[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before head...[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant.展开更多
In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statisti...In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS (2 250 g/hm^2), 46% Prochloraz-Chitosan EW (450 g/hm^2), 10% difenoconazole WG (900 g/hm^2, CK) or 24% thifluzamide SC (360 ml/hm^2) were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of 'modern agriculture, green agriculture, organic agriculture and ecological agriculture.展开更多
To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to c...To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan + Gibberellin-heteroauxin-brassinolide (GHB) and Ningnanmycin + GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml + GHB WP 600 g and 8% Ningnanmycin AS 900 ml + GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride (CK) + GHB. The obtained results indicated that Lentinan + GHB and Ningnanmycin + GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production.展开更多
Aim To investigate the effects of milrinone (a selective phosphodiesteraseIII inhibitor PDE_3 ) on insulin secretion, blood glucose, plasma free fatty acids (FFA) anddose-response relationship, and assess possible eff...Aim To investigate the effects of milrinone (a selective phosphodiesteraseIII inhibitor PDE_3 ) on insulin secretion, blood glucose, plasma free fatty acids (FFA) anddose-response relationship, and assess possible effects of milrinone on glucose metabolism andinsulin sensitivity in conscious rats. Methods The catheterized nonstressed rats were administeredvarious doses of milrinone (1, 5, 25μmoL·kg^(-1)) and were compared with controls. Ahyperinsulinaemic-eugly-caemic clamp was established in counscious rats, andmilrinone(25μmoL·kg^(-1)) and 25% dimethyl sulfoxide (DMSO, as a control) were given at 120 minduring hyperinsulinaemic-euglycaemic clamping. Glucose turnover was determind with by gaschromatograph mass spectrometer (GC-MS). Results After dosing, plasma FFA levels in 3 milrinonegroups significantly increased, compared with the controls and before dosing. The percentages ofelevation of FFA by the different milrinone doses were very similar, 50%, and 52% , 55% for 1, 5,and 25 μmoL·kg^(-1), repectively, at 2 min after dosing. Plasma insulin levels were significantlyelevated in the 5 and 25 μmoL·kg^(-1) groups, and the effect of milrione on glucose concentrationwas detectable only in 25μmoL·kg^(-1) group. During hyperinsulinaemic clamping, there weresignificant increase, in plasma FFA (from 173 +- 15 to 634 +- 87μmoL·kg^(-1)) and hepatic glucoseproduction (HGP), and a significant decrease in glucose infusion rates (GIR) to about 21% and aslight increase in plasma insulin after milrinone treatment. Conclusion Milrinone impaires theability of insulin to suppress lipolysis and HGP, and insulin-mediated glucose utilization inperipheral tissue. Therefore, milrinone administration may induce an acute insulin resistance invivo.展开更多
A general situation of the sugar beet industry and business in the European Union(EU) has been presented. It included the quotas of sugar production there, its prices and changes on the market. The latest sugar produc...A general situation of the sugar beet industry and business in the European Union(EU) has been presented. It included the quotas of sugar production there, its prices and changes on the market. The latest sugar production data in the world was also given. The main companies carrying out activities on the Polish sugar and sugar beet seeds were also presented.New circumstances after liberating the sugar market in the EU in October, 2017, were also presented. Nowadays, Poland has been a great potential country for further technological progress in agricultural holdings and growing performance in sugar processing and production industry. As food export from Poland increases, it will be followed by increasing use of sugar. Examples are given to improve sugar beet productivity.展开更多
The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water ...The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water (WtF) from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence (CL) assay and hydroxyl free radical-initiated chemiluminescence (CL) assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.展开更多
Organophosphorous pesticide(OP) contamination has serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populate...Organophosphorous pesticide(OP) contamination has serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA(ss DNA)-wrapped gold nanoparticles(Au NPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer(OBA) as the recognition element, aptamer-wrapped Au NPs(Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from Au NPs and the aggregation of Au NPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of Au NPs, the established assay showed good linearity between 0.1 and 10 μmol/L, with a low detection limit of 0.1 μmol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.展开更多
基金Supported by Special Program for Technology Innovation from Yibin Municipal Bureau of Science(No.200905105)~~
文摘[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant.
基金Supported by Circular of the Ministry of Agriculture on Approval of the First Batch of the National Demonstration Zones for Modern Agriculture(NJF[2010]22)Fundamental Research Funds for the Central Universities(XDJK2015C057,SWU114046)~~
文摘In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS (2 250 g/hm^2), 46% Prochloraz-Chitosan EW (450 g/hm^2), 10% difenoconazole WG (900 g/hm^2, CK) or 24% thifluzamide SC (360 ml/hm^2) were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of 'modern agriculture, green agriculture, organic agriculture and ecological agriculture.
文摘To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan + Gibberellin-heteroauxin-brassinolide (GHB) and Ningnanmycin + GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml + GHB WP 600 g and 8% Ningnanmycin AS 900 ml + GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride (CK) + GHB. The obtained results indicated that Lentinan + GHB and Ningnanmycin + GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production.
文摘Aim To investigate the effects of milrinone (a selective phosphodiesteraseIII inhibitor PDE_3 ) on insulin secretion, blood glucose, plasma free fatty acids (FFA) anddose-response relationship, and assess possible effects of milrinone on glucose metabolism andinsulin sensitivity in conscious rats. Methods The catheterized nonstressed rats were administeredvarious doses of milrinone (1, 5, 25μmoL·kg^(-1)) and were compared with controls. Ahyperinsulinaemic-eugly-caemic clamp was established in counscious rats, andmilrinone(25μmoL·kg^(-1)) and 25% dimethyl sulfoxide (DMSO, as a control) were given at 120 minduring hyperinsulinaemic-euglycaemic clamping. Glucose turnover was determind with by gaschromatograph mass spectrometer (GC-MS). Results After dosing, plasma FFA levels in 3 milrinonegroups significantly increased, compared with the controls and before dosing. The percentages ofelevation of FFA by the different milrinone doses were very similar, 50%, and 52% , 55% for 1, 5,and 25 μmoL·kg^(-1), repectively, at 2 min after dosing. Plasma insulin levels were significantlyelevated in the 5 and 25 μmoL·kg^(-1) groups, and the effect of milrione on glucose concentrationwas detectable only in 25μmoL·kg^(-1) group. During hyperinsulinaemic clamping, there weresignificant increase, in plasma FFA (from 173 +- 15 to 634 +- 87μmoL·kg^(-1)) and hepatic glucoseproduction (HGP), and a significant decrease in glucose infusion rates (GIR) to about 21% and aslight increase in plasma insulin after milrinone treatment. Conclusion Milrinone impaires theability of insulin to suppress lipolysis and HGP, and insulin-mediated glucose utilization inperipheral tissue. Therefore, milrinone administration may induce an acute insulin resistance invivo.
文摘A general situation of the sugar beet industry and business in the European Union(EU) has been presented. It included the quotas of sugar production there, its prices and changes on the market. The latest sugar production data in the world was also given. The main companies carrying out activities on the Polish sugar and sugar beet seeds were also presented.New circumstances after liberating the sugar market in the EU in October, 2017, were also presented. Nowadays, Poland has been a great potential country for further technological progress in agricultural holdings and growing performance in sugar processing and production industry. As food export from Poland increases, it will be followed by increasing use of sugar. Examples are given to improve sugar beet productivity.
文摘The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water (WtF) from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence (CL) assay and hydroxyl free radical-initiated chemiluminescence (CL) assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.
基金supported by the National Natural Science Foundation of China(6137103921305067+5 种基金21105048)China Postdoctoral Science Foundation Funded Project(2012T50475)Science Foundation of Jiangsu Province(BK20130754)Ph D Fund of the Ministry of Education for Young Teachers(0133219120019)King Saud University(Distinguished Scientist Fellowship Program)Graduate Education Innovation Program of Nanjing University of Science and Technology and Chinese Academy of Sciences
文摘Organophosphorous pesticide(OP) contamination has serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA(ss DNA)-wrapped gold nanoparticles(Au NPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer(OBA) as the recognition element, aptamer-wrapped Au NPs(Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from Au NPs and the aggregation of Au NPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of Au NPs, the established assay showed good linearity between 0.1 and 10 μmol/L, with a low detection limit of 0.1 μmol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.