The studies from 7 or 8 days old mice were used to prepare the spermatogonial stem cells. The isolation and purification of spermatogonial stem cells were done by means of the Percoll discontinual density gradient con...The studies from 7 or 8 days old mice were used to prepare the spermatogonial stem cells. The isolation and purification of spermatogonial stem cells were done by means of the Percoll discontinual density gradient concentration. The cells of the 3rd band were collected and cultured in vitro in DMEM supplemented with 2.5% or 10% fetal bovine serum （FBS）. The results showed that with the feeder layer and 2.5% serum, the spermatogonial stem cells could proliferate, differentiate last more than 4 months.展开更多
Pesticide plays a vital role in agricultural, industrial and residemial pest control. In recent years the ‘pesticide problem＇ has been the focus of public interest because these chemicals have a very important role ...Pesticide plays a vital role in agricultural, industrial and residemial pest control. In recent years the ‘pesticide problem＇ has been the focus of public interest because these chemicals have a very important role in agriculture but these chemicals also act like endocrine disruptor. Experiments were carried out on 12 weeks old mice with 30 ± 2 gm. b.w. Endosulfan were administered at 3 mg/kg b.w daily by gavage method for five weeks followed by selenium treatment at 10 μg/kg.b.w./day for five weeks. 9 ＋ 2 arrangment were clearly observed in control group mice with well structured dynein and nexin arm while Endosulfan treated group show depolymerised microtubule and 9 ＋ 1 arrangment with degenerated mitochondria. While selenium treated group shows restoration of 9 ＋ 2 arrangement of microtubule. It is evident from study that endosulfan causes depolymerisation of microtubule while selenium administered group show normal polymerized microtubule and restores normal fertility in mice.展开更多
Porcine mesenchymal stem cells in postnatal muscle have been demonstrated to differentiate into adipocytes. This increases adipocyte number and lipid accumulation, and is thought to be the origin of intramuscular fat....Porcine mesenchymal stem cells in postnatal muscle have been demonstrated to differentiate into adipocytes. This increases adipocyte number and lipid accumulation, and is thought to be the origin of intramuscular fat. In this study, the effects of myostatin and arginine on adipogenic differentiation in mesenchymal stem cells derived from porcine muscle （pMDSCs） were investigated in vitro. Intracellular triglyceride levels were reduced by exogenous myostatin and increased by arginine supplementation or myostatin antibody （P〈0.01）. The inhibition of lipid accumulation by rnyostatin in pMDSCs was alleviated by arginine supplementation （P〈0.01）. Expression patterns of adipogenic transcription factors showed that exogenous myostatin suppressed PPAR72 and aP2 expression （P〈0.01）, while supplemental arginine or myostatin antibody promoted ADD1 expression （P〈0.01）. Furthermore, compared with the addition of either myostatin protein or antibody alone, ADD1 and PPARδ expression were promoted by the combination of arginine and myostatin （P〈0.01）, and arginine combined with myostatin antibody promoted the expression of ADD1, PPARδ, C/EBPα, PPARγ2 and LPL in pMDSCs （P〈0.05）. These results suggest that myostatin inhibits adipogenesis in pMDSCs, and that this can be alleviated by arginine supplementation, at least in part, through promoting ADD1 and PPARδ expression.展开更多
文摘The studies from 7 or 8 days old mice were used to prepare the spermatogonial stem cells. The isolation and purification of spermatogonial stem cells were done by means of the Percoll discontinual density gradient concentration. The cells of the 3rd band were collected and cultured in vitro in DMEM supplemented with 2.5% or 10% fetal bovine serum （FBS）. The results showed that with the feeder layer and 2.5% serum, the spermatogonial stem cells could proliferate, differentiate last more than 4 months.
文摘Pesticide plays a vital role in agricultural, industrial and residemial pest control. In recent years the ‘pesticide problem＇ has been the focus of public interest because these chemicals have a very important role in agriculture but these chemicals also act like endocrine disruptor. Experiments were carried out on 12 weeks old mice with 30 ± 2 gm. b.w. Endosulfan were administered at 3 mg/kg b.w daily by gavage method for five weeks followed by selenium treatment at 10 μg/kg.b.w./day for five weeks. 9 ＋ 2 arrangment were clearly observed in control group mice with well structured dynein and nexin arm while Endosulfan treated group show depolymerised microtubule and 9 ＋ 1 arrangment with degenerated mitochondria. While selenium treated group shows restoration of 9 ＋ 2 arrangement of microtubule. It is evident from study that endosulfan causes depolymerisation of microtubule while selenium administered group show normal polymerized microtubule and restores normal fertility in mice.
基金supported by the National Natural Science Foundation of China (Grant No.30972119)
文摘Porcine mesenchymal stem cells in postnatal muscle have been demonstrated to differentiate into adipocytes. This increases adipocyte number and lipid accumulation, and is thought to be the origin of intramuscular fat. In this study, the effects of myostatin and arginine on adipogenic differentiation in mesenchymal stem cells derived from porcine muscle （pMDSCs） were investigated in vitro. Intracellular triglyceride levels were reduced by exogenous myostatin and increased by arginine supplementation or myostatin antibody （P〈0.01）. The inhibition of lipid accumulation by rnyostatin in pMDSCs was alleviated by arginine supplementation （P〈0.01）. Expression patterns of adipogenic transcription factors showed that exogenous myostatin suppressed PPAR72 and aP2 expression （P〈0.01）, while supplemental arginine or myostatin antibody promoted ADD1 expression （P〈0.01）. Furthermore, compared with the addition of either myostatin protein or antibody alone, ADD1 and PPARδ expression were promoted by the combination of arginine and myostatin （P〈0.01）, and arginine combined with myostatin antibody promoted the expression of ADD1, PPARδ, C/EBPα, PPARγ2 and LPL in pMDSCs （P〈0.05）. These results suggest that myostatin inhibits adipogenesis in pMDSCs, and that this can be alleviated by arginine supplementation, at least in part, through promoting ADD1 and PPARδ expression.
