为了探讨毕赤酵母表达的重组人卵透明带ZP3蛋白(recombinant human zona pellucida-3 protein,rhZP3)及其多克隆抗体对小鼠和人精卵结合的影响,采用不同浓度的rhZP3以及空白培养液分别处理小鼠精子,然后再与小鼠卵子进行结合实验,观察...为了探讨毕赤酵母表达的重组人卵透明带ZP3蛋白(recombinant human zona pellucida-3 protein,rhZP3)及其多克隆抗体对小鼠和人精卵结合的影响,采用不同浓度的rhZP3以及空白培养液分别处理小鼠精子,然后再与小鼠卵子进行结合实验,观察经过不同处理的精子对透明带黏附及体外受精率的影响;用rhZP3以及空白培养液分别处理人精子,然后再与人卵子进行结合实验,观察经过不同处理的精子对透明带黏附的影响;用抗rhZP3抗体与阴性血清分别处理小鼠和人卵子,再与精子进行结合实验,观察多克隆抗体对精子粘附以及小鼠体外受精率的影响。实验结果表明,rhZP3和抗rhZP3多克隆抗体既能抑制人的体外精卵结合,也能抑制小鼠体外精卵结合,提示rhZP3具有天然透明带的特性,有发展成避孕疫苗和作为检测透明带抗体检测试剂的可能。展开更多
Objective: The hemizona assay(HZA) is an established functional test that examines in vitro sperm-zona pellucida binding capacity with high predictive power for fertilization outcome in IVF. The objective of this stud...Objective: The hemizona assay(HZA) is an established functional test that examines in vitro sperm-zona pellucida binding capacity with high predictive power for fertilization outcome in IVF. The objective of this study was to evaluate the value of the HZA as a predictor of pregnancy in patients undergoing controlled ovarian hyperstimulation(COH) and intrauterine insemination(IUI). Design: Prospective clinical study. Setting: Academic center. Patient(s): Eighty-two couples with unexplained or male factor infertility that underwent 313 IUI cycles. Intervention(s): Basic semen analysis and HZA were performed within three months of starting COH/IUI therapy. Main Outcome Measure(s): Hemizona index(HZI) and clinical pregnancy. Result(s): Overall, patients with an HZI of < 30 had a significantly lower pregnancy rate compared to patients with an HZI of ≥30(11.1%vs. 40.6%, respectively; P < .05; relative risk for failure to conceive: 1.5[confidence interval 1.2-1.9]). In all patients combined, and in the range of HZI 0-60, the duration of infertility(P=.000) and the HZI(P=.004) were significant determinants of conception(receiver operating characteristics(ROC) analysis). In couples with male infertility, the average path velocity and HZI were significant predictors of conception(P=.001 and P=.005, respectively, ROC analysis). The negative and positive predictive values of the HZA for pregnancy were 93%and 69%, respectively. Logistic regression analysis provided models of HZI(P=.021) and duration of infertility(P=.037) with highest predictability of conception in male factor and unexplained infertility groups, respectively. Conclusion(s): TheHZA predicted pregnancy in the IUI setting with high sensitivity and negative predictive value in couples withmale infertility. Results of this spermfunction test are useful in counseling couples before allocating them into COH/IUI therapy.展开更多
文摘为了探讨毕赤酵母表达的重组人卵透明带ZP3蛋白(recombinant human zona pellucida-3 protein,rhZP3)及其多克隆抗体对小鼠和人精卵结合的影响,采用不同浓度的rhZP3以及空白培养液分别处理小鼠精子,然后再与小鼠卵子进行结合实验,观察经过不同处理的精子对透明带黏附及体外受精率的影响;用rhZP3以及空白培养液分别处理人精子,然后再与人卵子进行结合实验,观察经过不同处理的精子对透明带黏附的影响;用抗rhZP3抗体与阴性血清分别处理小鼠和人卵子,再与精子进行结合实验,观察多克隆抗体对精子粘附以及小鼠体外受精率的影响。实验结果表明,rhZP3和抗rhZP3多克隆抗体既能抑制人的体外精卵结合,也能抑制小鼠体外精卵结合,提示rhZP3具有天然透明带的特性,有发展成避孕疫苗和作为检测透明带抗体检测试剂的可能。
文摘Objective: The hemizona assay(HZA) is an established functional test that examines in vitro sperm-zona pellucida binding capacity with high predictive power for fertilization outcome in IVF. The objective of this study was to evaluate the value of the HZA as a predictor of pregnancy in patients undergoing controlled ovarian hyperstimulation(COH) and intrauterine insemination(IUI). Design: Prospective clinical study. Setting: Academic center. Patient(s): Eighty-two couples with unexplained or male factor infertility that underwent 313 IUI cycles. Intervention(s): Basic semen analysis and HZA were performed within three months of starting COH/IUI therapy. Main Outcome Measure(s): Hemizona index(HZI) and clinical pregnancy. Result(s): Overall, patients with an HZI of < 30 had a significantly lower pregnancy rate compared to patients with an HZI of ≥30(11.1%vs. 40.6%, respectively; P < .05; relative risk for failure to conceive: 1.5[confidence interval 1.2-1.9]). In all patients combined, and in the range of HZI 0-60, the duration of infertility(P=.000) and the HZI(P=.004) were significant determinants of conception(receiver operating characteristics(ROC) analysis). In couples with male infertility, the average path velocity and HZI were significant predictors of conception(P=.001 and P=.005, respectively, ROC analysis). The negative and positive predictive values of the HZA for pregnancy were 93%and 69%, respectively. Logistic regression analysis provided models of HZI(P=.021) and duration of infertility(P=.037) with highest predictability of conception in male factor and unexplained infertility groups, respectively. Conclusion(s): TheHZA predicted pregnancy in the IUI setting with high sensitivity and negative predictive value in couples withmale infertility. Results of this spermfunction test are useful in counseling couples before allocating them into COH/IUI therapy.
