We studied 14 moderately overweight Typo 2 diabetic patients with essential hypertension in stable metabolic control after a run-in period , and again after 3 months of antihypertensive treatment with the angiotensin-...We studied 14 moderately overweight Typo 2 diabetic patients with essential hypertension in stable metabolic control after a run-in period , and again after 3 months of antihypertensive treatment with the angiotensin-converting enzyme (ACF) inhibitor captopril. Glucose tolerance was tested with a 75g oral glucose load (OGTT) and insulin sensitivity was measured by the insulin suppression test (IST) while dietary and drug treatment of the hyperglycemia was maintained constant. In the whole group. mean blood pressure (MBP) fell progressively over 3months from a baseline value of 123± 3 mmHg (1 mmHg= 0. 133 kpa) to a final value of 115± 2 mmHg(P<0. 005). After treatment, fasting plasma glucose, insulin, free fatty acid (FFA). potassium, and glycosylated hemoglobin concentrations were unchanged from baseline. There were no significant differences in glucose tolerance and insulin sensitivity between pre- and post-trearment values. Neither endogenous (oral glucose) nor exogenous (IST) insulin caused any change in plasma potassium concentration. This resistance to the hypokalemic action of insulin was not affected by captopril.展开更多
Hemolytic anemia after implantation of an artificial mitral valve is a rare but recognized complication of surgery. The presence of paravalvular leak after surgery and the necessity of using oral anticoagulants causes...Hemolytic anemia after implantation of an artificial mitral valve is a rare but recognized complication of surgery. The presence of paravalvular leak after surgery and the necessity of using oral anticoagulants causes that anemia emerges as a severe and potentially fatal issue.展开更多
Objective： To observe the effect of electroacupuncture（EA） on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis（DGP）.Methods： Fifty Sprague-Dawley（SD） rats were randomly divi...Objective： To observe the effect of electroacupuncture（EA） on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis（DGP）.Methods： Fifty Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E,10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin（STZ）, combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/（kg·bw）] by gavage. Rat＇s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420 F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay（ELISA）; the expression of ghrelin mRNA was detected by real-time polymerase chain reaction（RT-PCR）.Results： Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment（all P〈0.01）; after treatment, the gastric emptying rate of group B was significantly decreased（P〈0.01）,the migration rates of small intestine in group B, C, D and E were significantly lower（all P〈0.01）, and the protein content of ghrelin in group C was significantly decreased（P〈0.01）; the expressions of ghrelin mRNA were significantly increased in group B, C, D and E（all P〈0.01）, the mean amplitudes of electrogastrogram in group B and D were significantly decreased（both P〈0.01）. After treatment, compared with group B, the blood glucose of group C was significantly decreased（P〈0.05）, the gastric emptying rate and small intestine migration rate were significantly increased in group C and E（P〈0.05, P〈0.01）, the small intestinal migration rate was significantly increased in group D（P〈0.05）, the expression of ghrelin in protein and mRNA in group C was significantly lower（P〈0.01）, the expression of ghrelin mRNA in group E was significantly lower（P〈0.05）, and the mean amplitude of electrogastrogram in group C was significantly increased（P〈0.05）. After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased（P〈0.01）. After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased（P〈0.01）.Conclusion： EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6） could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.展开更多
OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to invest...OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to investigate the antiglycation and antioxidation potential of two medicinal plants:Juglans regia and Calendula officinalis.METHODS:In-vitro investigation was carried out to discover the antiglycation and antioxidation potential of J.regia and C.officinalis.Using an Ultraviolet Double-beam Spectrophotometer,we evaluated the antiglycation property of the crude methanolic extracts of J.regia and C.officinalis by assessing their ability to inhibit the Maillard reaction.Employing the same instrument we also measured the antioxidation potential of these plant extracts using the nitric oxide(NO) free radical-scavenging assay.RESULTS:J.regia had greater antiglycation ability,with a minimum inhibitory concentration(MIC 50) of 28 μg/mL as compared with that of C.officinalis(270 μg/mL).C.officinalis had greater antioxidation potential(26.10,22.07 and 16.06% at 0.5 mg,0.25 mg and 0.125 mg,respectively,as compared with 18.15,16.50 and 16.06% of J.regia,respectively).CONCLUSION:J.regia and C.officinalis inhibited the Maillard reaction and prevented oxidation in-vitro.Hence,the extracts of these plants could have therapeutic uses in curbing chronic diabetic complications and slowing down aging.展开更多
文摘We studied 14 moderately overweight Typo 2 diabetic patients with essential hypertension in stable metabolic control after a run-in period , and again after 3 months of antihypertensive treatment with the angiotensin-converting enzyme (ACF) inhibitor captopril. Glucose tolerance was tested with a 75g oral glucose load (OGTT) and insulin sensitivity was measured by the insulin suppression test (IST) while dietary and drug treatment of the hyperglycemia was maintained constant. In the whole group. mean blood pressure (MBP) fell progressively over 3months from a baseline value of 123± 3 mmHg (1 mmHg= 0. 133 kpa) to a final value of 115± 2 mmHg(P<0. 005). After treatment, fasting plasma glucose, insulin, free fatty acid (FFA). potassium, and glycosylated hemoglobin concentrations were unchanged from baseline. There were no significant differences in glucose tolerance and insulin sensitivity between pre- and post-trearment values. Neither endogenous (oral glucose) nor exogenous (IST) insulin caused any change in plasma potassium concentration. This resistance to the hypokalemic action of insulin was not affected by captopril.
文摘Hemolytic anemia after implantation of an artificial mitral valve is a rare but recognized complication of surgery. The presence of paravalvular leak after surgery and the necessity of using oral anticoagulants causes that anemia emerges as a severe and potentially fatal issue.
基金supported by National Natural Science Foundation of China,No.81403487National Basic Research Program of China,No.2014CB543102Youth Fund of Hunan Province Education Office,No.14B128~~
文摘Objective： To observe the effect of electroacupuncture（EA） on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis（DGP）.Methods： Fifty Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E,10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin（STZ）, combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/（kg·bw）] by gavage. Rat＇s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420 F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay（ELISA）; the expression of ghrelin mRNA was detected by real-time polymerase chain reaction（RT-PCR）.Results： Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment（all P〈0.01）; after treatment, the gastric emptying rate of group B was significantly decreased（P〈0.01）,the migration rates of small intestine in group B, C, D and E were significantly lower（all P〈0.01）, and the protein content of ghrelin in group C was significantly decreased（P〈0.01）; the expressions of ghrelin mRNA were significantly increased in group B, C, D and E（all P〈0.01）, the mean amplitudes of electrogastrogram in group B and D were significantly decreased（both P〈0.01）. After treatment, compared with group B, the blood glucose of group C was significantly decreased（P〈0.05）, the gastric emptying rate and small intestine migration rate were significantly increased in group C and E（P〈0.05, P〈0.01）, the small intestinal migration rate was significantly increased in group D（P〈0.05）, the expression of ghrelin in protein and mRNA in group C was significantly lower（P〈0.01）, the expression of ghrelin mRNA in group E was significantly lower（P〈0.05）, and the mean amplitude of electrogastrogram in group C was significantly increased（P〈0.05）. After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased（P〈0.01）. After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased（P〈0.01）.Conclusion： EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6） could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.
文摘OBJECTIVE:Accumulation of advanced glycation end products(AGEs) in the body due to the non-enzymatic glycation of proteins and oxidation is associated with aging and diabetes mellitus.In this study we wanted to investigate the antiglycation and antioxidation potential of two medicinal plants:Juglans regia and Calendula officinalis.METHODS:In-vitro investigation was carried out to discover the antiglycation and antioxidation potential of J.regia and C.officinalis.Using an Ultraviolet Double-beam Spectrophotometer,we evaluated the antiglycation property of the crude methanolic extracts of J.regia and C.officinalis by assessing their ability to inhibit the Maillard reaction.Employing the same instrument we also measured the antioxidation potential of these plant extracts using the nitric oxide(NO) free radical-scavenging assay.RESULTS:J.regia had greater antiglycation ability,with a minimum inhibitory concentration(MIC 50) of 28 μg/mL as compared with that of C.officinalis(270 μg/mL).C.officinalis had greater antioxidation potential(26.10,22.07 and 16.06% at 0.5 mg,0.25 mg and 0.125 mg,respectively,as compared with 18.15,16.50 and 16.06% of J.regia,respectively).CONCLUSION:J.regia and C.officinalis inhibited the Maillard reaction and prevented oxidation in-vitro.Hence,the extracts of these plants could have therapeutic uses in curbing chronic diabetic complications and slowing down aging.
