附子理中丸类制剂中4种糖成分的含量测定

目的 建立同时测定附子理中丸类制剂中蜂蜜的4种糖成分含量的方法。方法 采用高效液相色谱法,以Prevail Carbohyrate ES为色谱柱,乙腈-水为流动相,梯度洗脱,流速为1.0 ml/min,柱温30℃。结果 果糖、D-无水葡萄糖、蔗糖、麦芽糖分别在75.055 0~375.275 0、80.006 5~400.032 5、10.009 5~50.047 5、10.003 5~50.017 5μg范围内线性较好(r>0.999);加样回收率为94.7%~100.1%(RSD<2.0%,n=9)。结论 该方法科学准确,可用附子理中丸类制剂中辅料蜂蜜的质量控制。

关于煮糖起晶制种方法的探讨以及PKZF-I型专用种糊机的应用

论述了国内制糖企业采用的各种起晶制种方法的工作原理及特点 ,提出使用PKZF-I型煮糖专用种糊机制备糖种 。

从五种基本糖品的性能比较试论淀粉糖工业的发展

蔗糖、葡萄糖、果糖、麦芽糖和木糖五种基本糖品是人们日常食用最多的糖品，本文对这五种糖品的性能进行了综述和分析，对人们选择食用糖品和发展淀粉糖工业应有所裨益。

不同苹果产地长富2号多酚及糖类物质含量差异分析

分别以70%丙酮、水为苹果果实多酚和糖的提取溶剂,对全国范围内9个苹果特征产地的长富2号多酚、4种糖类物质含量差异进行了分析。结果显示:不同产地的长富2号多酚和糖组分含量差异显著,多酚含量最低的为云南昭通0.093 mg/g;较高的为新疆伊犁、陕西洛川、甘肃天水,含量分别为0.146、0.140、0.130 mg/g;4种糖组分总含量较高的为新疆伊犁383.3664 mg/g、甘肃天水342.7450 mg/g、山西太谷324.6702 mg/g。此外,对不同产区的多酚进行了聚类分析及主成分分析。

HPLC-CAD法同时测定乳制品中糖类

建立了高效液相色谱（HPLC）串联电喷雾检测器（CAD）同时测定乳制品中果糖、半乳糖、葡萄糖、蔗糖、乳糖和麦芽糖的方法。样品经沉淀剂沉淀蛋白，以Shodex Asahipak NH2P-504E为分离柱，乙腈一水为流动相，流速为1．0mL／min，采用电喷雾检测器检测。在该条件下，上述6种糖分离完全，线性良好，回收率在93．6％一104．3％，精密度为2．05％～5．59％，检出限分别为0．80，0．18，0．10，0．20，10．03，4．99Ng／mL。方法灵敏度高、准确可靠、安全环保，可用于乳制品中果糖、半乳糖、葡萄糖、蔗糖、乳糖和麦芽糖的质量浓度测定。

Selection of New Sugarcane Variety Guitang 42 with High Yield and High Sugar Content and Suitable for Mechanized Production

[Objective] This study was conducted to provide and promote high-yield,high-sugar, high-lodging resistance cane varieties and change the situation of singleness of sugarcane variety in Guangxi. [Method] The variety, Guitang 04-1001（Guitang 42）, a progeny of the cross ROC22 ×Guitang 92-66, was bred and evaluated in the aspects of morphological characteristics and production characteristics through the breeding program of a five-nursery-system protocol by both regional and production trials. [Results] Under many years of experiments, Guitang 42 was shown with high stalk, medium to large stalk diameter, easy stripping, good germination, medium tillering ability, and more population of millable stalks; and high resistance to mosaic and pokkah boeng, medium resistance to smut, high drought tolerance, and good ratooning ability. During the five-nursery-system procedure, the average of cane and sugar yield was 95.06-108.87 t/hm^2 and 15.11-16.85 t/hm^2, which were higher than CK by 11.3%-14.1% and 16.0%-18.9%, respectively. In the regional trial in 2011 and 2012, the achieved cane yield of 101.7 t/hm^2, sugar yield of 15.03 t/hm^2 and sucrose contents of 14.46% were higher than CK by 9.26%, 14.45% and0.66%, respectively. In the production trial in 2012, the average cane yield was88.62 t/hm^2, which was higher than ROC22 by 7.9%. Guitang 42 was approved by Guangxi Crop Variety Approval Committee in 2013 and elected to Guangxi Top 10 sugarcane commercial variety in 2014. [Conclusion] Guitang 42 has the characteristics of early maturity, high yield, high sugar content, good ratooning ability, and high resistance to lodging（i.e., good comprehensive traits）, and could be popularized in south sugarcane regions in China.

Varietal Characteristics and Nutrition Characteristics of Several New Varieties of High Biomass Sugarcanes for Sugar and Energy

[Objective] The aim was to study the varietal characteristics of six new varieties of high biomass sugarcanes for sugar and energy namely GT02-833,GT97-69,GT02-351,GT03-2287,B9 and GT02-770,as well as the nutrition characteristics of them under drip irrigation conditions. [Method] Industrial and agronomic characters,total N,total P,total K and chlorophyll content were determined. [Result] Cane yield,total biomass yield and sugar content of six varieties were more than 180,200 and 25 t/hm2 respectively,which were higher than that of the control Xintaitang 22. The total nitrogen and total phosphorus contents of six varieties were high and persistent from Jul to Nov,but they decreased sharply in Dec. Total potassium content of all varieties increased from Jul to Dec. Chlorophyll content of all varieties were high from Aug to Oct,but they decreased sharply in Nov. Total nitrogen,total phosphorus and total potassium content of GT02-833,GT97-69 and B9 were higher than that of CK. [Conclusion] GT02-833,GT97-69 and B9 had characteristic of efficient use of nutrients,thus should be extended as elite varieties.

Characteristic Study of a New Sugarcane Variety GT39

[Objective] This study aimed to provide good commercial sugarcane vari-eties for Guangxi sugar industry. [Method] After regional and production test with sugarcane plants in two years and ratoons in one year, a new sugarcane variety GT39 characterized by good ratooning ability, high yield and sugar-content, and large stalks was bred by sexual hybridization between Yuetang 93/159 （male parent） and ROC22 （female parent） through the ＂Five Nurseries＂ breeding procedure, which was approved by Guangxi Crop Variety Approval Committee in March 2012. [Re-sults] The results of regional test showed that the average cane yield of GT39 was 97.2 t/hm2, 5.3% and 11.2% higher than the control ROC22 and ROC16, respective-ly; the average sucrose content was 14.07% from Nov. to next Feb, 0.05% higher than ROC22 and 0.02% lower than ROC16; the average sucrose content was 14.02 t/hm2, 9.1% and 10.4% higher than ROC22 and ROC16, respectively. [Conclusion] GT39 was suited to be planted in both dry land and paddy fields with medium-level or above fertility in Guangxi sugarcane-producing area.

Studies on Membrane Function and Sugar Components of Ultradried Seeds

The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro- viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under -20 degreesC, although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn (Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.

Influences of Surface Drip Irrigation on the Growth, Yield and Quality of Several New Species of Guitang Sugarcane

[Objective] This paper discussed the influences of surface drip irrigation on the growth, yield and quality of several new species of Guitang, in order to provide references for the promotion of new species and high-yield cultivation. [Method] One species is planted in each region, and there were two controls dripping technology or no-dripping technology in each region. [Results] The average yield of dripping-pro- cessed land was 115.91 t/hm2, which was 19.73 t/hm2 higher than the control. The maximum output was GT31, followed by GT34. The output of all Guitang new species was higher than the control ROC22. The average sucrose of dripped sug- arcane was 14.68%, which was 0.19% less than the control of 14.83%. The drip- ping technology was economically beneficial, rising by 15.2% compared with the control. The highest dripping efficiency was GT31, while the lowest one was ROC22. The increasing ratios of drip irrigation efficiency of the tested new Guitang species were higher than ROC22. [Conclusions] Under the drip irrigation condition, the sugarcane yield was extremely higher than the control of non-dripping irrigation, and the economic efficiency was significant. However, the sugar in the sugarcane declined slightly. The sensitivity of several new Guitang species to water was higher than the control ROC22.

Structure and Expression Analyses of a Gene Encoding Fructose-6-Phosphate, 2-Kinase/Fructose-2,6-Bisphosphatase from Maize

A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and designated as mF2KP. The encoded protein is composed of two regions. Its COOH-terminal region is catalytic region and homologous to the enzymes from other eukaryotes; and its NH 2-terminal region is common and special region only in plant. A truncated fragment of mF2KP covering integrated catalytic region was expressed in Escherichia coli. The fusion protein had the activities of fructose-6-phosphate, 2-kinase as well as fructose-2,6-bisphosphatase. Northern blot showed that the transcript level of mF2KP in seedlings initiated from strong-vigor seeds is lower than that from weak-vigor seeds.

Research Progress in Formation and Detection Method of Dextran in Sugarcane Production

Dextran in sugarcane production process is formed by Leuconostoc rnesenteroides. The content levels of dextran is related to sugarcane varieties, field condition （planting pattern, temperature, humidity, sunlight, soil, foreign material）, de- gree of injury （refractory cane, harvesting methods）, and can be rapidly and accu- rately measured by Dextran Immunonephelometric Test Kit. The presence of dextran indicates that sucrose has been lost, so sugarcane dextran is a direct and reliable indicator to measure sugarcane freshness and quality.

Purification and characterization of Moschatin, a novel type Ⅰ ribosomeinactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells

A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of~29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC_(50) of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M_(21) with a IC_(50) of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.

Comparison of New Varieties of Sugarcane in China

This study aims to provide information on the dissemination potentials of some sugarcane varieties in the main sugarcane producing regions of China through screening some newly bred sugarcane varieties. Nine sugarcane varieties were chosen to study their agronomy and yield potentials in comparison with the control vari- ety Roc22. The agronomic characters studied were the sprouting rate, tillering rate, cane production, sugar content, chilling tolerance and sugar production per unit area. Results showed, compared with the control, YT55, Funong15, GT02-901 and YT00- 236 had higher sprouting rate. YT55 and YT00-236 showed higher tillering rate than Roc22. Four varieties, YT55, GT97-69, YT00-236 and Funong15, gave higher cane production than the control. GT02-901 and Y799-21 had the highest sugar content and YT55, Funong15, YT00-236 and GT97-69 produced more sugar per hectare. YZ99-91, YZ03-422, YT55 and YT00-236 showed improved chilling tolerance. It was concluded that YT00-236 and Funong15 are the top two sugarcane varieties with comprehensive advantages and could be widely disseminated through the main sugarcane producing regions in China.

In situ tumor vaccination with adenovirus vectors encoding measles virus fusogenic membrane proteins and cytokines

AIM： To evaluate whether intratumoral expression of measles virus fusogenic membrane glycoproteins H and F （MV-FMG）, encoded by an adenovirus vector Ad.MV-HI F, alone or in combination with local coexpression of cytokines （IL-2, IL-12, IL-18, IL-21 or GM-CSF）, can serve as a platform for inducing tumor-specific immune responses in colon cancer.METHODS： We used confocal laser scanning microscopy and flow cytometry to analyze cell-cell fusion after expression of MV-FMG by dye colocalization. In a syngeneic bilateral subcutaneous MC38 and Colon26 colon cancer model in C57BL/6 and BALB/c mice, we assessed the effect on both the directly vector-treated tumor as well as the contralateral, not directly vector- treated tumor. We assessed the induction of a tumorspecific cytotoxic T lymphocyte （CTL） response with a lactate dehydrogenase （LDH） release assay.RESULTS： We demonstrated in vitro that transduction of MC38 and Colon26 cells with Ad.MV-H/F resulted in dye colocalization, indicative of cell-cell fusion, in addition, in the syngeneic bilateral tumor model we demonstrated a significant regression of the directly vector-inoculated tumor upon intratumoral expression of MV-FMG alone or in combination with the tested cytokines. We observed the highest anti-neoplastic efficacy with MV-FMG and lL-21 coexpression. The degree of tumor regression of the not directly vector-treated tumor correlated with the anti-neoplastic response of the directly vector-treated tumor. This regression was mediated by a tumor-specific CTL response.CONCLUSION： Our data indicate that intratumoral expression of measles virus fusogenic membrane glycoproteins is a promising tool both for direct tumor treatment as well as for tumor vaccination approaches that can be further enhanced by cytokine coexpression.

Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification

AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS.

Effect of chitosan on tobacco mosaic virus(TMV) accumulation, hydrolase activity, and morphological abnormalities of the viral particles in leaves of N. tabacum L. cv. Samsun

The effect of chitosan on the development of infection caused by Tobacco mosaic virus(TMV) in leaves of Nicotiana tabacum L. cv. Samsun has been studied. It was shown that the infectivity and viral coat protein content in leaves inoculated with a mixture of TMV(2 μg/mL) and chitosan(1 mg/mL) were lower in the early period of infection(3 days after inoculation), by 63% and 66% respectively, than in leaves inoculated with TMV only. Treatment of leaves with chitosan 24 h before inoculation with TMV also caused the antiviral effects, but these were less apparent than when the virus and polysaccharide were applied simultaneously. The inhibitory effects of the agent decreased as the infection progressed. Inoculation of leaves with TMV together with chitosan considerably enhanced the activity of hydrolases(proteases, RNases) in the leaves, in comparison with leaves inoculated with TMV alone. Electron microscope assays of phosphotungstic acid(PTA)-stained suspensions from infected tobacco leaves showed that, in addition to the normal TMV particles(18 nm in diameter, 300 nm long), these suspensions contained abnormal(swollen, "thin" and "short") virions. The highest number of abnormal virions was found in suspensions from leaves inoculated with a mixture of TMV and chitosan. Immuno-electron microscopy showed that "thin" virus particles, in contrast to the particles of normal diameter, lost the ability to bind to specific antiserum. It seems that the chitosan-induced activation of hydrolases stimulates the intracellular degradation of TMV particles and hence hydrolase activation may be considered to be one of the polysaccharide-mediated cellular defense mechanisms that limit virus accumulation in cells.

Correlations of the Characteristics of Photosynthates between Female Parent and F_1 Inter-subspecific Hybrids of Rice

The utilization of hybrid rice heterosis between subspecies is an important way to prove rice breeding programs, but the low matter conversion rate of inter- subspecific hybrids becomes the bottleneck to get high yield. To further explore the reasons for the low conversion rate of dry matters, and the coordination mechanism between biological yield＇s potential and grain yield＇s potential, we evaluated the correlations of the agronomic characters between the female parent and F1 inter subspecific hybrids, using a male parent R292, three female parents Y58S, Peiai 64S and 83S, and their hybrid combinations Y58S/292, 64S/292 and 83S/292 as experimental materials, Yueyou 9113 as the control. The results indicated that the contents of both soluble sugar and starch of all the experimental materials varied at different growth stages, and the highest starch content appeared at pre-ripening stage while soluble sugar content was the lowest. Notable positive correlations in a- gronomic characteristics were observed between the female parents and their F1 hybrids. The soluble sugar content in stem was the highest, followed by that in panicle, and the soluble sugar content in sheath and flag leaf was lower. The re- sults provide a theoretical basis for the research on heterosis and yield coordination mechanisms of inter-subspecific hybrids of rice.

种糖

自从偶尔掏了一次蜂窝，尝到了甜甜的蜂蜜，熊儿就深深喜欢上了甜的东西。但是，也不好意思总去掏人家蜜蜂辛辛苦苦酿造的蜂蜜啊，自己又不会酿蜜，可怎么办好？为这，熊儿整天愁眉不展。

Confectionery Hybrid Sunflower Breeding in Russia

Confectionery sunflower hybrid breeding program started at VNIIMK in 1999. Aim of our study was to evaluate sunflower lines and hybrids, developed in this program. Best restorer lines were crossed with two lines with cytoplasmic male sterility （CMS-lines） used as testers. Hybrids were tested for seed yield, husk content and 1,000 seeds weight. Two-year hybrid trial allows us to define the most prominent hybrid combination （VK-905 A ~ K-4） and parental lines （CMS-line VK-905 A and restorer lines K-4, K-3 and K-I） for the future sunflower breeding work. It was further showed that confectionery hybrids with CMS-line VK-905 A do not show decrease in seed size with the increase in plant density.