A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The ...A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells.Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.展开更多
The hierachical rank of Laurencia complex was changed recently and divided into three genera, Chondrophycus, Laurencia and Osmundea. This alga,Chondrophycus intermedius (Yamada) Garbary et Harper distributes mainly al...The hierachical rank of Laurencia complex was changed recently and divided into three genera, Chondrophycus, Laurencia and Osmundea. This alga,Chondrophycus intermedius (Yamada) Garbary et Harper distributes mainly along the coasts of East Asia PacifiC.The other distributions in other places are also given. In China, ever as Laurencia intermedia, it was described insufficiently, or reported erroneously or mixed up with Chondrophycus palisadus (Yamada) K.W. Nam (original L. palisada)and Chondrophycus capituliformis (Yamada) Garbary et Harper (original L. capituliformis). Some questions need to be clarified. Its morphology and anatomy from China coasts specimens are observed in detail with a lot of figures, and compared with other related species. As a distinct species, it differs from C.palisadus (Yamada) K.W. Nam and C.capituliformis (Yamada) Garbary et Harper in the physical characteristics such as sticking to the paper, the shape of cystocarp and the arrangement of superficial cells, and so on. Some questionable distributions are discussed.展开更多
基金This study was supported by grants from National Natural Science Foundation of China(No.30240019,30300060)National High Technology Research and Development Program of China(No.2001 AA221I31)+1 种基金Major State Basic Research Project(No.Gl 999055901)State Ministry of Science and Technology Program(No.2002BA7llA0l).
文摘A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells.Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
文摘The hierachical rank of Laurencia complex was changed recently and divided into three genera, Chondrophycus, Laurencia and Osmundea. This alga,Chondrophycus intermedius (Yamada) Garbary et Harper distributes mainly along the coasts of East Asia PacifiC.The other distributions in other places are also given. In China, ever as Laurencia intermedia, it was described insufficiently, or reported erroneously or mixed up with Chondrophycus palisadus (Yamada) K.W. Nam (original L. palisada)and Chondrophycus capituliformis (Yamada) Garbary et Harper (original L. capituliformis). Some questions need to be clarified. Its morphology and anatomy from China coasts specimens are observed in detail with a lot of figures, and compared with other related species. As a distinct species, it differs from C.palisadus (Yamada) K.W. Nam and C.capituliformis (Yamada) Garbary et Harper in the physical characteristics such as sticking to the paper, the shape of cystocarp and the arrangement of superficial cells, and so on. Some questionable distributions are discussed.
