Mito-tempo对绵羊精液低温保存效果的影响

实验旨在探究不同浓度的Mito-tempo对绵羊精液低温保存效果的影响。采集8只湖羊种公羊精液,分别用含0、15、30、45、60μmol/L Mito-tempo稀释液进行8倍稀释,5℃冷藏120 h。分别在24 h和120 h检测精液品质指标(活力、活率、平均路径速度、曲线运动速度、直线运动速度、质膜完整性、顶体完整性、DNA完整性和线粒体活性)及抗氧化指标(总抗氧化能力、超氧化物歧化酶活性和丙二醛含量)。结果表明:在低温保存期间,30μmol/L Mito-tempo处理组精子活力、活率、平均路径速度、DNA完整性、顶体完整性、线粒体活性、超氧化物歧化酶含量和总抗氧化能力均高于对照组及60μmol/L Mito-tempo处理组(P<0.05);在低温保存过程中,30μmol/L Mito-tempo处理组丙二醛含量低于对照组和其余各试验组(P<0.05)。由此可见,在稀释液中添加30μmol/L Mito-tempo可有效提高低温保存后绵羊精液质量。

蜂王浆对低温保存绵羊精子质量和糖转运蛋白GLUTs表达的影响

实验旨在研究稀释液中添加蜂王浆对低温保存绵羊精子质量和糖转运蛋白丰度的影响。通过假阴道法采集8只杜泊公羊精液,将活率不低于0.8的精液混合,分别用含0.00%、0.25%、0.50%、0.75%和1.00%蜂王浆的稀释液将精液稀释至8倍,0.00%蜂王浆组为对照组,置于5℃保存。在保存0、24、48、72 h利用计算机辅助精子分析系统、低渗膨胀实验、双荧光探针和Western Blot分别检测精子运动参数、质膜完整性、线粒体活性以及糖转运蛋白GLUT 3和GLUT 8丰度。结果表明:在低温保存72 h时,0.75%蜂王浆组精子的活率、活力、直线运动速率、路径速度、曲线运动速率、质膜完整性和线粒体活性均显著高于对照组。Western Blot结果显示精子GLUT 3和GLUT 8丰度均随保存时间延长而降低,保存72 h时GLUT 8的丰度显著低于0 h;保存72 h时0.75%蜂王浆组GLUT 3和GLUT 8丰度显著高于对照组。综上,在稀释液中添加0.75%的蜂王浆可有效提高低温保存绵羊精子的质量,这与精子GLUTs蛋白表达和精子能量代谢密切相关。

低温保存对绵羊精子运动参数与线粒体活性和抗氧化水平的影响

低温造成的氧化损伤是精液品质下降的主要原因。本试验旨在研究4℃低温保存对绵羊精子运动参数、线粒体活性和抗氧化水平的影响。采集3只杜泊公羊的精液,使用常规卵黄稀释液进行8倍稀释后放入4℃冰箱保存,分别在0 h、24 h、48 h和72 h后检测精子运动参数和抗氧化水平。结果表明,随着保存时间的延长,精子活率和运动参数逐渐下降,但72 h后精子活力仍保持在50%左右,活率高于70%;GSH-Px酶活性和T-AOC逐渐降低,MDA逐渐升高,72 h后精子线粒体活性显著低于0 h,但与48 h相比差异不显著。研究表明,精液在低温保存72 h后,其质量、抗氧化水平和线粒体活性均会显著下降,可以考虑在这个时间段加入线粒体靶向抗氧化剂,以延长精子的保存时间,减缓精液品质下降程度。

Effects of Magnetic Field on the Quality of Chicken Semen Stored at Low Temperature

[ Objective] The study aimed to explore the effects of magnetic treatment on the quality of chicken semen stored at low temperature. [ Method] 5 ml fresh chicken semen was divided into five groups equally, each of which was diluted at the volume ratio 1：4. With the group without magnetic treatment as the control, the other four groups were magnetized for 6, 12, 24 and 48 min in the self-made magnetizer, respectively. Subsequently, all the five groups were stored at 2 -4 ℃, and the sperm motility, survival time, survival index and deformity rate were observed regularly. [ Result] Comparing with the control group, the magnetic groups showed higher sperm motilities and effective survival indices as well as lower deformity rates. The effective survival index of the group magnetized for 24 min was the highest and increased by 7.75% in con- trast to the control. [ Conclusion] Magnetic treatment can effectively enhance the quality of chicken semen stored at low temperature.