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白酒制曲环境和成品曲中产酸微生物分析 被引量:15
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作者 王春晓 袁国亿 +2 位作者 苏伟 王啸 邱树毅 《食品科学》 EI CAS CSCD 北大核心 2020年第18期120-126,共7页
为更好地控制小曲白酒风味,通过检测小曲白酒成品曲、成品曲糖化样品和制曲环境中的微生物,分析产生主要酸类物质的主导微生物,采用高效液相色谱法检测成品曲糖化样品中主要的酸类物质含量,采用高通量测序方法检测成品曲中细菌的相对丰... 为更好地控制小曲白酒风味,通过检测小曲白酒成品曲、成品曲糖化样品和制曲环境中的微生物,分析产生主要酸类物质的主导微生物,采用高效液相色谱法检测成品曲糖化样品中主要的酸类物质含量,采用高通量测序方法检测成品曲中细菌的相对丰度和多样性,采用传统分离筛选和序列分析方法鉴定成品曲糖化样品和制曲环境中的主导产乙酸微生物,并通过实验室糖化样品制备验证该微生物的产酸能力。结果表明:小曲白酒成品曲糖化样品中主要的酸类物质为乳酸和乙酸,乳酸的形成与成品曲中的乳杆菌属(Lactobacillus)、魏斯氏菌属(Weissella)和片球菌属(Pediococcus)多个主导菌种有关,乙酸的形成与制曲环境和成品曲糖化样品中的扣囊复膜酵母(Saccharomycopsis fibuligera)有关,醋酸菌几乎未检测到,因此推断与乙酸的形成相关性较小。本研究报道了扣囊复膜酵母的产乙酸能力,为其在白酒酸类物质形成中的研究和利用提供了新的思路。 展开更多
关键词 白酒 糖化样品 高通量测序 扣囊复膜酵母 酸度检测
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A New Approach for the Measurement of Digestible Carbohydrates in Different Food Samples with HPLC-RI
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作者 S. Devindra T. Longvah 《Journal of Agricultural Science and Technology(B)》 2011年第8期1216-1223,共8页
This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestibl... This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestible carbohydrates such as sugars and starches in different branded rice, legumes and vegetable samples which were procured from local market of twin cities of Hyderabad and Secunderabad in Andhra Pradesh State, India. In the present study, we estimated carbohydrates in vitro, mimicking the in vivo condition by incorporating enzymatic digestion. Among the rice varieties the analyzed total soluble sugars were in the range of 5.69% to 9.62%, vegetables 0.0% to 3.72% and legumes 0.04% to 0.98%. Soluble starches in rice samples ranged from 12.51% to 17.64%, in vegetables from 1.73% to 7.28% and in legumes from 23.76% to 38.71%. Insoluble starches in rice samples were observed to be bracketed in between 52.53% to 60.43%, where as in vegetables from 0.58% to 8.83% and in legumes from 16.13% to 29.22%. Concluding our observation, the total amount of starches and total sugars in rice fell in between 74.32% to 80.75%, in vegetables from 1.74% to 16.11%, and in legumes from 39.93% to 68.91%. 展开更多
关键词 STARCHES a-amylase PROTEASE AMYLOGLUCOSIDASE HPLC-RI method digestible carbohydrates.
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