目的探讨精氨酸抗利尿激素(AVP)对急性肺损伤肺水肿液清除作用。方法 48只健康成年的雄性SD大鼠随机分为对照组、模型组(ALI组)、AVP组,观察各组肺组织病理形态学、肺水含量、肺泡上皮通透性及肺泡液体清除率(AFC)变化,测定肺泡上皮钠通...目的探讨精氨酸抗利尿激素(AVP)对急性肺损伤肺水肿液清除作用。方法 48只健康成年的雄性SD大鼠随机分为对照组、模型组(ALI组)、AVP组,观察各组肺组织病理形态学、肺水含量、肺泡上皮通透性及肺泡液体清除率(AFC)变化,测定肺泡上皮钠通道(ENa C)和钠/钾ATP酶(Na+,K+-ATPase)表达情况。结果经AVP治疗后,模型组肺泡上皮通透性(0.27±0.15 vs0.59±0.19)及肺水含量(5.01±1.59 vs 8.67±1.79)减轻,AFC增加(23.56±4.51 vs 8.28±3.57),α-ENa C(1.296±0.322 vs 0.349±0.141)和α1-Na+,K+-ATPase表达增加(1.421±0.389 vs 0.338±0.186),均有显著差异(P<0.05)。结论 AVP能促进AFC,其作用途径可能是上调α-ENa C和α1-Na+,K+-ATPase通道蛋白实现的。展开更多
Objective： To evaluate plasma arginine vasopressin （AVP） level in patients with traumatic brain injury and investigate the role of AVP in the process of brain edema. Methods ： A total of 30 patients with traumatic...Objective： To evaluate plasma arginine vasopressin （AVP） level in patients with traumatic brain injury and investigate the role of AVP in the process of brain edema. Methods ： A total of 30 patients with traumatic brain injury were involved in our study. They were divided into two groups by Glasgow Coma Scale： severe tranmatic brain injury group （ STBI, GCS ≤ 8 ） and moderate traumatic brain injury group （ MTBI, GCS 〉 8 ）. Samples of venous blood were collected in the morning at rest from 15 healthy volunteers （control group）and within 24 h after traumatic brain injury from these patients for AVP determinations by radioimmunoassay. The severity and duration of the brain edema were estimated by head CT scan. Results： plasma AVP levels （ng/L） were （mean± SD） control, 3.06 ± 1.49; MTBI, 38. 12 ± 7. 25; andSTBI, 66. 61 ± 17. 10. The plasma level of AVP was significantly increased within 24 h after traumatic brain injury and followed by the reduction of GCS, suggesting the deterioration of cerebral injury （ P 〈 0.01 ）. And the AVP level was correlated with the severity （ STBI r = 0. 919, P 〈 0.01 ; MTBI r = 0. 724, P 〈 0.01 ） and the duration of brain edema （STBI r =0.790, P 〈0.01; MTBI r =0.712, P〈0.01）. Conclusions. The plasma AVP level is dosely associated with the severity of traumatic brain injury. AVP may play an important role in pathogenesis of brain edema after traumatic brain injury.展开更多
文摘目的探讨精氨酸抗利尿激素(AVP)对急性肺损伤肺水肿液清除作用。方法 48只健康成年的雄性SD大鼠随机分为对照组、模型组(ALI组)、AVP组,观察各组肺组织病理形态学、肺水含量、肺泡上皮通透性及肺泡液体清除率(AFC)变化,测定肺泡上皮钠通道(ENa C)和钠/钾ATP酶(Na+,K+-ATPase)表达情况。结果经AVP治疗后,模型组肺泡上皮通透性(0.27±0.15 vs0.59±0.19)及肺水含量(5.01±1.59 vs 8.67±1.79)减轻,AFC增加(23.56±4.51 vs 8.28±3.57),α-ENa C(1.296±0.322 vs 0.349±0.141)和α1-Na+,K+-ATPase表达增加(1.421±0.389 vs 0.338±0.186),均有显著差异(P<0.05)。结论 AVP能促进AFC,其作用途径可能是上调α-ENa C和α1-Na+,K+-ATPase通道蛋白实现的。
文摘Objective： To evaluate plasma arginine vasopressin （AVP） level in patients with traumatic brain injury and investigate the role of AVP in the process of brain edema. Methods ： A total of 30 patients with traumatic brain injury were involved in our study. They were divided into two groups by Glasgow Coma Scale： severe tranmatic brain injury group （ STBI, GCS ≤ 8 ） and moderate traumatic brain injury group （ MTBI, GCS 〉 8 ）. Samples of venous blood were collected in the morning at rest from 15 healthy volunteers （control group）and within 24 h after traumatic brain injury from these patients for AVP determinations by radioimmunoassay. The severity and duration of the brain edema were estimated by head CT scan. Results： plasma AVP levels （ng/L） were （mean± SD） control, 3.06 ± 1.49; MTBI, 38. 12 ± 7. 25; andSTBI, 66. 61 ± 17. 10. The plasma level of AVP was significantly increased within 24 h after traumatic brain injury and followed by the reduction of GCS, suggesting the deterioration of cerebral injury （ P 〈 0.01 ）. And the AVP level was correlated with the severity （ STBI r = 0. 919, P 〈 0.01 ; MTBI r = 0. 724, P 〈 0.01 ） and the duration of brain edema （STBI r =0.790, P 〈0.01; MTBI r =0.712, P〈0.01）. Conclusions. The plasma AVP level is dosely associated with the severity of traumatic brain injury. AVP may play an important role in pathogenesis of brain edema after traumatic brain injury.
