Three steam distillation devices (D-1, D-2 and D-4) or one simultaneous distillation (D-3, water-diethyl ether) as well as the process of CO2-SFE (Supercritical fluid extraction) were adopted in extraction of essentia...Three steam distillation devices (D-1, D-2 and D-4) or one simultaneous distillation (D-3, water-diethyl ether) as well as the process of CO2-SFE (Supercritical fluid extraction) were adopted in extraction of essential oils from Chinese-fir (Cunninghamia lancedata (Lamb) Hook.) and the chemical components of the extracted essential oil were analyzed by Gas chromatograph-MS analyses. The results showed that the essential oil could be almost extracted out within 2 hours and the device-3 had the highest extraction efficiency. The major chemical component of the oil was cedrol. The yield of the extracted essential oils from Chinese fir decreased gradually with the increase of the distillation time. The best condition for extraction by means of CO2-SFE is 100 kg·cm?2 in pressure and 40°C in temperature for. Keywords Chinese fir - Essential oil - Cedrol - Supercritical fluid extraction CLC number S781.4 Document code A Foundation item: This paper was support by the Key Foundation Research Project (G1999016001) of China and the Japan International Cooperation AgencyBiography: HUANG Luo-hua (1957-), male, Research associate, Research Institute of Wood Industry, Chinese Academy of forestry, Beijing 100091, P. R. ChinaResponsible editor: Song Funan展开更多
This study aimed to model the kinetic of hydro-distillation of Aquilaria malaccensis leaves oil in order to understand and optimize the extraction process. In addition, this study, for the first time, aimed to identif...This study aimed to model the kinetic of hydro-distillation of Aquilaria malaccensis leaves oil in order to understand and optimize the extraction process. In addition, this study, for the first time, aimed to identify the chemical compositions of the A. rnalaccensis leave-oil. By assessing both first-order kinetic model and the model of simultaneous washing and diffusion, the result indicated that the model of simultaneous washing and diffusion better describes the hydro-distillation mechanism of the essential oil from A. rnalaccensis leaves. The optimum time, solid to liquid ratio, and the heating power for extracting the highest amount of essential oil were found to be around 3 h, 1：10 （g. ml-1）, and 300 W respectively. Yellow essential oil with a strong smell and a yield of 0.05 v/w was extracted by hydro-distillation Clevenger apparatus. Chemical compounds of the essential oil were analyzed using gas chromatography-mass spectroscopy （GC/MS）, which resulted in identification of 42 compounds that constitute 93% of essential oil. Among the identified components, Pentadecanal （32.082%）, 9-Octadecenal, （Z） （15.894%）, and Tetradecanal （6.927%） were the major compounds. Considering the fact that all the identified major components possess pesticidal properties, A. malaccensis leaves can be regarded as a promising natural source for producing pesticides.展开更多
OBJECTIVE: To analyze the essential oils from flowers, leaves, stems, roots, and fruits of Chaihu(Radix Bupleuri Chinensis).METHODS: We extracted essential oils from different parts of Chaihu(Radix Bupleuri Chinensis)...OBJECTIVE: To analyze the essential oils from flowers, leaves, stems, roots, and fruits of Chaihu(Radix Bupleuri Chinensis).METHODS: We extracted essential oils from different parts of Chaihu(Radix Bupleuri Chinensis) using a steam distillation method. The essential oils were analyzed by gas chromatography-mass spectrometry(GC-MS). Data were collected in full scan mode(m/z 60-600). Volatile components were identified based on their retention indices and by comparing their mass spectra with those in the National Institute of Standards and Technology 2005 database assisted by tandem mass spectrometry information. The relative content of each constituent wasdetermined by area normalization.RESULTS: We identified 111 components, of which12 were common to all 5 parts, 30 were found only in roots, 14 were found only in flowers, 6 were found only in leaves, 4 were found only in stems,and 17 were found only in fruits.CONCLUSION: Our results show that the stems,flowers, leaves, and fruits of Chaihu(Radix Bupleuri Chinensis)contain a high concentration of essential oils, and that the exact composition of the essential oils differs among the plant parts. To develop new medicines and make full use of the Chaihu(Radix Bupleuri Chinensis)resource, it is important to characterize the essential oils from different parts of the plant. In future research, it will be important to determine the pharmacological effects of the various components and the essential oil mixtures.展开更多
基金The Key Foundation Research Project (G1999016001) of China and the Japan International Cooperation Agency
文摘Three steam distillation devices (D-1, D-2 and D-4) or one simultaneous distillation (D-3, water-diethyl ether) as well as the process of CO2-SFE (Supercritical fluid extraction) were adopted in extraction of essential oils from Chinese-fir (Cunninghamia lancedata (Lamb) Hook.) and the chemical components of the extracted essential oil were analyzed by Gas chromatograph-MS analyses. The results showed that the essential oil could be almost extracted out within 2 hours and the device-3 had the highest extraction efficiency. The major chemical component of the oil was cedrol. The yield of the extracted essential oils from Chinese fir decreased gradually with the increase of the distillation time. The best condition for extraction by means of CO2-SFE is 100 kg·cm?2 in pressure and 40°C in temperature for. Keywords Chinese fir - Essential oil - Cedrol - Supercritical fluid extraction CLC number S781.4 Document code A Foundation item: This paper was support by the Key Foundation Research Project (G1999016001) of China and the Japan International Cooperation AgencyBiography: HUANG Luo-hua (1957-), male, Research associate, Research Institute of Wood Industry, Chinese Academy of forestry, Beijing 100091, P. R. ChinaResponsible editor: Song Funan
文摘This study aimed to model the kinetic of hydro-distillation of Aquilaria malaccensis leaves oil in order to understand and optimize the extraction process. In addition, this study, for the first time, aimed to identify the chemical compositions of the A. rnalaccensis leave-oil. By assessing both first-order kinetic model and the model of simultaneous washing and diffusion, the result indicated that the model of simultaneous washing and diffusion better describes the hydro-distillation mechanism of the essential oil from A. rnalaccensis leaves. The optimum time, solid to liquid ratio, and the heating power for extracting the highest amount of essential oil were found to be around 3 h, 1：10 （g. ml-1）, and 300 W respectively. Yellow essential oil with a strong smell and a yield of 0.05 v/w was extracted by hydro-distillation Clevenger apparatus. Chemical compounds of the essential oil were analyzed using gas chromatography-mass spectroscopy （GC/MS）, which resulted in identification of 42 compounds that constitute 93% of essential oil. Among the identified components, Pentadecanal （32.082%）, 9-Octadecenal, （Z） （15.894%）, and Tetradecanal （6.927%） were the major compounds. Considering the fact that all the identified major components possess pesticidal properties, A. malaccensis leaves can be regarded as a promising natural source for producing pesticides.
基金Breeding Research Project of Chinese Medical Herbs in Sichuan Province:Study on the breeding of new Chaihu variety and cultivation and the related techniques(No.2011NZ0098-12-11)China Spark Program in 2010(No.2010GA810056)
文摘OBJECTIVE: To analyze the essential oils from flowers, leaves, stems, roots, and fruits of Chaihu(Radix Bupleuri Chinensis).METHODS: We extracted essential oils from different parts of Chaihu(Radix Bupleuri Chinensis) using a steam distillation method. The essential oils were analyzed by gas chromatography-mass spectrometry(GC-MS). Data were collected in full scan mode(m/z 60-600). Volatile components were identified based on their retention indices and by comparing their mass spectra with those in the National Institute of Standards and Technology 2005 database assisted by tandem mass spectrometry information. The relative content of each constituent wasdetermined by area normalization.RESULTS: We identified 111 components, of which12 were common to all 5 parts, 30 were found only in roots, 14 were found only in flowers, 6 were found only in leaves, 4 were found only in stems,and 17 were found only in fruits.CONCLUSION: Our results show that the stems,flowers, leaves, and fruits of Chaihu(Radix Bupleuri Chinensis)contain a high concentration of essential oils, and that the exact composition of the essential oils differs among the plant parts. To develop new medicines and make full use of the Chaihu(Radix Bupleuri Chinensis)resource, it is important to characterize the essential oils from different parts of the plant. In future research, it will be important to determine the pharmacological effects of the various components and the essential oil mixtures.
