长吻鮠精子保存液在人工授精中的应用试验

分别在安徽长江流域的无为和淮河流域的滁州进行长吻鮠的人工繁殖,使用LRH-A2、PG、混合激素B型、DOM 进行催产,从长江捕获的亲鱼立即催产,其催产率为66．67％,受精率80％-85％,经培育选择后催产率为83．33％,受精率65%- 87％。在滁州经多年培育的亲鱼催产率为100％,受精率78％-95％。精子保存液在精子保存和人工授精中使用效果良好。在冰桶内保存,20-30 h仍能保持良好的受精能力。

椰汁:理想的精子保存液

巴西农牧业科研所的科学家说,椰汁是世界上保存用于人工授精的精子的最好液体,这项发明目前已获专利。 尽管目前还只是用于保存动物的精子,但是,科研所的专家们说,在保存人的精子的应用项目上也有所突破。 据巴西科研人员说,这种保护液体。

基于SCSA乌苏里拟鲿精子保存液的使用效果

鱼类精子质量是影响人工繁殖的重要因素,雌雄性成熟不同步或需杀雄鱼取精的鱼类,为了保障其规模化繁殖,采用精子保存液将精子暂存起来,繁殖时随用随取,因此,精子保存液的保存效果又直接影响着人工繁殖。如何确定保存液中精子的质量,可以用镜检宏观判断,但这样并不精准。SCSA通过量化判断出保存液中精子的质量,从而为繁殖提供保障。自行研制的hnscz拟鲿精子保存液经SCSA分析测试,其实际应用优于购买的hanks精子保存液。测试结果为at≤0.15,精子活力旺盛,使用效果明显。

南方鲇精子保存方法的初步研究

以精子被激活后的活力为指标 ,对 6种精子保存液保存南方鲇的效果进行了评估 ,并对室温 (18～2 0℃ )和低温 (0～ 4℃ )状态下保存南方鲇精子的效果进行了比较研究。结果表明 ,在 6种精子保存液中 ,以添加牛血蛋白和氨基酸等营养物质的F精子保存液效果最好 ,精子的活力和寿命保持时间最长 ,6d内可保存 80 %以上活力 ,寿命持续 12 .5d。

Effect of Sources and Concentrations of Soybean Phosphatidylcholine on Diluted Goat Semen Equilibrated at 4 ℃

The objective of the present study was to determine the proper sources and concentrations of soybean lecithin （phosphatidylcholine, PC） to be used as substitute for hen egg yolk in extender for preserving goat semen. Two sources of soybean lecithin （20% and 95% soybean phosphatidylcholine; PC20 and PC95） and three concentrations （1%, 2% and 3% v/v） of PC20 and PC95 supplemented in Tris-citric acid-fructose （TCF） extender were tested. The TCF extender supplemented with 20% hen egg yolk was used as a control. Fresh semen samples were collected from 3 goats by artificial vagina. Seminal plasma was removed by centrifugation and sperm pellets were pooled together and divided into 7 groups according to types of extender. The diluted semen samples were kept at 4 ℃ （equilibration）. The semen qualities including progressive motility, sperm viability, sperm plasma membrane integrity and tail abnormalities were evaluated before dilution and after 4 hrs equilibration. It was found that the progressive motility of equilibrated semen in egg yolk and PC20 extenders were higher than those in PC95 extender （P 〈 0.05）. Sperm viability was lower in 1% and 2% PC95 extender compared to other extenders （P 〈 0.05）. PC20 extender maintained the sperm membrane integrity and normal tail morphology at low temperature better than egg yolk and PC95 （P 〈 0.05）. It can be concluded that 20% soybean phosphatidylcholine supplemented in TCF extender at 1%-3% （v/v） is as effective as hen egg yolk to preserve goat semen during equilibration at 4 ℃ for 4 hrs .

Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity

Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃.Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively.Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid (mHTF) (43.4%±7.9% vs 9.5%±2.5%, P<0.01 ).Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm (capacitated sperm: 7.6%±1.8% vs 6.4±1.8%; acrosome-reacted sperm: 3.0%±1.7% vs 2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (capacitated sperm: 16.0%±2.3% vs 7.6±1.8%, acrosome-reacted sperm: 9.4%±2.1% vs 3.0%±1.7%, P<0.01).The penetration rate and fertility index of cool-preserved human sperm in the EF solution were comparable with those of fresh sperm (48.1% vs 50.9%; 1.38±0.16 vs 1.29±0.13, respectively, P>0.05).Conclusion: Cold-preservation did not induce capacitation and acrosome reaction of human sperm in the EF solution, but human sperm cold-preserved in the EF solution for 1 week possesses as much penetration capacity as fresh sperm.

乌苏里拟鲿的人工繁殖技术总结

乌苏里拟鲿人工繁殖取得成功,并且取得创新型成果,催产率96%,受精率最高98.2%,平均93%,孵化率达75%,繁殖水花鱼苗30万尾;自主创新研制了拟鲿专用精子保存液,通过DNA断裂指数分析,建立了基于SCSA精子保存液使用效果的评价体系;拟鲿仿生态繁殖取得初步成功,孵化框和孵化槽推水装置有很大改进。