[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before head...[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant.展开更多
【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture c...【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.展开更多
Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of ...Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of —NH2 and —OH groups increased and fluoride decreased on the membrane surface after modification. Using this kind of affinity membrane, the effects of operation parameters such as pH, ionic strength and flow rate, on the amount of endotoxin removed were investigated. The results showed that the equilibrium adsorption capacity and the dissociation constant of the affinity membrane to endotoxin were 21.4 EU·mg-1 membrane and 0.50EU·ml-1, respectively, at pH 7.0 and ionic strength 0.2 mol·L-1. Adsorption appeared to follow a typical Langmuir adsorption isotherm. At pH 5.0, ionic strength of 0.2 mol·L-1, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6% (11.50 EU·mg-1 membrane), and the recovery of BSA was 93.4% (0.187mg·mg-1 membrane), while at pH 11.0, ionic strength of 0.2mol·L-1, the removal rate of endotoxin from lysozyme solution was 72.4% (9.92EU·mg-1 membrane), and the recovery of lysozyme was 92.3% (0.104 mg·mg-1 membrane).展开更多
[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,...[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,and liquid ratio on the extraction rate of polysaccharides from chrysanthemum were studied.The optimum operation condition by microwave extraction method was determined and was compared with that by traditional extraction method.[Result] The optimum extraction conditions were:800 W,1:15(solid-liquid ratio),and 15 min.The polysaccharides extraction yield under such optimum condition was 5.59%.The extraction yield of impregnation was generally about 2.73%.[Conclusion] Compared with the traditional extraction method,the microwave extraction was simple,convenient,energy saving,and efficient,and was suitable for industrial extraction of polysaccharide.展开更多
Tahini halvah is specific confectionery product known in Bosnia and Herzegovina over centuries. It is made of sesame-seed paste (tahini), sugar and soapwort (Saponaria officinalis) extract by certain technological...Tahini halvah is specific confectionery product known in Bosnia and Herzegovina over centuries. It is made of sesame-seed paste (tahini), sugar and soapwort (Saponaria officinalis) extract by certain technological process, with creating needle-like particles which give a specific fiber structure to halvah. Production of tahini halvah in Bosnia and Herzegovina mostly was placed under artisanal conditions by poorly trained staff and was based on experiences and established practice. There is a very few scientific papers deal with ways of production and specific properties of this product in Bosnia and Herzegovina. Main objective of this paper was focused on studying technological process of 4 different samples of tahini halvah (standard tahini halvah, tahini halvah with pistachios, tahini halvah with cacao and black halvah with wheat grits, cacao and nuts). All samples were produced under industrial conditions. Chemical and sensory quality parameters of investigated samples were presented in this paper. Significant differences between samples were found and influenced by different ingredients and technological process.展开更多
DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. MLSS, and 28.2 -43.8 μg/g soil respectively. The r...DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. MLSS, and 28.2 -43.8 μg/g soil respectively. The resulting DNA is suitable for PCR. By studied methods, higher quality and quantity of sludge DNA could be obtained rapidly and inexpensively from large number of samples, and the PCR product obtained from this protocol was not affected by contaminated higher concentration of heavy metals.展开更多
ZnO nanorods, with the c-axis orientation used for transparent conductors, solar cells, sensors especially the functionalized ZnO nanorods with some kinds of enzymes have been used for biosensor. In this work, we desc...ZnO nanorods, with the c-axis orientation used for transparent conductors, solar cells, sensors especially the functionalized ZnO nanorods with some kinds of enzymes have been used for biosensor. In this work, we describe the process immobilization of galactose oxidase on ZnO nanorods surface with glutaraldehyde as a cross-linker molecule to make the working electrode in electrochemical biosensor. ZnO nanorods were grown on FTO (Fluorine-doped tin oxide) substrate by solution method at low temperature. The crystalline phase and orientation of ZnO nanorods were identified using X-ray diffraction. The efficiency of the immobilization was calculated by Braford method showed that about 36% enzyme content was immobilized on ZnO nanorods surface. The working electrode based on the immobilized ZnO nanorods was tested in galactose solution by CV (cyclic voltammetry) method indicated the value of current intensity is about 0.14 μA. These results clearly demonstrate the potential of galactose sensor based on ZnO nanorod.展开更多
[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and thr...[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and throe-levels was carried out for optimizing the extraction process of polysacchafides from Tegillarca granosa. A central composite des(gn including independent variables, such as extraction temperature (A), extraction time (B), and ethanol concentration (C) was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysacchadde yield. [ Result] The independent variable with the largest effect on response was ethanol concentration (C). The optimum extraction conditions were found to be extraction temperature 69.6℃, extraction time 6.2 h, and ethanol concen- tration of 78% (V/V), respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1. 635%. [ Coaclusioa] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.展开更多
The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composit...The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.展开更多
Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulati...Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulation, respectively. However, the strain Clostridium pasteurianum NRRL B598 showed diverse pattern of solvents production when butanol production started during exponential growth phase in batch cultivation. If cultivated under glucose limited conditions with dilution rate 0.03 hl, constant product concentration was almost achieved. By contrast, glucose non-limited continuous experiment operated with dilution rate 0.07 h-1 lead to mutually adverse oscillations of product and glucose concentrations. Regarding the process economy, glucose limited continuous cultivation seems to be superior due to utilization of total supplied glucose amount, advantageous butanol/acetone ratio and higher butanol yield.展开更多
Using Undaria pinnatifida juice and soya bean as main material, glucono delta lactone (GDL) as coagulant, we produced light green and very nourishing Undaria pinnatifida bean curd. Using the single factor experiment...Using Undaria pinnatifida juice and soya bean as main material, glucono delta lactone (GDL) as coagulant, we produced light green and very nourishing Undaria pinnatifida bean curd. Using the single factor experiment to determine the quantity and concentration of Undaria pinnatifida juice milk, process conditions of coagulation is determined by three factors three levels experiment, the best formulation of Undaria pinnatifida tofu is determined by test of four factors and three levels, process conditions are: the addition of Undaria pinnatifida is 20%, soya-bean milk concentration is1:4.5, the amount of GDL is0.30%, solidification time is 15min, solidification temperature is 95 ℃.展开更多
基金Supported by Special Program for Technology Innovation from Yibin Municipal Bureau of Science(No.200905105)~~
文摘[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant.
文摘【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.
基金Supported by the National Basic Research Program of China (No. 2003CB615706)
文摘Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of —NH2 and —OH groups increased and fluoride decreased on the membrane surface after modification. Using this kind of affinity membrane, the effects of operation parameters such as pH, ionic strength and flow rate, on the amount of endotoxin removed were investigated. The results showed that the equilibrium adsorption capacity and the dissociation constant of the affinity membrane to endotoxin were 21.4 EU·mg-1 membrane and 0.50EU·ml-1, respectively, at pH 7.0 and ionic strength 0.2 mol·L-1. Adsorption appeared to follow a typical Langmuir adsorption isotherm. At pH 5.0, ionic strength of 0.2 mol·L-1, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6% (11.50 EU·mg-1 membrane), and the recovery of BSA was 93.4% (0.187mg·mg-1 membrane), while at pH 11.0, ionic strength of 0.2mol·L-1, the removal rate of endotoxin from lysozyme solution was 72.4% (9.92EU·mg-1 membrane), and the recovery of lysozyme was 92.3% (0.104 mg·mg-1 membrane).
基金Supported by Doctoral Initial Funding of Henan University of TCM(BSJJ2009-34)
文摘[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,and liquid ratio on the extraction rate of polysaccharides from chrysanthemum were studied.The optimum operation condition by microwave extraction method was determined and was compared with that by traditional extraction method.[Result] The optimum extraction conditions were:800 W,1:15(solid-liquid ratio),and 15 min.The polysaccharides extraction yield under such optimum condition was 5.59%.The extraction yield of impregnation was generally about 2.73%.[Conclusion] Compared with the traditional extraction method,the microwave extraction was simple,convenient,energy saving,and efficient,and was suitable for industrial extraction of polysaccharide.
文摘Tahini halvah is specific confectionery product known in Bosnia and Herzegovina over centuries. It is made of sesame-seed paste (tahini), sugar and soapwort (Saponaria officinalis) extract by certain technological process, with creating needle-like particles which give a specific fiber structure to halvah. Production of tahini halvah in Bosnia and Herzegovina mostly was placed under artisanal conditions by poorly trained staff and was based on experiences and established practice. There is a very few scientific papers deal with ways of production and specific properties of this product in Bosnia and Herzegovina. Main objective of this paper was focused on studying technological process of 4 different samples of tahini halvah (standard tahini halvah, tahini halvah with pistachios, tahini halvah with cacao and black halvah with wheat grits, cacao and nuts). All samples were produced under industrial conditions. Chemical and sensory quality parameters of investigated samples were presented in this paper. Significant differences between samples were found and influenced by different ingredients and technological process.
文摘DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. MLSS, and 28.2 -43.8 μg/g soil respectively. The resulting DNA is suitable for PCR. By studied methods, higher quality and quantity of sludge DNA could be obtained rapidly and inexpensively from large number of samples, and the PCR product obtained from this protocol was not affected by contaminated higher concentration of heavy metals.
文摘ZnO nanorods, with the c-axis orientation used for transparent conductors, solar cells, sensors especially the functionalized ZnO nanorods with some kinds of enzymes have been used for biosensor. In this work, we describe the process immobilization of galactose oxidase on ZnO nanorods surface with glutaraldehyde as a cross-linker molecule to make the working electrode in electrochemical biosensor. ZnO nanorods were grown on FTO (Fluorine-doped tin oxide) substrate by solution method at low temperature. The crystalline phase and orientation of ZnO nanorods were identified using X-ray diffraction. The efficiency of the immobilization was calculated by Braford method showed that about 36% enzyme content was immobilized on ZnO nanorods surface. The working electrode based on the immobilized ZnO nanorods was tested in galactose solution by CV (cyclic voltammetry) method indicated the value of current intensity is about 0.14 μA. These results clearly demonstrate the potential of galactose sensor based on ZnO nanorod.
基金Supported by Key Scientific Research Program of Wannan MedicalCollege ( WK2012Z208)
文摘[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and throe-levels was carried out for optimizing the extraction process of polysacchafides from Tegillarca granosa. A central composite des(gn including independent variables, such as extraction temperature (A), extraction time (B), and ethanol concentration (C) was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysacchadde yield. [ Result] The independent variable with the largest effect on response was ethanol concentration (C). The optimum extraction conditions were found to be extraction temperature 69.6℃, extraction time 6.2 h, and ethanol concen- tration of 78% (V/V), respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1. 635%. [ Coaclusioa] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.
基金supported by the National High Technology Research and Development Program of China (863 Program) (2013AA093003)
文摘The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.
文摘Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulation, respectively. However, the strain Clostridium pasteurianum NRRL B598 showed diverse pattern of solvents production when butanol production started during exponential growth phase in batch cultivation. If cultivated under glucose limited conditions with dilution rate 0.03 hl, constant product concentration was almost achieved. By contrast, glucose non-limited continuous experiment operated with dilution rate 0.07 h-1 lead to mutually adverse oscillations of product and glucose concentrations. Regarding the process economy, glucose limited continuous cultivation seems to be superior due to utilization of total supplied glucose amount, advantageous butanol/acetone ratio and higher butanol yield.
文摘Using Undaria pinnatifida juice and soya bean as main material, glucono delta lactone (GDL) as coagulant, we produced light green and very nourishing Undaria pinnatifida bean curd. Using the single factor experiment to determine the quantity and concentration of Undaria pinnatifida juice milk, process conditions of coagulation is determined by three factors three levels experiment, the best formulation of Undaria pinnatifida tofu is determined by test of four factors and three levels, process conditions are: the addition of Undaria pinnatifida is 20%, soya-bean milk concentration is1:4.5, the amount of GDL is0.30%, solidification time is 15min, solidification temperature is 95 ℃.