[Objective] In order to provide firsthand information for the development of fluorescent histology,the paper studied the fluorescent features of ovarioles and germline cysts of fruit fly after common fixation and stai...[Objective] In order to provide firsthand information for the development of fluorescent histology,the paper studied the fluorescent features of ovarioles and germline cysts of fruit fly after common fixation and staining. [Method] With ovaries of Drosophila melanogaster as materials,after fixation with Bouin＇s fluid,the ovaries were sectioned and stained with HE,haematoxylin or eosin,respectively. The specimens were observed and photographed under fluorescent microscope in bright field,and fluorescent fields after excitation with green,blue and UV light,respectively. [Result] After staining by three methods,germ cells and somatic cells emitted different colors of fluorescence after excitation by different lights; lipids,nucleic acids and proteins in cells could also emit their special fluorescence. [Conclusion] Conventional dyes could give different fluorescence characteristics to germ cells and somatic cells,which can also give special fluorescence characteristics to different cellular components. Thus,the fluorescence histology will provide broad prospect for more convenient study on different cell types and cellular components.展开更多
Underground coal mining inevitably results in land surface subsidence.Acquiring information on land surface subsidence is important in the detection of surface change.However,conventional data acquisition techniques c...Underground coal mining inevitably results in land surface subsidence.Acquiring information on land surface subsidence is important in the detection of surface change.However,conventional data acquisition techniques cannot always retrieve information on whole subsidence area.This study focuses on the reconstruction of a digital elevation model（DEM） with terrestrial laser scanning（TLS） point cloud data.Firstly,the methodology of the DEM with terrestrial 3-dimensional laser scanning is introduced.Then,a DEM modeling approach that involves the application of curved non-uniform rational B-splines（NURBS） surface is put forward.Finally,the performance of the DEM modeling approach with different surface inverse methods is demonstrated.The results indicate that the DEM based on the point cloud data and curved NURBS surface can achieve satisfactory accuracy.In addition,the performance of the hyperbolic paraboloid appears to be better than that of the elliptic paraboloid.The reconstructed DEM is continuous and can easily be integrated into other programs.Such features are of great importance in monitoring dynamic ground surface subsidence.展开更多
The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,st...The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,stitching and overlay of this system are evaluated.The system can write complex patterns at dimensions down to 30nm.The demonstrated overlay accuracy of this system is better than 40nm.展开更多
Green fluorescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the living generative and sperm cells of a third generation transgenic rice (Oryza sativa L.) plant, ...Green fluorescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the living generative and sperm cells of a third generation transgenic rice (Oryza sativa L.) plant, A005-G-T-1-2. Observations were made on pollen at four major developmental stages, viz. I. uni-nucleate microspore stage; II. early bi-cellular pollen stage; III. late bi-cellular pollen stage; and IV. tri-cellular pollen stage. At each of these developmental stages vegetative nucleus, generative nucleus/ cell, and sperm cells were seen undergoing continuous and coordinated motion and migration. These movements seemed to be influenced by associated microfilament networks existing in the pollen. Based on these observations we propose that it is the interaction between the microfilament networks (usually one existing in the central cytoplasm and another in the cortex) that controls the dynamic movement of the vegetative nucleus, generative nucleus/cell and sperm cells. Furthermore, we have also observed that there is an array of microfilaments (oriented mostly parallel to the long axis of the cell) existing in the generative and sperm cells. As far as we are aware, this is the first report showing the existence of microfilaments in living generative and sperm cells of rice pollen. The implication and significance of the existence of microfilaments in generative and sperm cells in rendering self-propelled motion of these cells in relation to their passage and movement in the pollen tube and embryo sac for fertilization were discussed.展开更多
基金Supported by Natural Science Foundation of Shandong Province(Y2007D06)~~
文摘[Objective] In order to provide firsthand information for the development of fluorescent histology,the paper studied the fluorescent features of ovarioles and germline cysts of fruit fly after common fixation and staining. [Method] With ovaries of Drosophila melanogaster as materials,after fixation with Bouin＇s fluid,the ovaries were sectioned and stained with HE,haematoxylin or eosin,respectively. The specimens were observed and photographed under fluorescent microscope in bright field,and fluorescent fields after excitation with green,blue and UV light,respectively. [Result] After staining by three methods,germ cells and somatic cells emitted different colors of fluorescence after excitation by different lights; lipids,nucleic acids and proteins in cells could also emit their special fluorescence. [Conclusion] Conventional dyes could give different fluorescence characteristics to germ cells and somatic cells,which can also give special fluorescence characteristics to different cellular components. Thus,the fluorescence histology will provide broad prospect for more convenient study on different cell types and cellular components.
基金Project(51174206)supported by the National Natural Science Foundation of ChinaProject(2014ZDPY29)supported by the Fundamental Research Funds for the Central UniversitiesProject(SZBF 2011-6-B35)supported by the Priority Academic Program Development of Higher Education Institutions(PAPD)of Jiangsu Province,China
文摘Underground coal mining inevitably results in land surface subsidence.Acquiring information on land surface subsidence is important in the detection of surface change.However,conventional data acquisition techniques cannot always retrieve information on whole subsidence area.This study focuses on the reconstruction of a digital elevation model（DEM） with terrestrial laser scanning（TLS） point cloud data.Firstly,the methodology of the DEM with terrestrial 3-dimensional laser scanning is introduced.Then,a DEM modeling approach that involves the application of curved non-uniform rational B-splines（NURBS） surface is put forward.Finally,the performance of the DEM modeling approach with different surface inverse methods is demonstrated.The results indicate that the DEM based on the point cloud data and curved NURBS surface can achieve satisfactory accuracy.In addition,the performance of the hyperbolic paraboloid appears to be better than that of the elliptic paraboloid.The reconstructed DEM is continuous and can easily be integrated into other programs.Such features are of great importance in monitoring dynamic ground surface subsidence.
文摘The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,stitching and overlay of this system are evaluated.The system can write complex patterns at dimensions down to 30nm.The demonstrated overlay accuracy of this system is better than 40nm.
文摘Green fluorescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the living generative and sperm cells of a third generation transgenic rice (Oryza sativa L.) plant, A005-G-T-1-2. Observations were made on pollen at four major developmental stages, viz. I. uni-nucleate microspore stage; II. early bi-cellular pollen stage; III. late bi-cellular pollen stage; and IV. tri-cellular pollen stage. At each of these developmental stages vegetative nucleus, generative nucleus/ cell, and sperm cells were seen undergoing continuous and coordinated motion and migration. These movements seemed to be influenced by associated microfilament networks existing in the pollen. Based on these observations we propose that it is the interaction between the microfilament networks (usually one existing in the central cytoplasm and another in the cortex) that controls the dynamic movement of the vegetative nucleus, generative nucleus/cell and sperm cells. Furthermore, we have also observed that there is an array of microfilaments (oriented mostly parallel to the long axis of the cell) existing in the generative and sperm cells. As far as we are aware, this is the first report showing the existence of microfilaments in living generative and sperm cells of rice pollen. The implication and significance of the existence of microfilaments in generative and sperm cells in rendering self-propelled motion of these cells in relation to their passage and movement in the pollen tube and embryo sac for fertilization were discussed.
