Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch ...Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch medium, and the highest rate was obtained in the initial growth phase, after incubation for 24 h at pH 5.5. Maximum amylase and glucoamylase activities （483.62 U mg^-1 protein and 290.85 U mg^-1 protein） were obtained at pH 5.5. The isolated enzymes exhibited thermostable properties as indicated by retention of 100% of residual activity at 55 ℃ for 45 min with total inhibition at 100 ℃. Extracellular enzyme from S diastaticus 2047 was partially purified by fractionated precipitation with ammonium sulphate. After 40% saturation produced 2,197.00 and 1,192.83 U/mg protein, and yield was 40% with purification 4.54 and 4.1 fold, respectively. This study presents feasibility on ethanol production from cassava pulps pretreated with diluted sulfuric acid by simultaneous saccharification and fermentation （SSF） with S. diastaticus 2047. The results indicated that the culture was able to produce ethanol with high yield without amylolytic enzyme adding by using cassava pulps pretreated with distilled water at 135 ℃ under pressure of 15 lb/inch^2 to produce ethanol yield as high as the cassava pulps pretreated with diluted sulfuric acid under the same condition. This suggests that S diastaticus with enzyme produced has potential for industrial applications.展开更多
文摘Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch medium, and the highest rate was obtained in the initial growth phase, after incubation for 24 h at pH 5.5. Maximum amylase and glucoamylase activities （483.62 U mg^-1 protein and 290.85 U mg^-1 protein） were obtained at pH 5.5. The isolated enzymes exhibited thermostable properties as indicated by retention of 100% of residual activity at 55 ℃ for 45 min with total inhibition at 100 ℃. Extracellular enzyme from S diastaticus 2047 was partially purified by fractionated precipitation with ammonium sulphate. After 40% saturation produced 2,197.00 and 1,192.83 U/mg protein, and yield was 40% with purification 4.54 and 4.1 fold, respectively. This study presents feasibility on ethanol production from cassava pulps pretreated with diluted sulfuric acid by simultaneous saccharification and fermentation （SSF） with S. diastaticus 2047. The results indicated that the culture was able to produce ethanol with high yield without amylolytic enzyme adding by using cassava pulps pretreated with distilled water at 135 ℃ under pressure of 15 lb/inch^2 to produce ethanol yield as high as the cassava pulps pretreated with diluted sulfuric acid under the same condition. This suggests that S diastaticus with enzyme produced has potential for industrial applications.
