期刊文献+
共找到12篇文章
< 1 >
每页显示 20 50 100
用活性多酶糖化菌发酵鸡粪作饲料
1
作者 张彬 《中国家禽》 北大核心 2002年第6期25-25,共1页
关键词 活性糖化 发酵 鸡粪 饲料
下载PDF
蜜蜂消化道酶谱带分布及其糖转化酶活性 被引量:3
2
作者 缪晓青 李江红 +3 位作者 吴珍红 张其康 孙亮先 陈刚 《福建农业大学学报》 CSCD 2000年第1期95-98,共4页
采用聚丙烯酰胺凝胶电泳分离意蜂和中蜂消化道各部分 (前肠、中肠、后肠 )肠液 .结果表明意蜂和中蜂消化酶的种类有明显差别 .它们的前肠均中有 1条酶带 (R1带 ) ,中肠均有 5条酶带 (意蜂为 R1、R3 、R4 、R5、R9;中蜂为 R1、R3 、R4 ... 采用聚丙烯酰胺凝胶电泳分离意蜂和中蜂消化道各部分 (前肠、中肠、后肠 )肠液 .结果表明意蜂和中蜂消化酶的种类有明显差别 .它们的前肠均中有 1条酶带 (R1带 ) ,中肠均有 5条酶带 (意蜂为 R1、R3 、R4 、R5、R9;中蜂为 R1、R3 、R4 、R6、R10 ) ,意蜂后肠有 5条酶带 (R1、R2 、R4 、R7、R8) ,中蜂则没有 .意蜂和中蜂的前肠、中肠、后肠的糖化酶活性均依次降低 .前肠和中肠的糖化酶活性意蜂较中蜂低 。 展开更多
关键词 消化道 糖化酶活性 意蜂 中蜂
下载PDF
SP 对发酵的促进作用研究 被引量:1
3
作者 王志民 谌伦高 +1 位作者 冉波 陈俊 《四川工业学院学报》 1997年第4期78-81,共4页
SP是由农作物中提取的天然成分加工而成的一种新型食品添加剂。低浓度的SP对提高酵母细胞的增殖、SCP产率和糖化酶活力都有明显地促进作用。
关键词 发酵促进剂 酵母细胞增殖 SCP 糖化酶活性
下载PDF
Study on Anti-oxidation and Inhibitory Effect on Nonenzymatic Glycation Reaction of Fermentation Extract from Biotransformation of Ginkgo biloba L. (EGB) by Hericium erinaceus 被引量:3
4
作者 黄达明 常为众 +3 位作者 张志才 崔凤杰 肖香 钱静亚 《Agricultural Science & Technology》 CAS 2008年第2期10-13,共4页
[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ab... [ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus. [ Result ] After adsorbed by AB-8 macroporous resin, the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin. [ Conclusion] AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus. 展开更多
关键词 BIOTRANSFORMATION ANTI-OXIDATION Nonenzymatic glycation reaction
下载PDF
Extracellular Enzyme Activity of Sclerotia-forming and Non-Sclerotia-forming Morchella Strains 被引量:3
5
作者 陈立佼 王芳 +2 位作者 赵永昌 黄兴奇 柴红梅 《Agricultural Science & Technology》 CAS 2013年第10期1392-1396,1408,共6页
The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica... The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica exhibited higher aver- age activities of protease, catalase and xylanase but lower laccase activity than those of sclerotia-forming strains. However, the differences did not reach significance level. The soluble amylases activity of M. crasspies was significantly higher than that of M. conica, while the mean activity of other enzymes had differences between them, but the differences did not reach significance level. 展开更多
关键词 Morchella conica Morchella crasspies SCLEROTIA Extracellular enzyme
下载PDF
Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury 被引量:5
6
作者 Xiao-ShunHe YiMa +4 位作者 Lin-WeiWu Jin-LangWu Rui-DeHu Gui-HuaChen Jie-FuHuang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第17期2662-2665,共4页
AIM:To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm ischemia time (WIT) and to predict the tolerant time limitation of the liver graft to ... AIM:To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm ischemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury. METHODS: The rats were randomized into five groups, WIT was 0,15,30,45,60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation. RESULTS: The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min. CONCLUSION: Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts. 展开更多
关键词 Liver transplantation Warm ischemia injury Histochemical stain
下载PDF
Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats 被引量:23
7
作者 Wen-Peng Yuan Bing Liu +4 位作者 Chang-Heng Liu Xiao-Jun Wang Mian-Song Zhang Xiu-Mei Meng Xue-Kui Xia 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第11期1339-1345,共7页
AIM:To investigate the antioxidant activity of chitooligosaccharides(COSs)on pancreatic islet cells in diabetic rats induced by streptozotocin. METHODS:The antioxidant effect of COSs on pancreatic islet cells was dete... AIM:To investigate the antioxidant activity of chitooligosaccharides(COSs)on pancreatic islet cells in diabetic rats induced by streptozotocin. METHODS:The antioxidant effect of COSs on pancreatic islet cells was detected under optical microscopy and with colorimetric assay and gel electrophoresis.The activities of glutathione peroxidase and superoxide dismutase,total antioxidant capacity,and content of malondialdehyde in serum and tissue slices of pancreas were examined after 60 d to determine the effect of COSs in streptozotocin-induced diabetes in rats. RESULTS:COSs can prohibit the apoptosis of pancreatic islet cells.All concentrations of COSs can improve the capability of total antioxidant capacity and activity of superoxide dismutase and decrease the content of malondialdehyde drastically.Morphological investigation in the pancreas showed that COSs have resulted in the reduction of islets,loss of pancreatic cells,and nuclear pyknosis of pancreatic cells. CONCLUSION:COSs possess various biological activities and can be used in the treatment of diabetes mellitus. 展开更多
关键词 Chito-oligosaccharides Diabetes mellitus APOPTOSIS Antioxidant ability Pancreastic islet cells STREPTOZOTOCIN
下载PDF
Effects of Dietary β-Glucan and Glycyrrhizin on Non-Specific Immunity and Disease Resistance of the Sea Cucumber (Apostichopus japonicus Selenka) Challenged with Vibrio splendidus 被引量:13
8
作者 CHANG Jie ZHANG Wenbing +2 位作者 MAI Kangsen MA Hongming XU Wei 《Journal of Ocean University of China》 SCIE CAS 2010年第4期389-394,共6页
Sea cucumbers, Apostichopus japonicus Selenka, were fed diets containing non-immunostimulant (basal diet), 0.2% β-glucan and 0.02% glycyrrhizin in a recirculatory water system for 45 days, and subsequently challenged... Sea cucumbers, Apostichopus japonicus Selenka, were fed diets containing non-immunostimulant (basal diet), 0.2% β-glucan and 0.02% glycyrrhizin in a recirculatory water system for 45 days, and subsequently challenged with Vibrio splendidus by injection at 1.0×108 cfu/sea cucumber for 15 days.Phagocytic capacity (PC), intracellular superoxide anion production (ISAP), ly-sozyme (LSZ) activity and superoxide dismutase (SOD) activity in the coelomic fluid were analyzed on the 0th, 5th, 10th and 15th days after injection.Results showed that after the 45-day feeding period, PC, ISAP, LSZ activity and SOD activity in sea cucumbers fed with dietary β-glucan or glycyrrhizin were significantly higher than in those fed with the basal diet.On the 5th day after infection, all the immune parameters examined in the sea cucumbers injected with V.splendidus decreased in value significantly.On the 15th day, PC, ISAP and LSZ activity returned to levels similar to those on the 0th day.For the sea cucumbers injected with saline, there were no significant differences in all the immune parameters examined and in the cumulative morbidity during the 15-day challenging trial.After injecting with V.splendidus, the cumulative morbidity of sea cucumbers fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin when challenged with V.splendidus challenged sea cucumber fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin.There was no significant difference in cumulative morbidity between the dietary β-glucan and glycyrrhizin treatments over time. 展开更多
关键词 Β-GLUCAN GLYCYRRHIZIN Apostichopus japonicus Selenka Immunity Vibrio splendidus
下载PDF
Effect of Shenqi Compound Formula on PPARγ in White Adipose Tissue of Rats with Macrovascular Lesion in Early Stage of Diabetes
9
作者 张红敏 谢春光 +2 位作者 陈世伟 谢毅强 王友京(译) 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2008年第2期134-138,共5页
Objective:To investigate the effect of Shenqi Compound Formula (SCF) on peroxisome proliferators-activated receptor γ (PPARγ) in white adipose tissue of rats with macrovascular lesion in early stage of diabetes. Met... Objective:To investigate the effect of Shenqi Compound Formula (SCF) on peroxisome proliferators-activated receptor γ (PPARγ) in white adipose tissue of rats with macrovascular lesion in early stage of diabetes. Methods: Corresponding treatment was given to rats in model group, Ramipril group, normal control group, low dosage SCF group and high dosage SCF group respectively for 32 days. The expressions of PPARγ and adiponectin Messenger RNA (mRNA) were detected by real-time reverse transcriptase poly-merase chain reaction. Results: The expressions of PPARγ and adiponectin mRNA increased significantly in both low and high dosage SCF groups as compared with the model group, and a positive linear correlation was found between the expressions of PPARγ and adiponectin mRNA. Conclusions: SCF can prevent macrovascular lesion in early stage of diabetes, which is possibly related with up-regulating expressions of PPARγ and activating PPARγ. 展开更多
关键词 Shenqi Compound Formula TypeⅡ Diabetes Macrovascular lesion Peroxisome proliferators-activated receptor γ (PPARγ)
下载PDF
Membrane Microfiltration Fermentation of Glucose Oxidase with Cell Recycling
10
作者 储炬 江洁 +1 位作者 李友荣 俞俊棠 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1999年第1期30-37,共8页
Membrane microfiltration fermentation (MMF) with cell recycling was successfully applied to the production of glucose oxidase (GOD). A plate microfiltration module was found suitable for such purpose. By feeding whole... Membrane microfiltration fermentation (MMF) with cell recycling was successfully applied to the production of glucose oxidase (GOD). A plate microfiltration module was found suitable for such purpose. By feeding whole medium in MMF, the productivity of GOD was much higher than that by feeding glucose alone. With increasing dilution rate the enzyme productivity increased and average enzyme activity decreased. The enzyme productivity of MMF under D = 0.12h-1 and 0.20h-1 were 3871 and 3945U·h-1 respectively, which was about 3 times as that of batch fermentation (BF) and the average enzyme activity was still as high as STU·mL-1 under D = 0.12h-1. The relative efficiency of MMF applied to low yield strain was higher than that applied to high yield strain. 展开更多
关键词 glucose oxidase membrane microfiltration fermentation cell recycling acetylcellulose membrane
下载PDF
Soil Enzyme Activities on Eroded Slopes in the Sichuan Basin, China 被引量:7
11
作者 NIE Xiaojun ZHANG Jianhui GAO Han 《Pedosphere》 SCIE CAS CSCD 2015年第4期489-500,共12页
Determining how soil erosion affects enzyme activity may enhance our understanding of soil degradation on eroded agricultural landscapes. This study assessed the changes in enzyme activity with slope position and eros... Determining how soil erosion affects enzyme activity may enhance our understanding of soil degradation on eroded agricultural landscapes. This study assessed the changes in enzyme activity with slope position and erosion type by selecting water and tillage erosion-dominated slopes and performing analyses using the 1376s technique. The 137Cs data revealed that soil loss occurred in the upper section of the two eroded slope types, while soil accumulation occurred in the lower section. The invertase activity increased downslope and exhibited a pattern similar to the 137Cs data. The spatial patterns of urease and alkaline phosphatase activities were similar to the 137Cs inventories on the water and tillage erosion-dominated slopes, respectively. On both the eroded slope types, the invertase activity and soil organic carbon content were correlated, but no correlation was observed between the alkaline phosphatase activity and total phosphorus content. Nevertheless, the urease activity was correlated with the total nitrogen content only on the water erosion-dominated slopes. The enzyme activity-to-microbial biomass carbon ratios indicated high activities of invertase and urease but low activity of phosphatase on the water erosion-dominated slopes compared with the tillage erosion-dominated slopes. Both the invertase activity and the invertase activity-to-microbial biomass carbon ratio varied with the slope position. Changes in the urease activity-to-microbial biomass carbon ratio were significantly affected by the erosion type. These suggested that the dynamics of the invertase activity were linked to soil redistribution on the two eroded slope types, whereas the dynamics of the urease and alkaline phosphatase activities were associated with soil redistribution only on the water or tillage erosion-dominated slopes, respectively. The erosion type had an obvious effect on the activities of invertase, urease and alkaline phosphatase. Soil redistribution might influence the involvement of urease in the N cycle and alkaline phosphatase in the P cycle. Thus, enzyme activity-to-microbial biomass ratios may be used to better evaluate microbiological activity in eroded soils. 展开更多
关键词 137Cs technique microbial biomass C N cycle P cycle soil organic C soil redistribution tillage erosion water erosion
原文传递
Growth, Cadmium Accumulation and Physiology of Marigold (Tagetes erecta L.) as Affected by Arbuscular Mycorrhizal Fungi 被引量:12
12
作者 LIU Ling-Zhi GONG Zong-Qiang +1 位作者 ZHANG Yu-Long LI Pei-Jun 《Pedosphere》 SCIE CAS CSCD 2011年第3期319-327,共9页
A pot experiment was carried out to study the effects of three arbuscular mycorrhizal fungi (AMF), including Glomus intraradices, Glomus constrictum and Glomus mosseae, on the growth, root colonization and Cd accumu... A pot experiment was carried out to study the effects of three arbuscular mycorrhizal fungi (AMF), including Glomus intraradices, Glomus constrictum and Glomus mosseae, on the growth, root colonization and Cd accumulation of marigold (Tagetes erecta L.) at Cd addition levels of 0, 5 and 50 mg kg-1 in soil. The physiological characteristics, such as chlorophyll content, soluble sugar content, soluble protein content and antioxidant enzyme activity, of Tagetes erecta L. were also investigated. The symbiotic relationship between the marigold plant and arbuscular mycorrhizal fungi was well established under Cd stress. The symbiotic relationship was reflected by the better physiobiochemical parameters of the marigold plants inoculated with the three AMF isolates where the colonization rates in the roots were between 34.3% and 88.8%. Compared with the non-inoculated marigold plants, the shoot and root biomass of the inoculated marigold plants increased by 15.2%- 47.5% and 47.8%-130.1%, respectively, and the Cd concentration and accumulation decreased. The chlorophyll and soluble sugar contents in the mycorrhizal marigold plants increased with Cd addition, indicating that AMF inoculation helped the marigold plants to grow by resisting Cd stress. The antioxidant enzymes reacted differently with the three AMF under Cd stress. For plants inoculated with G. constrictum and G. mosseae, the activities of superoxide dismutase (SOD) and catalase (CAT) increased with increasing Cd addition, but peroxidase (POD) activity decreased with increasing Cd addition. For plants inoculated with G. intraradices, three of the antioxidant enzyme activities were significantly decreased at high levels of Cd addition. Overall, the activities of the three antioxidant enzymes in the plants inoculated with AMF were higher than those of the plants without AMF inoculation under Cd stress. Our results support the view that antioxidant enzymes have a great influence on the biomass of plants, and AMF can improve the capability of reactive oxygen species (ROS) scavenging and reduce Cd concentration in plants to alleviate Tagetes erecta L. from Cd stress. 展开更多
关键词 antioxidant enzyme Cd stress metal accumulation PHYTOREMEDIATION root colonization
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部