A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen ...A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.展开更多
[ Objective] The aim was to improve the saccharification of wheat bran cellulose. [ Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times an...[ Objective] The aim was to improve the saccharification of wheat bran cellulose. [ Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times and substrate concentration on the saccharification were investigated. [ Result] The influence of temperature on the acid treatment of the sacchadfication of wheat bran cellulose was significant. Influences of acid concentration on the hydrolysis were distinct. Influences of time and substrate concentration were insignificant. The optimal pretreatment conditions were 1.5% of sulfuric acid concentration and 0. 067 g/ml of substrate concentration at 100℃ in three hours. Under this condition, the sugar concentration was 38.137 mg/ml, and the hydrolysis rate reached 51.485%. [ Conclusion] The study improved the saccharification of wheat bran cellulose, which provided theoretical basis for the application of wheat bran industrial process.展开更多
文摘A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.
基金Supported by National Natural Fund Program(31071636)Anhui Science and Technology Bureau Program(1206C0805017/KJ2010A262)
文摘[ Objective] The aim was to improve the saccharification of wheat bran cellulose. [ Method] Taking the wheat bran as raw materials, and using orthogonal method, the effects of acid concentration, temperature, times and substrate concentration on the saccharification were investigated. [ Result] The influence of temperature on the acid treatment of the sacchadfication of wheat bran cellulose was significant. Influences of acid concentration on the hydrolysis were distinct. Influences of time and substrate concentration were insignificant. The optimal pretreatment conditions were 1.5% of sulfuric acid concentration and 0. 067 g/ml of substrate concentration at 100℃ in three hours. Under this condition, the sugar concentration was 38.137 mg/ml, and the hydrolysis rate reached 51.485%. [ Conclusion] The study improved the saccharification of wheat bran cellulose, which provided theoretical basis for the application of wheat bran industrial process.
