Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different...Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different boar breeds,the sperm motility,sperm viability and sperm deformity,as well as the spermadhesins＇ m RNA expression in fresh semen of Luchuan,Landrace and Duroc boars were compared.The results showed that the sperm motility and viability of Luchuan boar showed no significant differences with those of Landrace and Duroc boars（P〉 0.05）,but its sperm deformity was significantly lower than those of the other two exotic breeds（P〈0.01）;no significant differences were found in the m RNA expression levels of spermadhesins between Luchuan and Landrace,Duroc boars（P〉0.05）,excepting that the m RNA expression level of AQN1 in Luchuan boar semen was significantly lower than that in Duroc boar semen（P〈0.05）;the m RNA expression levels of AQN3,AWN and PSP-II in Landrace boar semen were significantly higher than those in Duroc boar semen（P〈0.05,P〈0.01）.In short,there were significant differences in the m RNA expression levels of spermadhesins among different pig breeds,which showed no significant correlations with the differences in sperm motility,viability and deformity.展开更多
The asialoglycoprotein （ASGP） receptor is a wellcharacterized hepatic receptor that is recycled via the common cellular process of receptor-mediated endocytosis （RME）. The RME process plays an integral part in the...The asialoglycoprotein （ASGP） receptor is a wellcharacterized hepatic receptor that is recycled via the common cellular process of receptor-mediated endocytosis （RME）. The RME process plays an integral part in the proper trafficking and routing of receptors and ligands in the healthy cell. Thus, the missorting or altered transport of proteins during RME is thought to play a role in several diseases associated with hepatocyte and liver dysfunction. Previously, we examined in detail alterations that occur in hepatocellular RME and associated receptor functions as a result of one particular liver injury, alcoholic liver disease （ALD）. The studies revealed profound ethanol- mediated impairments to the ASGP receptor and the RME process, indicating the importance of this receptor and the maintenance of proper endocytic events in normal tissue. To further clarify these observations, studies were performed utilizing knockout mice （lacking a functional ASGP receptor） to which were administered several liver toxicants. In addition to alcohol, we examined the effects following administration of anti- Fas （CD95） antibody, carbon tetrachloride （CCh） and lipopolysaccharide （LPS）/galactosamine. The results of these studies demonstrated that the knockout mice sustained enhanced liver injury in response to all of the treatments, as shown by increased indices of liver damage, such as enhancement of serum enzyme levels, histopathological scores, as well as hepatocellular death. Overall, the work completed to date suggests a possible link between hepatic receptors and liver injury. In particular, adequate function and content of the ASGP receptor may provide protection against various toxinmediated liver diseases.展开更多
文摘Spermadhesins,as the most important components of boar semen seminoproteins,play a regulatory role in sperm motility,sperm capacitation and sperm-ovum fusion.In order to study the fertility differences among different boar breeds,the sperm motility,sperm viability and sperm deformity,as well as the spermadhesins＇ m RNA expression in fresh semen of Luchuan,Landrace and Duroc boars were compared.The results showed that the sperm motility and viability of Luchuan boar showed no significant differences with those of Landrace and Duroc boars（P〉 0.05）,but its sperm deformity was significantly lower than those of the other two exotic breeds（P〈0.01）;no significant differences were found in the m RNA expression levels of spermadhesins between Luchuan and Landrace,Duroc boars（P〉0.05）,excepting that the m RNA expression level of AQN1 in Luchuan boar semen was significantly lower than that in Duroc boar semen（P〈0.05）;the m RNA expression levels of AQN3,AWN and PSP-II in Landrace boar semen were significantly higher than those in Duroc boar semen（P〈0.05,P〈0.01）.In short,there were significant differences in the m RNA expression levels of spermadhesins among different pig breeds,which showed no significant correlations with the differences in sperm motility,viability and deformity.
基金Supported by The National Institute on Alcohol Abuse and Alcoholismthe Department of Veterans Affairs
文摘The asialoglycoprotein （ASGP） receptor is a wellcharacterized hepatic receptor that is recycled via the common cellular process of receptor-mediated endocytosis （RME）. The RME process plays an integral part in the proper trafficking and routing of receptors and ligands in the healthy cell. Thus, the missorting or altered transport of proteins during RME is thought to play a role in several diseases associated with hepatocyte and liver dysfunction. Previously, we examined in detail alterations that occur in hepatocellular RME and associated receptor functions as a result of one particular liver injury, alcoholic liver disease （ALD）. The studies revealed profound ethanol- mediated impairments to the ASGP receptor and the RME process, indicating the importance of this receptor and the maintenance of proper endocytic events in normal tissue. To further clarify these observations, studies were performed utilizing knockout mice （lacking a functional ASGP receptor） to which were administered several liver toxicants. In addition to alcohol, we examined the effects following administration of anti- Fas （CD95） antibody, carbon tetrachloride （CCh） and lipopolysaccharide （LPS）/galactosamine. The results of these studies demonstrated that the knockout mice sustained enhanced liver injury in response to all of the treatments, as shown by increased indices of liver damage, such as enhancement of serum enzyme levels, histopathological scores, as well as hepatocellular death. Overall, the work completed to date suggests a possible link between hepatic receptors and liver injury. In particular, adequate function and content of the ASGP receptor may provide protection against various toxinmediated liver diseases.
