本文应用微分干涉相衬法对优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl和暗褐蝈螽G.sedakovii(Fischer von Waldheim)雄性精巢管基部、输精管、贮精囊和精包,及雌性受精囊中精子束的形态变化进行了观察,对探讨螽斯近缘种的生...本文应用微分干涉相衬法对优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl和暗褐蝈螽G.sedakovii(Fischer von Waldheim)雄性精巢管基部、输精管、贮精囊和精包,及雌性受精囊中精子束的形态变化进行了观察,对探讨螽斯近缘种的生殖隔离机制和生殖生物学具有重要意义。结果表明:这两种蝈螽的精子束通过精包转移到雌性受精囊后,精子束的形态发生了显著变化。精巢管基部的精子为游离的单个精子;输精管、贮精囊和精包中精子成束排列形成较分散的精子束,精子束头部包裹有粘液帽;雌性受精囊中的精子束的精子呈羽状排列,精子的头部汇集在中央轴上。两种蝈螽精子束形态差异不显著。展开更多
为阐明优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl雄性附腺的结构与功能的关系,本文利用透射电镜(transmission electron microscope,TEM)技术研究了优雅蝈螽雄性附腺的超微结构,利用微分干涉相差显微镜(differential interfe...为阐明优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl雄性附腺的结构与功能的关系,本文利用透射电镜(transmission electron microscope,TEM)技术研究了优雅蝈螽雄性附腺的超微结构,利用微分干涉相差显微镜(differential interference contrast microscope,DIC)技术并结合雄性附腺匀浆提取物与精子束在体外的短暂培养,研究了优雅蝈螽雄性附腺对精子束的作用。结果表明:优雅蝈螽雄性附腺3类腺管组织结构相似,腺管管壁为单层上皮细胞,缺少内表皮,说明其来源于中胚层。上皮细胞富含粗面内质网、线粒体、高尔基体,具有分泌细胞的特点。腺管管腔中分泌物有4种形态,即电子透明的物质、电子致密的颗粒物质、细纤维状物质以及绒球状物质。上皮细胞的分泌方式主要有2种,即顶质分泌和局部分泌。乳白短腺管的匀浆提取物参与了帽状精子束解聚的过程,乳白长腺管和透明腺管的匀浆提取物有维持精子束活性的作用。本研究结果为进一步阐明螽斯雄性附腺的生理功能奠定了基础。展开更多
The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,st...The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,stitching and overlay of this system are evaluated.The system can write complex patterns at dimensions down to 30nm.The demonstrated overlay accuracy of this system is better than 40nm.展开更多
Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor...Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor location of the tumor with the possibility of estimating densities in the tissue surrounding the tumor to overcome dose calculation defects but radial estimated the patient.Despite the flaws associated with the systems and calculates the dose of your programs in all programs currently existing in the world.Than necessary,to the existence of a review of the accuracy of accounts and how to confirm the radiation dose to the patient programs.Methods A total of 35 cancer patients were considered for this study,with 245 field measurements made with low-and high-energy diode detectors for brain and prostate cases.The treatments for all patients were planned using Eclipse Treatment Planning System version 13.6.Results Of the 105 field measurements made for the prostate cancer patients,16 included discrepancies outside the ±5% action level.Of the 145 measurements taken of the brain cases,there were four outside the ±5% action level.The results indicated a higher degree of accuracy.The study revealed that,for the prostate measurements,the higher discrepancy in the doses for the particular fields(exceeding the action level) may have been due to the isocenter being very close to the jaws and multi-leaf collimator of the linear accelerator machine.As a result,scatter from the jaws and the multi-leaf collimator could have contributed to the high dose delivered to the diode;hence,a probable higher discrepancy of the dose in more brain cases due highest quality of VMAT technique and fixation system.Conclusion A greater percentage of the observed discrepancies were well within the set tolerance level.However,it is recommended that the positioning of the diode on the patient's skin and the angular sensitivity of the diodes be reconsidered.It is also recommended that a more accurate calculation of expected diode values be performed,especially for fields that pass through the table.These efforts would achieve action levels of ±5%.展开更多
文摘本文应用微分干涉相衬法对优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl和暗褐蝈螽G.sedakovii(Fischer von Waldheim)雄性精巢管基部、输精管、贮精囊和精包,及雌性受精囊中精子束的形态变化进行了观察,对探讨螽斯近缘种的生殖隔离机制和生殖生物学具有重要意义。结果表明:这两种蝈螽的精子束通过精包转移到雌性受精囊后,精子束的形态发生了显著变化。精巢管基部的精子为游离的单个精子;输精管、贮精囊和精包中精子成束排列形成较分散的精子束,精子束头部包裹有粘液帽;雌性受精囊中的精子束的精子呈羽状排列,精子的头部汇集在中央轴上。两种蝈螽精子束形态差异不显著。
文摘为阐明优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl雄性附腺的结构与功能的关系,本文利用透射电镜(transmission electron microscope,TEM)技术研究了优雅蝈螽雄性附腺的超微结构,利用微分干涉相差显微镜(differential interference contrast microscope,DIC)技术并结合雄性附腺匀浆提取物与精子束在体外的短暂培养,研究了优雅蝈螽雄性附腺对精子束的作用。结果表明:优雅蝈螽雄性附腺3类腺管组织结构相似,腺管管壁为单层上皮细胞,缺少内表皮,说明其来源于中胚层。上皮细胞富含粗面内质网、线粒体、高尔基体,具有分泌细胞的特点。腺管管腔中分泌物有4种形态,即电子透明的物质、电子致密的颗粒物质、细纤维状物质以及绒球状物质。上皮细胞的分泌方式主要有2种,即顶质分泌和局部分泌。乳白短腺管的匀浆提取物参与了帽状精子束解聚的过程,乳白长腺管和透明腺管的匀浆提取物有维持精子束活性的作用。本研究结果为进一步阐明螽斯雄性附腺的生理功能奠定了基础。
文摘The JEOL JBX-5000LS is a vector type machine.The system hardware features an ion-pumped column,a LaB 6 electron emitter,25kV and 50kV accelerating voltage,and a turbo-pumped sample chamber.The resolution,stability,stitching and overlay of this system are evaluated.The system can write complex patterns at dimensions down to 30nm.The demonstrated overlay accuracy of this system is better than 40nm.
文摘Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor location of the tumor with the possibility of estimating densities in the tissue surrounding the tumor to overcome dose calculation defects but radial estimated the patient.Despite the flaws associated with the systems and calculates the dose of your programs in all programs currently existing in the world.Than necessary,to the existence of a review of the accuracy of accounts and how to confirm the radiation dose to the patient programs.Methods A total of 35 cancer patients were considered for this study,with 245 field measurements made with low-and high-energy diode detectors for brain and prostate cases.The treatments for all patients were planned using Eclipse Treatment Planning System version 13.6.Results Of the 105 field measurements made for the prostate cancer patients,16 included discrepancies outside the ±5% action level.Of the 145 measurements taken of the brain cases,there were four outside the ±5% action level.The results indicated a higher degree of accuracy.The study revealed that,for the prostate measurements,the higher discrepancy in the doses for the particular fields(exceeding the action level) may have been due to the isocenter being very close to the jaws and multi-leaf collimator of the linear accelerator machine.As a result,scatter from the jaws and the multi-leaf collimator could have contributed to the high dose delivered to the diode;hence,a probable higher discrepancy of the dose in more brain cases due highest quality of VMAT technique and fixation system.Conclusion A greater percentage of the observed discrepancies were well within the set tolerance level.However,it is recommended that the positioning of the diode on the patient's skin and the angular sensitivity of the diodes be reconsidered.It is also recommended that a more accurate calculation of expected diode values be performed,especially for fields that pass through the table.These efforts would achieve action levels of ±5%.
