Hereditary fructose intolerance(HFI) is an underrecognized,preventable life-threatening condition.It is an autosomal recessive disorder with subnormal activity of aldolase B in the liver,kidney and small bowel.Symptom...Hereditary fructose intolerance(HFI) is an underrecognized,preventable life-threatening condition.It is an autosomal recessive disorder with subnormal activity of aldolase B in the liver,kidney and small bowel.Symptoms are present only after the ingestion of fructose,which leads to brisk hypoglycemia,and an individual with continued ingestion will exhibit vomiting,abdominal pain,failure to thrive,and renal and liver failure.A diagnosis of HFI was made in a 50-year-old woman on the basis of medical history,response to fructose intolerance test,demonstration of aldolase B activity reduction in duodenal biopsy,and molecular analysis of leukocyte DNA by PCR showed homozygosity for two doses of mutant gene.HFI may remain undiagnosed until adult life and may lead to disastrous complications following inadvertent fructose or sorbitol infusion.Several lethal episodes of HFI following sorbitol and fructose infusion have been reported.The diagnosis can only be suspected by taking a careful dietary history,and this can present serious complications.展开更多
A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicini...A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chro-mosomes 6 and 14. The in situ results using digoxygenin labelling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cyto-genetic analysis of very small chromosomes (1.0-2.7μm)of apple.展开更多
Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile ph...Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile phase system consisted of ammonium acetate buffer： Methanol （60：40 v/v） at a flow rate of 1.0 mL.min^-1, and UV detector was set at 235 nm. The calibration curve of saccharin was linear in the concentration range between 1 to 100 mg·L^-1）, while the detection limit of saccharin was found to be 0.08 mg.L^-1. The mean value of recovery was 98.24% with standard deviation of0.32% （n=12）. The proposed method was successfully applied to determine the amount of saccharin in 12 preserved fruits, commercially available in Chiang Mai local markets. The results showed that the saccharin contents were found in the range of 6.22-78.60 mg.kg^-1.展开更多
文摘Hereditary fructose intolerance(HFI) is an underrecognized,preventable life-threatening condition.It is an autosomal recessive disorder with subnormal activity of aldolase B in the liver,kidney and small bowel.Symptoms are present only after the ingestion of fructose,which leads to brisk hypoglycemia,and an individual with continued ingestion will exhibit vomiting,abdominal pain,failure to thrive,and renal and liver failure.A diagnosis of HFI was made in a 50-year-old woman on the basis of medical history,response to fructose intolerance test,demonstration of aldolase B activity reduction in duodenal biopsy,and molecular analysis of leukocyte DNA by PCR showed homozygosity for two doses of mutant gene.HFI may remain undiagnosed until adult life and may lead to disastrous complications following inadvertent fructose or sorbitol infusion.Several lethal episodes of HFI following sorbitol and fructose infusion have been reported.The diagnosis can only be suspected by taking a careful dietary history,and this can present serious complications.
文摘A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chro-mosomes 6 and 14. The in situ results using digoxygenin labelling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cyto-genetic analysis of very small chromosomes (1.0-2.7μm)of apple.
文摘Quantitative analysis of saccharin in preserved fruits was performed by High Performance Liquid Chromatography （HPLC）. The separation was observed on a reversed phase ODS C）8 column （4.6 × 250 mm）. Mobile phase system consisted of ammonium acetate buffer： Methanol （60：40 v/v） at a flow rate of 1.0 mL.min^-1, and UV detector was set at 235 nm. The calibration curve of saccharin was linear in the concentration range between 1 to 100 mg·L^-1）, while the detection limit of saccharin was found to be 0.08 mg.L^-1. The mean value of recovery was 98.24% with standard deviation of0.32% （n=12）. The proposed method was successfully applied to determine the amount of saccharin in 12 preserved fruits, commercially available in Chiang Mai local markets. The results showed that the saccharin contents were found in the range of 6.22-78.60 mg.kg^-1.
