A total of 18 ejaculates were collected, once per week, from six fertile stallions for three consecutive weeks in October and November, to compare motility over time between extenders using four semen processing treat...A total of 18 ejaculates were collected, once per week, from six fertile stallions for three consecutive weeks in October and November, to compare motility over time between extenders using four semen processing treatments. Four total aliquots of semen were used. Two aliquots of each semen collection were extended in either INRA96 or an experimental proprietary milk-based extender Walworth （WW） extender, and each was designed for multi-day storage of fresh chilled semen. Each aliquot was divided again, and either centrifuged at 600 μg for 10 min without cushion, or not centrifuged and extended to a final concentration of 25 × 10^6 spermatozoa/mL. The treatments evaluated were INRA96 without centrifugation （INRA-NC） or with centrifugation （INRA-C）, and Walworth extender without centrifugation （WW-NC） or with centrifugation （WW-C）. Total and progressive motility were measured using Sperm Vision~ CASA at 0, 24, 48 and 72 h post-collection. Samples were stored at 4 ℃. No differences were found between extenders in progressive （P = 0.13） or total motility （P = 0.14） over the four different time points without centrifugation. However, ejaculates processed in INRA-C group had the greater total and progressive motility （P 〈 0.05） over the four time points than ejaculates in the WW-C group. It was found that centrifugation and re-suspension of stallion semen in INRA96 improved the longevity of fresh chilled semen. However, when not using centrifugation, the Walworth extender proved to be as effective for maintaining spermatozoa motility across all time points as 1NRA96 , and may be an alternative for use in the equine breeding industry.展开更多
Due to the small volume of goat semen ejaculate, just a few doses of goat semen were produced when the sperm concentration is 100 × 10^/mL. The study was aimed to determine the viability of extended goat semen at...Due to the small volume of goat semen ejaculate, just a few doses of goat semen were produced when the sperm concentration is 100 × 10^/mL. The study was aimed to determine the viability of extended goat semen at refrigerated condition at 5 ℃ using varying sperm concentrations and evaluated if sperm concentration lower than 100 × 10^6/mL would affect the motility, viability and sperm morphology at refrigerated condition. Using an artificial vagina, ejaculated goat semen was collected from goat semen donor aged 1.5 year. Physical evaluation of the collected semen showed an average volume of 0.54 mL, mean pH of 6.8 and a milky white color with thick consistency indicative of high concentration. Fresh goat semen had an initial average of 76% with an average initial sperm concentration of 128 × 10^/mL. The semen was divided into four treatments： sperm concentration of 100× 10^/mL, 75 × 10^/mL, 50 × 10^/mL and 25 × 10^/mL, and were stored at 5 ℃ for a period of 10 d. The semen evaluation was performed for each of the four treatments every other day. Results showed that the sperm concentration of spermatozoa affected the duration of storage based on the sperm motility percentage, viability and morphology of spermatozoa. The extended goat semen with sperm concentration of 25 and 50 million sperm/mL is optimal for storage within 6 d that gave satisfactory percentage motile, viable and morphologically normal spermatozoa.展开更多
文摘A total of 18 ejaculates were collected, once per week, from six fertile stallions for three consecutive weeks in October and November, to compare motility over time between extenders using four semen processing treatments. Four total aliquots of semen were used. Two aliquots of each semen collection were extended in either INRA96 or an experimental proprietary milk-based extender Walworth （WW） extender, and each was designed for multi-day storage of fresh chilled semen. Each aliquot was divided again, and either centrifuged at 600 μg for 10 min without cushion, or not centrifuged and extended to a final concentration of 25 × 10^6 spermatozoa/mL. The treatments evaluated were INRA96 without centrifugation （INRA-NC） or with centrifugation （INRA-C）, and Walworth extender without centrifugation （WW-NC） or with centrifugation （WW-C）. Total and progressive motility were measured using Sperm Vision~ CASA at 0, 24, 48 and 72 h post-collection. Samples were stored at 4 ℃. No differences were found between extenders in progressive （P = 0.13） or total motility （P = 0.14） over the four different time points without centrifugation. However, ejaculates processed in INRA-C group had the greater total and progressive motility （P 〈 0.05） over the four time points than ejaculates in the WW-C group. It was found that centrifugation and re-suspension of stallion semen in INRA96 improved the longevity of fresh chilled semen. However, when not using centrifugation, the Walworth extender proved to be as effective for maintaining spermatozoa motility across all time points as 1NRA96 , and may be an alternative for use in the equine breeding industry.
文摘Due to the small volume of goat semen ejaculate, just a few doses of goat semen were produced when the sperm concentration is 100 × 10^/mL. The study was aimed to determine the viability of extended goat semen at refrigerated condition at 5 ℃ using varying sperm concentrations and evaluated if sperm concentration lower than 100 × 10^6/mL would affect the motility, viability and sperm morphology at refrigerated condition. Using an artificial vagina, ejaculated goat semen was collected from goat semen donor aged 1.5 year. Physical evaluation of the collected semen showed an average volume of 0.54 mL, mean pH of 6.8 and a milky white color with thick consistency indicative of high concentration. Fresh goat semen had an initial average of 76% with an average initial sperm concentration of 128 × 10^/mL. The semen was divided into four treatments： sperm concentration of 100× 10^/mL, 75 × 10^/mL, 50 × 10^/mL and 25 × 10^/mL, and were stored at 5 ℃ for a period of 10 d. The semen evaluation was performed for each of the four treatments every other day. Results showed that the sperm concentration of spermatozoa affected the duration of storage based on the sperm motility percentage, viability and morphology of spermatozoa. The extended goat semen with sperm concentration of 25 and 50 million sperm/mL is optimal for storage within 6 d that gave satisfactory percentage motile, viable and morphologically normal spermatozoa.
