A major goal of research in biotechnology and nanotechnology is to develop assemblies of novel biomaterials that can be used in analytical, industrial, and therapeutic applications. The synthesis of DNA （oligodeoxyri...A major goal of research in biotechnology and nanotechnology is to develop assemblies of novel biomaterials that can be used in analytical, industrial, and therapeutic applications. The synthesis of DNA （oligodeoxyribonucleotide） has been described containing the biaryl-type nucleoside surrogates, 6-deamino-2＇-deoxy-6-（l-naphthyl） adenosine （AN） and 6-deamino-2＇-deoxy-6-（1-pyrenyl） adenosine （AP）. It is found that incorporation of multiple ATM or AP residues in the middle of DNA duplexes does not significantly destabilize the duplexes and that the fluorescence intensities of the oligonucleotides containing AN or Ap significantly increases in the duplexes formed.展开更多
文摘A major goal of research in biotechnology and nanotechnology is to develop assemblies of novel biomaterials that can be used in analytical, industrial, and therapeutic applications. The synthesis of DNA （oligodeoxyribonucleotide） has been described containing the biaryl-type nucleoside surrogates, 6-deamino-2＇-deoxy-6-（l-naphthyl） adenosine （AN） and 6-deamino-2＇-deoxy-6-（1-pyrenyl） adenosine （AP）. It is found that incorporation of multiple ATM or AP residues in the middle of DNA duplexes does not significantly destabilize the duplexes and that the fluorescence intensities of the oligonucleotides containing AN or Ap significantly increases in the duplexes formed.
