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正常生育力男性射出精子核解聚后组蛋白相对含量测定 被引量:1
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作者 薛同一 李建国 +1 位作者 江兰英 王一飞 《解剖学杂志》 CAS CSCD 北大核心 1992年第4期241-244,共4页
本实验应用显微分光光度计分析了经SDS——EDTA处理后的人精液标本,用苯胺蓝染色显示赖氨酸型组蛋白,计量核蛋白相时含量。结果表明正常生育力男性的精子经SDS-EDTA处理后,苯胺绿染色阳性的精子数增多,且染色强度亦增大。经SDS-EDTA解聚... 本实验应用显微分光光度计分析了经SDS——EDTA处理后的人精液标本,用苯胺蓝染色显示赖氨酸型组蛋白,计量核蛋白相时含量。结果表明正常生育力男性的精子经SDS-EDTA处理后,苯胺绿染色阳性的精子数增多,且染色强度亦增大。经SDS-EDTA解聚后,80%的苯胺蓝染色阳性精子核中的组蛋白相对含量均>2.3AU,而未经SDS-EDTA处理的样本中,苯胺蓝阳性精子的组蛋白相对含量均<2.3AU。故本实验阐明了精子核蛋白经解聚后再作组蛋白含量测定较为正确。 展开更多
关键词 人类精子 组蛋白 精子
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奶牛卵胞质内单精注射(ICSI)的研究 被引量:3
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作者 陈少轻 孙瑞珍 +2 位作者 吴江鸿 苏米娅 张家新 《安徽农业科学》 CAS 北大核心 2009年第2期599-601,615,共4页
[目的]探讨不同状态奶牛精子卵胞质内单精注射(ICSI)的效果。[方法]体外成熟培养24 h的奶牛卵母细胞注入不同状态精子,观察其体外发育情况。[结果]结果表明,试验1,研究精子用5 mmol DTT(二硫苏糖醇)预处理与精子不经过预处理的对照组的... [目的]探讨不同状态奶牛精子卵胞质内单精注射(ICSI)的效果。[方法]体外成熟培养24 h的奶牛卵母细胞注入不同状态精子,观察其体外发育情况。[结果]结果表明,试验1,研究精子用5 mmol DTT(二硫苏糖醇)预处理与精子不经过预处理的对照组的效果,经DTT处理后,精子解聚率和雄原核率显著高于对照组(30.4%和11.6%,47.8%和27.9%,P<0.05),两组的卵裂率差异不显著(85.0%和80.0%),囊胚率和孵化率显著高于对照组(45.8%和26.0%,31.3%和14.0%,P<0.05)。试验2,比较死、活精子的卵胞质内单精注射的效果,两者之间卵裂率(75.0%和79.4%)、囊胚率(27.9%和30.9%)和孵化率(13.2%和16.2%)均无显著性差异(P>0.05)。试验3,比较睾丸或附睾(头、体、尾)精子卵胞质内单精注射的效果,卵裂率(79.1%、81.8%、73.2%和75.0%)、囊胚率(23.3%、20.4%、19.5%和18.2%)和孵化率(14.0%、13.4%、12.2%和9.1%)均无显著性差异(P>0.05)。[结论]DTT预处理奶牛精子有助于提高ICSI的效率;死精子能用于奶牛卵母细胞的ICSI;从睾丸精子到附睾尾精子,虽其成熟程度有很大不同,但对ICSI受精不产生显著影响。 展开更多
关键词 奶牛 ICSI 精子解聚 雄原核 体外胚胎
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Photoassociation of Atomic BEC within Mean-Field Approximation: Exact Solutions
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作者 蔡卫 景辉 +1 位作者 詹明生 许京军 《Chinese Physics Letters》 SCIE CAS CSCD 2007年第4期867-870,共4页
We propose an exactly solvable method to study the coherent two-colour photoassociation of an atomic Bose- Einstein condensate, by linearizing the bilinear atom-molecule coupling, which allows us to conveniently probe... We propose an exactly solvable method to study the coherent two-colour photoassociation of an atomic Bose- Einstein condensate, by linearizing the bilinear atom-molecule coupling, which allows us to conveniently probe the quantum dynamics and statistics of the system. By preparing different initial states of the atomic condensate, we can observe very different quantum statistical properties of the system by exactly calculating the quadrature- squeezed and mode-correlated functions. 展开更多
关键词 BOSE-EINSTEIN CONDENSATE SUPERCHEMISTRY STATES
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In Vitro Antimicrobial Activity Study of Some New Arginine-based Biodegradable Poly (Ester Urethane)s and Poly (Ester Urea)s
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作者 Darejan Kharadze Tamar Memanishvili +4 位作者 Ketevan Mamulashvili Tina Omiadze Larisa Kirmelashvili Zaur Lomtatidze Ramaz Katsarava 《Journal of Chemistry and Chemical Engineering》 2015年第8期524-532,共9页
Bactericidal activity of some arginine based biodegradable polymers-PEURs (poly (ester urethane)s) and PEUs (poly (ester urea)s) with low cytotoxicity was studied in in vitro experiments. Various bacterial str... Bactericidal activity of some arginine based biodegradable polymers-PEURs (poly (ester urethane)s) and PEUs (poly (ester urea)s) with low cytotoxicity was studied in in vitro experiments. Various bacterial strains both Gram-positive and Gram-negative were used to explore the bactericidal activity of the cationic polymers. As the test objects, the following microorganisms were used: Bacillus subtilis, Staphylococcus aureus, Mycobacterium album, Pseudomonas fluorescens, Escherichia coli, Actinomyces griseus and Aspergillus niger. The obtained results showed that the new cationic polymers suppressed the growth of the studied microorganisms and the bactericidal activity of the tested cationic polymers strongly depending on their chemical structure. 展开更多
关键词 ARGININE cationic polymer poly (ester urethane) poly (ester urea) bactericidal activity
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