Curcuma wenyujin has been widely used as a traditional medicine in China. In this paper a strategy for the quantitative determination of the polysaccharide by a phenol-sulfuric acid method was described. Involved in t...Curcuma wenyujin has been widely used as a traditional medicine in China. In this paper a strategy for the quantitative determination of the polysaccharide by a phenol-sulfuric acid method was described. Involved in three factors, 5% phenol volume, H2SO4 volume, and temperature of water bath, we adopted the L9（3）3 orthogonal array design to gain the optimal colorimetric method. 3.0 ml of polysaccharide solution, 1.0 ml, 5% phenol and 7.0 ml H2SO4 were mixed with constant stirring in a glass vessel, and then kept in a water bath at 40 ℃. After cooling to room temperature for 20 min, the absorbance values were recorded by the UV-2501 PC spectrometer at the wavelength range of 485 nm. The polysaccharide content in Curcuma wenyujin were 3.21%, 3.23%, 3.20%, 3.18~/0, 3.22% and 2.38%, respectively. All results showed that this method was adequate, valid and applicable, may be applied to the determination of other bacterial polysaccharide as well.展开更多
Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile ph...Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile phase system consisted of ammonium acetate buffer： Methanol （60：40 v/v） at a flow rate of 1.0 mL.min^-1, and UV detector was set at 235 nm. The calibration curve of saccharin was linear in the concentration range between 1 to 100 mg·L^-1）, while the detection limit of saccharin was found to be 0.08 mg.L^-1. The mean value of recovery was 98.24% with standard deviation of0.32% （n=12）. The proposed method was successfully applied to determine the amount of saccharin in 12 preserved fruits, commercially available in Chiang Mai local markets. The results showed that the saccharin contents were found in the range of 6.22-78.60 mg.kg^-1.展开更多
In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （...In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （XEZKTJ） with standardized reference extract （SRE）. The five analytes （liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid） were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract （SRE method） and individual chemical reference standards （CRS method）. Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study.展开更多
文摘Curcuma wenyujin has been widely used as a traditional medicine in China. In this paper a strategy for the quantitative determination of the polysaccharide by a phenol-sulfuric acid method was described. Involved in three factors, 5% phenol volume, H2SO4 volume, and temperature of water bath, we adopted the L9（3）3 orthogonal array design to gain the optimal colorimetric method. 3.0 ml of polysaccharide solution, 1.0 ml, 5% phenol and 7.0 ml H2SO4 were mixed with constant stirring in a glass vessel, and then kept in a water bath at 40 ℃. After cooling to room temperature for 20 min, the absorbance values were recorded by the UV-2501 PC spectrometer at the wavelength range of 485 nm. The polysaccharide content in Curcuma wenyujin were 3.21%, 3.23%, 3.20%, 3.18~/0, 3.22% and 2.38%, respectively. All results showed that this method was adequate, valid and applicable, may be applied to the determination of other bacterial polysaccharide as well.
文摘Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile phase system consisted of ammonium acetate buffer： Methanol （60：40 v/v） at a flow rate of 1.0 mL.min^-1, and UV detector was set at 235 nm. The calibration curve of saccharin was linear in the concentration range between 1 to 100 mg·L^-1）, while the detection limit of saccharin was found to be 0.08 mg.L^-1. The mean value of recovery was 98.24% with standard deviation of0.32% （n=12）. The proposed method was successfully applied to determine the amount of saccharin in 12 preserved fruits, commercially available in Chiang Mai local markets. The results showed that the saccharin contents were found in the range of 6.22-78.60 mg.kg^-1.
基金Drug Standards Improvement Project of Chinese Pharmacopoeia Commission
文摘In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （XEZKTJ） with standardized reference extract （SRE）. The five analytes （liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid） were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract （SRE method） and individual chemical reference standards （CRS method）. Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study.
