turn, differed among the treatments. Manure enriched with RP fertilizer was not significantly diferent on Ca content of stover. Thus, it seems that no gain occurs in sweet corn production and nutritive value of stover from the addition of microbes to manure enriched with RP in a acid vertisol soil. In conclusion, manure enriched with RP with or without biodecomposer, in granular or non-granular form, resulted in similar on sweet corn and DM production, P and Ca content of stover.展开更多
基金Supported by the Special Fund for the Independent Innovation of Agricultural Sciences and Technology in Jiangsu Province[cx(12)1001-04]~~
文摘The selection and compatibility of the microbial strains and bedding materials in a deep-litter system is the primary issues for this ecological breeding technology. In this paper, we analyzed and summarized the categories of microbial strains and bedding materials suitable for a deep-litter system, the fermentation properties of different microbes, the parameter requirements of bedding materials, and the fermentation process led by functional microbial flora in a deep-litter system, with the objective to provide theoretical bases and practical guidance for the promotion of deep-litter breeding method nationwide.
基金Supported by Foundation of Henan Educational Committee(2010B530001)~~
文摘[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.
文摘Zea mays var. saccharata （sweet corn） is an important crops grown for food and feed in the tropics. In Indonesia, most of the land used for crops production is characterized by acid pH and low levels of nitrogen and phosphorus. Phosphorus fertilizers have been widely used to improve soil fertility in Indonesia. Given the high cost of superphosphate （SP）, the focus is now on rock phosphate （RP） fertilizer, which is relatively cheaper than SP and shows high solubility in acid condition. The combination of RP and manure during the decomposing process and the addition of decomposing microbes may be a promising technique to overcome the problem. A field experiment was conducted on a vertisol soil （low pH and low available nitrogen and phosphorus）. The objective of the research is to evaluate the effect of combinations of manure enriched with RP （granular and non-granular） with three different decomposing microbes （EM4, starTmik and stardec） on sweet corn and dry matter production, phosphorus （P） and calcium （Ca） content in above-ground biomass. A completely randomized design with seven treatments and four replicates was used in this experiment. The size of the plots was 3 m x 2.5 m. The treatments were TO （manure）, T1 （manure ＋ EM4）, T2 （manure ＋ starTmik）, T3 （manure ＋ stardec）, T4 （manure granular ~ EM4）, T5 （manure granular ＋ starTmik）, T6 （manure granular ＋ stardec）. The manure was enriched with RP 66 kg P/ha （150 kg P2Os/ha） and inoculated with the biodecomposer according to the assigned treatment. The manure was applied in a rate of 30 t/ha. All plot received 125 kg KC1/ha （150 kg K20/ha） and 200 kg urea/ha as basal fertilization. Sweet corn was harvested 70 d after planting. Sweet corn plants were cut close to the ground and analyzed for dry matter yield （DM） and P and Ca content in the biomass. Result showed that sweet corn and DM yield and P content were not significantly influenced by the treatments. Ca content in the above-ground biomass, in turn, differed among the treatments. Manure enriched with RP fertilizer was not significantly diferent on Ca content of stover. Thus, it seems that no gain occurs in sweet corn production and nutritive value of stover from the addition of microbes to manure enriched with RP in a acid vertisol soil. In conclusion, manure enriched with RP with or without biodecomposer, in granular or non-granular form, resulted in similar on sweet corn and DM production, P and Ca content of stover.
