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星状风毛菊化学成分研究(Ⅰ) 被引量:13
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作者 贾忠建 巨勇 朱子清 《兰州大学学报(自然科学版)》 CAS CSCD 北大核心 1989年第3期64-67,共4页
从星状风毛菊(Saussurea stella Maxim)中分得六种黄酮化合物,经光谱,化学方法及与标准样品对照分别鉴定其为:洋芹素,金合欢素,山奈素,日本椴甙,山奈素—3—0—α—L—鼠李糖甙,和槲皮素—3—O—α—L—鼠李糖甙.其中金合欢素,日本椴甙... 从星状风毛菊(Saussurea stella Maxim)中分得六种黄酮化合物,经光谱,化学方法及与标准样品对照分别鉴定其为:洋芹素,金合欢素,山奈素,日本椴甙,山奈素—3—0—α—L—鼠李糖甙,和槲皮素—3—O—α—L—鼠李糖甙.其中金合欢素,日本椴甙,山奈素—3—0—α—L—鼠李糖甙是首次从该属中发现的,这对该属植物化学分类将有一定的意义. 展开更多
关键词 星状风毛菊 黄酮 糖试 化学成分
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Research Progress in Formation and Detection Method of Dextran in Sugarcane Production 被引量:1
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作者 徐艳芳 刘桂云 +4 位作者 梁达奉 蚁细苗 柳颖 曾练强 常国炜 《Agricultural Science & Technology》 CAS 2016年第7期1692-1696,共5页
Dextran in sugarcane production process is formed by Leuconostoc rnesenteroides. The content levels of dextran is related to sugarcane varieties, field condition (planting pattern, temperature, humidity, sunlight, so... Dextran in sugarcane production process is formed by Leuconostoc rnesenteroides. The content levels of dextran is related to sugarcane varieties, field condition (planting pattern, temperature, humidity, sunlight, soil, foreign material), de- gree of injury (refractory cane, harvesting methods), and can be rapidly and accu- rately measured by Dextran Immunonephelometric Test Kit. The presence of dextran indicates that sucrose has been lost, so sugarcane dextran is a direct and reliable indicator to measure sugarcane freshness and quality. 展开更多
关键词 Sugarcane production DEXTRAN Sugarcane varieties Sugarcane fresh- ness Dextran Immunonephelometric Test Kit
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Study on the Optimal Extraction Process and Antitumor Effect of Polysaccharides from Hemerocallis fulva 被引量:3
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作者 欧丽兰 余昕 +1 位作者 张椿 朱烨 《Agricultural Science & Technology》 CAS 2016年第7期1687-1691,1696,共6页
[Objective] This study aimed to investigate the optimal extraction process and antitumor effect of polysaccharides from Hemerocallis fulva. [Method] The con- tent of polysaccharides was determined with phenoFsulfuric ... [Objective] This study aimed to investigate the optimal extraction process and antitumor effect of polysaccharides from Hemerocallis fulva. [Method] The con- tent of polysaccharides was determined with phenoFsulfuric acid method. On the basis of single-factor experiment, an L9 (34) orthogonal design was performed with extraction rate of polysaccharides as an indicator to analyze the effects of solid-liq- uid ratio, extraction temperature, extraction time and extraction frequency on polysaccharide extraction and to investigate the optimal extraction process. Kunming mice were injected subcutaneously with sarcoma S180 ceils in the right axilla and in- tragastrically administrated with different doses of polysaccharides to observe antitu- mor rate. The levels of IL-2 and TNF-α in serum of S180 sarcoma-bearing mice were detected by antibody sandwich enzyme-linked inununosorbent assay (ELISA). [Result] The optimal extraction process of polysaccharides from H. fulva was: solid-liq- uid ratio of 1:25 (g/ml), extraction temperature of 80 ℃, extraction time of 2 h, and extraction frequency of three times. Under the optimized conditions, average extrac- tion rate of polysaccharides from H. fulva reached 17.47%. Polysacchandes extract-. ed from H. fulva could inhibit the growth of S180 sarcoma in mice. Antitumor rate in high-dose polysaccharide group (1,60 g/kg) reached 38.54%. The levels of IL-2 and ]NF-a in serum of S180 sarcoma-bearing mice were improved by polysaccharides. [Conclusion] Polysaccharides extracted from H. fulva exhibited strong antitumor activ- ity in vivo, which might be related with the improving effect of polysaccharides on immune function. 展开更多
关键词 Hemerocallis fulva POLYSACCHARIDE Orthogonat design IL-2 TNF-Α
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Hydrogen breath test for the diagnosis of lactose intolerance,is the routine sugar load the best one? 被引量:3
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作者 Fiorenza Argnani Mauro Di Camillo +4 位作者 Vanessa Marinaro Tiziana Foglietta Veronica Avallone Carlo Cannella Piero Vernia 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第40期6204-6207,共4页
AIM: To evaluate the prevalence of lactose intolerance (LI) following a load of 12.5 g in patients diagnosed as high-grade malabsorbers using the hydrogen breath test (HBT)-25.METHODS: Ninety patients showing high-gra... AIM: To evaluate the prevalence of lactose intolerance (LI) following a load of 12.5 g in patients diagnosed as high-grade malabsorbers using the hydrogen breath test (HBT)-25.METHODS: Ninety patients showing high-grade malabsorption at HBT-25 were submitted to a second HBT with a lactose load of 12.5 g.Peak hydrogen production,area under the curve of hydrogen excretion and occurrence of symptoms were recorded.RESULTS: Only 16 patients (17.77%) with positive HBT-25 proved positive at HBT-12.5.Hydrogen production was lower as compared to HBT-25 (peak value 21.55 parts per million (ppm) ± 29.54 SD vs 99.43 ppm ± 40.01 SD; P < 0.001).Symptoms were present in only 13 patients.The absence of symptoms during the high-dose test has a high negative predictive value (0.84) for a negative low-dose test.The presence of symptoms during the first test was not useful for predicting a positive low-dose test (positive predictive value 0.06-0.31).CONCLUSION: Most patients with a positive HBT-25 normally absorb a lower dose of lactose and a strict lactose restriction on the basis of a "standard" HBT is,in most instances,unnecessary.Thus,the 25 g lactose tolerance test should probably be substituted by the 12.5 g test in the diagnosis of LI,and in providing dietary guidelines to patients with suspected lactose malabsorption/intolerance. 展开更多
关键词 Lactase deficiency Lactose malabsorption Lactose intolerance Hydrogen breath test
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Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ 11 dextranase mouthwash 被引量:2
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作者 任伟 王淑军 +4 位作者 吕明生 王小贝 房耀维 焦豫良 胡建恩 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2016年第2期354-366,共13页
We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans, which is commonly... We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans, which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQll dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of ZnSO4, lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO4, 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofilm formation inhibition reached 84.49%. In addition, microscopic observation of the biofilm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products. 展开更多
关键词 antimicrobial agent marine dextranase mouthwash response surface methodology BIOFILM scanning electron microscopy confocal laser scanning microscopy
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Preparation of Recombinant Islet Cell Autoantigen 69 kD Fusion Protein
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作者 黄鹤 甘一如 +1 位作者 黄乃萍 张镜宇 《Transactions of Tianjin University》 EI CAS 2002年第4期231-234,共4页
To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screeni... To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screening in type Ⅰ diabetes mellitus, the cDNA fragment of human ICA69 was amplified by PCR, and then cloned into pSPORT 1 vector. After DNA sequencing, it was inserted into pGEX-2T between the sites of EcoR Ⅰ and Sma Ⅰ, then recombinant plasmid p2T-ICA69 was constructed and introduced into E.coli. The GST-ICA69 fusion protein was expressed by the induction of IPTG. The recombinant ICA69 proteins were used to detect the antibodies against hICA69 in 100 healthy subjects and type Ⅰ diabetic serum by the use of indirect ELISA. The sequence analysis showed that the amplified fragments contained 1449 bp, encoded 483 amino acids, and had been correctly inserted into pGEX-2T vector. The recombinant proteins expressed in the prokaryotic cells had immunogenicity and could be used to detect antibodies against ICA69 in type Ⅰ diabetic serum. Finally it can be concluded in this paper that the expression products obtained by the method of gene engineering are recombinant ICA69 antigen and may be used to improve the forecast rate and the diagnostic rate of type Ⅰ diabetes in combination with other tests. 展开更多
关键词 islet cell autoantigen 69 kD protein GST fusion protein IDDM ELISA
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Changes of ghrelin following oral glucose tolerance test in obese children with insulin resistance 被引量:3
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作者 Xiu-Min Wang You-Jun Jiang Li Liang Li-Zhong Du 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第12期1919-1924,共6页
AIM: To characterize changes in ghrelin levels in response to oral glucose tolerance test (OGTT) and to correlate changes in ghrelin levels with changes in insulin and glucose following OGTT in Chinese obese childr... AIM: To characterize changes in ghrelin levels in response to oral glucose tolerance test (OGTT) and to correlate changes in ghrelin levels with changes in insulin and glucose following OGTT in Chinese obese children of Tanner Ⅰ and Ⅱ stage with insulin resistance. METHODS: 22 obese children with insulin resistance state were divided into four groups according to their Tanner stage and gender: boys of Tanner Ⅰ (fir- Ⅰ ), boys of Tanner Ⅱ(BT-Ⅱ ), girls of Tanner Ⅰ (GT- Ⅰ ), girls of Tanner Ⅱ (GT-Ⅱ). Ghrelin, insulin and glucose were measured at 0, 30, 60 and 120 rain following OGTT. The control children with normal BMI were divided into control boys of Tanner Ⅰ (CBT- Ⅰ, n = 6), control boys of Tanner Ⅱ (CBT-Ⅱ, n = 5), control girls of Tanner Ⅰ (CGT- Ⅰ, n = 6), control girls of Tanner Ⅱ (CGT-Ⅱ, n = 5). Fasting serum ghrelin levels were analyzed. RESULTS: Ghrelin levels were lower in obese groups. Ghrelin levels of control group decreased in Tanner Ⅱ stage (CGT- Ⅰ vs CGT-Ⅱ t = -4.703, P = 0.001; CBT- Ⅰ vs CBT- Ⅱ t = -4.794, P = 0.001). Basal ghrelin levels in fir-Ⅱ decreased more significantly than that in BT- Ⅰ group (t = 2.547, P = 0.029). Ghrelin levels expressed a downward trend after OGTT among obese children. The decrease in ghrelin levels at 60 min with respect to basal values was 56.9% in BT- Ⅰ. Ghrelin concentrations at 0 min correlated directly with glucose level at 0 min in fir- Ⅰ (r = 0.898, P = 0.015). There wasn't a significant correlation of ghrelin changes with glucose changes and insulin changes during OGTT in obese children with insulin resistance. CONCLUSION: In conclusion, in obese children with insulin resistance, ghrelin levels decreased with advancing pubertal stage. Ghrelin secretion suppression following OGTT was influenced by gender and pubertal stage. Baseline ghrelin levels and ghrelin suppression after OGTT did not significantly correlate with the degree of insulin resistance and insulin sensitivity. 展开更多
关键词 GHRELIN Oral glucose tolerance test INSULINRESISTANCE Obese children
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Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro 被引量:2
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作者 许勤勤 刘振华 +3 位作者 孙一晟 丁忠杰 吕龙贤 李永泉 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2012年第2期294-301,共8页
Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the fi... Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms. 展开更多
关键词 antioxidant activity Cordyceps ophioglossoides L2 intracellular polysaccharide submerged culture optimization
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Effects of Electroacupuncture at Weiwanxiashu and Zusanli Points on Blood Glucose and Plasma Pancreatic Glucagon Contents in Diabetic Rabbits 被引量:2
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作者 曾志勇 李永义 王友京 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2002年第2期134-136,共3页
The Effects of electroacupuncture (EA) at Weiwanxiashu (EX-B3) and Zusanli (ST 36) points on blood glucose (BG) and plasma pancreatic glucagon (PG) contents were dynamically observed in diabetic rabbits induced by All... The Effects of electroacupuncture (EA) at Weiwanxiashu (EX-B3) and Zusanli (ST 36) points on blood glucose (BG) and plasma pancreatic glucagon (PG) contents were dynamically observed in diabetic rabbits induced by Alloxan. It is found that acupuncture at Weiwanxiashu point can significantly lower the BG content and inhibit release of PG; no significant changes in BG and PG are found when acupuncture is given at Zusanli (ST 36) point alone, however BG and PG contents decrease more obviously when acupuncture employed at both Zusanli and Weiwanxiashu, suggesting that Zusanli has a marked synergetic action with Weiwanxiashu. 展开更多
关键词 ELECTROACUPUNCTURE Acupuncture Points Animals Blood Glucose Diabetes Mellitus Experimental Female GLUCAGON Male RABBITS
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LIPOPOLYSACCHARIDE-INDUCED CYTOTOXICITY AGAINST CULTURED MOUSE HEPATOCYTES AND THE ROLE OF NONPARENCHYMAL LIVER CELLS
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作者 王宇明 HirooOhnishi +1 位作者 YasutoshiMuto 刘沛 《Chinese Medical Sciences Journal》 CAS CSCD 1994年第3期167-170,共4页
Lipopolysaccharide (LPS) was found to induce significant hepatocytotoxicity against cultured mouse hepatocytes. Degeneration and necrosis of cultured hepatocytes and decrease of hepatocyte viability were prominent. T... Lipopolysaccharide (LPS) was found to induce significant hepatocytotoxicity against cultured mouse hepatocytes. Degeneration and necrosis of cultured hepatocytes and decrease of hepatocyte viability were prominent. The aspartate transferase level and 3H-TdR release in the medium were significantly increased after treatment, and the degree of these changes paralleled with LPS concentration. Various other parameters showed no significant difference between the hepatocytes cultured alone and those cocultured with nonparenchymal liver cells. However, if the nonparenchymal liver cells were isolated from mice which had been pretreated with D-galactosamine (GalN) not only was the hepatocyotoxicity induced by LPS enhanced, but the cells also showed certain cytotoxicity against cultured hepatocytes even without LPS, These results suggest that nonparenchymal liver cells might promote LPL-induced hepatocyte injury. 展开更多
关键词 lipopolysaccharide (LPS) cultured hepatocyte CYTOTOXICITY
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Biosorption of Chromium from Aqueous Solution Using Chitosan 被引量:4
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作者 Naoki Kano Kunlhlro Tanabe +2 位作者 Meiling Pang Yanling Deng Hiroshi Imaizumi 《Journal of Chemistry and Chemical Engineering》 2014年第11期1049-1058,共10页
Adsorption experiment from aqueous solutions containing known amount of Cr (chromium) using chitosan was explored to evaluate the efficiency of chitosan as sorbent for Cr. Some variable parameters such as pH, reacti... Adsorption experiment from aqueous solutions containing known amount of Cr (chromium) using chitosan was explored to evaluate the efficiency of chitosan as sorbent for Cr. Some variable parameters such as pH, reaction time and chitosan dosage were optimized. Under the optimum experiment condition, the effect of common ions on the adsorption of Cr (i.e., Na^+, K^+, Mg^2+, Ca^2+ for Cr(Ⅲ), and Cl^-, NO3^-, SO4^2- for Cr(Ⅵ)) was also investigated. Furthermore, the sorption mechanism of Cr by chitosan was investigated by applying Langmuir and Freundlich isotherm equations to the data obtained. In addition, to confirm the characteristics of the chitosan, the surface morphology of the biomass was determined by SEM (scanning electron microscope) and specific surface area analyzer (N2-BET (Brunaeur, Emmet and Teller)). Consequently, the following matters have been mainly clarified: (1) chitosan can be an efficient sorbent for Cr (both Cr(Ⅲ) and Cr(Ⅵ)); (2) the effect of common ions on the adsorption ofCr (Cr(Ⅲ) and Cr(Ⅵ)) was not significant under this experimental conditions; (3) adsorption isotherms using the chitosan can be generally described by Langmuir isotherm more satisfactorily for Cr. The adsorption may have occurred mainly by monolayer reaction; (4) the rates of adsorption were found to conform to pseudo-second order kinetics. 展开更多
关键词 BIOSORPTION CHROMIUM CHITOSAN adsorption isotherms KINETICS
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Exploring valid internal-control genes in Porphyra yezoensis(Bangiaceae) during stress response conditions 被引量:3
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作者 王文磊 吴晓洁 +5 位作者 王超 贾兆君 何林文 韦一凡 牛建峰 王广策 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第4期783-791,共9页
To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test ... To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species responding the stress conditions in the intertidal. Absolute real-time PCR technology was applied to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species in stress responding. According to the results of photosynthesis parameters, we observed that Y(II) and Fv/Fm were significantly affected when stress was imposed on the thalli of Porphyra yezoensis, but underwent almost completely recovered under normal conditions, which were collected for the following experiments. Then three samples, which were treated with different grade stresses combined with salinity, irradiation and temperature, were collected. The transcription numbers of seven constitutive expression genes in above samples were determined after RNA extraction and cDNA synthesis. Finally, a general insight into the selection of internal control genes during stress response was obtained. We found that there were no obvious effects in terms of salinity stress (at salinity 90) on transcription of most genes used in the study. The 18S ribosomal RNA gene had the highest expression level, varying remarkably among different tested groups. RPS8 expression showed a high irregular variance between samples. GAPDH presented comparatively stable expression and could thus be selected as the internal control. EF-la showed stable expression during the series of multiple-stress tests. Our research provided available references for the selection of internal control genes for transcripts determination of P. yezoensis. 展开更多
关键词 constitutive expression gene housekeeping gene Porphyra yezoensis Ueda real-timequantitative PCR stress responding
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Design and activity evaluation of deoxyribozymes specifically targeting hepatitis C virus RNA
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作者 于乐成 王宇明 +4 位作者 王升启 顾长海 毛青 陈忠斌 刘鸿凌 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第3期143-149,共7页
Objective: To explore the cleaving and inhibitory activity of hepatitis C virus ( HCV) -specific deoxyri-bozymes (DRz) at both molecular and transgeneic cellular levels. Methods: According to the secondary structure o... Objective: To explore the cleaving and inhibitory activity of hepatitis C virus ( HCV) -specific deoxyri-bozymes (DRz) at both molecular and transgeneic cellular levels. Methods: According to the secondary structure of HCV 5'-noncoding region (5'-NCR) and the sites characterized with 5'…Y ↓ R…3'(Y = A/G,R = U/C) , HCV-spe-cific naive deoxyribozymes were designed and named DRz-232, DRz-127, DRz-84, DRz1, and the phosphorothioate deoxyribozymes (PSDRz) and mutated phosphorothioate deoxyribozymes (MPSDRz) were also designed. HCV RNA 5'-NCR was transcribed in vitro from linearized plasmid pHCV-neo and radiolabelled at its 5'-end. DRz, PSDRz or MPSDRz was respectively mixed with the substrate RNA and incubated under appropriate conditions, the cleaved products were displayed by 8% denaturated polyacrylamide gel electrophoresis (PAGE) and autoradiography, and the optical density of each band was measured to calculate cleavage rates. After that, every kind of DRz was added respectively to the cultured transgeneic HepG2 cells containing luciferase gene controlled by HCV 5'-NCR. The cells were lysed at intended time points and the activity of luciferase was measured with chemiluminescence method for calculating inhibition rates. Results: After incubated for 90 min in vitro, the cleavage rates of DRz-127, PSDRz-127, DRz1 and PS-DRz1 reached 32.6% , 30. 8% , 24. 3% and 21. 5% , respectively. No cleavage product was observed in any MPSDRz. DRz-127, PSDRz-127, DRz1 and PSDRzl had an inhibitory rate of 53. 2% , 50. 6% , 44. 7% and 43. 3% respectively in transgeneic HepG2 cells in the first 24 h when the final dose of the DRz was 0. 5μmol/L, higher than that of the corresponding MPSDRz. There was no significant difference between the inhibitory effect of each DRz and its PSDRz in HepG2 cells, but the inhibitory rate of DRz decreased more rapidly than that of the latter with the elapse of time. The results from transfection groups were significantly better than those of non-transfection groups. Conclusion: Rationally-designed HCV-specific deoxyribozymes are able to cleave target RNA at molecular level in vitro, and efficiently inhibit the expression of luciferase gene controlled by HCV 5'-NCR in transgeneic cells. Appropriate PSDRz may be more stable, and thus more suitable than the naive DRz in the application to cells. Introduction of the deoxyribozymes with transfection is more efficient than with direct delivering ways. 展开更多
关键词 deoxyribozymes hepatitis C virus in vitro transcription transgeneic therapy
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In vitro corrosion behavior and cytotoxicity property of magnesium matrix composite with chitosan coating 被引量:1
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作者 戴翌龙 余琨 +3 位作者 陈良建 陈畅 乔雪岩 颜阳 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第3期829-834,共6页
Mg-6%Zn-10%β-Ca3(PO4)2 composite was prepared through powder metallurgy methods with different chitosan coatings on its surface. The properties of the chitosan coatings on the surface of Mg-6%Zn-10%β-Ca3(PO4)2 compo... Mg-6%Zn-10%β-Ca3(PO4)2 composite was prepared through powder metallurgy methods with different chitosan coatings on its surface. The properties of the chitosan coatings on the surface of Mg-6%Zn-10%β-Ca3(PO4)2 composite, such as the adhesion ability, the corrosion behavior and the cytotoxicity properties, were investigated, and the microstructure of the chitosan coating was observed by scanning electron microscope(SEM). The results show that chitosan coating improves the corrosion resistance of the magnesium composite specimens significantly. Mg-6%Zn-10%β-Ca3(PO4)2 composite specimens exhibit good corrosion resistance and low p H values in simulated body fluid(SBF) at 37 °C in the immersion test with 7-layer chitosan coating whose relative molecular mass is 30×104 Da. The cytotoxicity tests indicate that Mg-6%Zn-10%β-Ca3(PO4)2 with chitosan coating is nontoxic with a cytotoxicity grade of zero against L-929 cells, which is better than that of uncoated composites. 展开更多
关键词 magnesium matrix composite chitosan coating in vitro test cytotoxicity
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Hesperidin Reduces Cisplatin-lnduced DNA Damage in Bone Marrow Cells of Mice
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作者 Tatiane da Silva Passos Esdras Andrade Santana +3 位作者 Maina Mantovanelli da Mota Jean Carlos Vencioneck Dutra Juliana Macedo Delarmelina Maria do Carmo Pimentel Batitucci 《Journal of Pharmacy and Pharmacology》 2017年第5期282-288,共7页
Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA (deoxyribonucleic acid) damage, however there are few reports about hesperidin effects against cisplati... Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA (deoxyribonucleic acid) damage, however there are few reports about hesperidin effects against cisplatin-DNA damage induction. The aim of this work was to evaluate hesperidin antimutagenicity against cisptatin-DNA damage. (1) The antimutagenicity of hesperidin was assayed by bone marrow of mice in vivo using the micronucleus test. Hesperidin pre-treatment protocol reduced the frequency of MNPCE (micronucleated polychromatic erythrocytes) and the dose of 100 mg·kg-1 was highest efficiency, with 65.24% of damage reduction. In the simultaneous treatment protocol, the dose of 200 mg·kg-1 exhibited a more effective reduction of MNPCE, with 94.01% of damage reduction. (2) Hesperidin was also effective in reducing the MNPCE frequency in the post-treatment protocol for all doses, with 77.48%, 82.13% and 90.08% of damage reduction at the doses of 100, 200 and 400 mg·kg-1, respectively. From the study, it can be concluded that hesperidin was able to promote the reduction of micronuclei frequency and DNA damage induced by cisplatin. Hesperidin is a powerful antioxidant compound and its chemoprotective effects on DNA may occur due to its association with the antioxidant cell system which is responsible for eliminate free radicals generated by chemical harmful to DNA. 展开更多
关键词 HESPERIDIN micronucleus test DNA damage CHEMOPROTECTION antimutagenicity.
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IDENTIFICATION OF GLUCOSE TRANSPORTER- 1 AND ITS FUNCTIONAL ASSAY IN MOUSE GLOMERULAR MESANGIAL CELLS CULTURED IN VITRO
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作者 章精 刘志红 +1 位作者 刘栋 黎磊石 《Chinese Medical Sciences Journal》 CAS CSCD 2001年第1期35-39,共5页
To evaluate the role of glucose transporter- l (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was dete... To evaluate the role of glucose transporter- l (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT- PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2- deoxy- [3H]- D- glucose uptake. Results. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2- deoxy- D- glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. Conclusion. Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells. 展开更多
关键词 glucose transporter- 1 mesangial cells PHLORETIN diabetic nephropathy
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Influence of Cutting Stages and Sugarcane Varieties on Sucrose Content in a Brazilian Power Plant with Mechanized Harvesting
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作者 Ricardo Ghantous Cervi Sergio Augusto Rodrigues +1 位作者 Ernee Kozyreff Filho Paulo Andre de Oliveira 《Journal of Agricultural Science and Technology(B)》 2018年第4期220-227,共8页
This study, carried out in a sugarcane mill located in the center-west region of Sao Paulo state, Brazil, aimed to evaluate the association of sucrose amount (POL%) with sugarcane varieties and cutting stages using ... This study, carried out in a sugarcane mill located in the center-west region of Sao Paulo state, Brazil, aimed to evaluate the association of sucrose amount (POL%) with sugarcane varieties and cutting stages using mechanized harvesting. A two-way analysis of variance (ANOVA), complemented by multiple comparison tests, was used in order to identify the effects of variety types and cutting stages on the sugarcane POL% content. An interaction between the sugarcane variety and the cutting stage was observed; therefore, both two factors cannot be independently evaluated regarding POL%. In addition, it was found that the sucrose amount from the plant production in the evaluated period presented significant differences between the cutting stages only for one variety, namely CTC2, which was different from the others in most of the cutting stages. Considering the observed results, it can be concluded that analyzing sucrose amount statistically in the mill production can allow producers to posteriorly better monitor information on the sugarcane varieties harvested under mechanized processes. 展开更多
关键词 Mechanized harvesting sugarcane cultivation sugarcane POL%.
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Efficacy and safety of saxagliptin in patients with type 2 diabetes mellitus:a meta-analysis of randomized controlled trials 被引量:2
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作者 姚莉 范芳芳 +2 位作者 胡兰 赵生俊 郑丽丽 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2016年第2期128-139,共12页
As a new oral hypoglycemic agent, saxagliptin belongs to the class of dipeptidyl peptidase-4 (DPP-4) inhibitors. However, it remains inconclusive whether saxagliptin is associated with increased risk of adverse even... As a new oral hypoglycemic agent, saxagliptin belongs to the class of dipeptidyl peptidase-4 (DPP-4) inhibitors. However, it remains inconclusive whether saxagliptin is associated with increased risk of adverse events (AE) and efficacy as add-on treatment. Therefore, we performed an up-to-date meta-analysis to compare the efficacy and safety of saxagliptin with placebo and other oral hypoglycemic agents in adult patients with type 2 diabetes mellitus (T2DM). Randomized clinical trials (RCTs) comparing saxagliptin with comparators were retrieved by selecting articles from Pubmed, Embase, Cochrane Library and Clinical Trials Registry Platform up to Oct. 2013. Weighted mean difference (WMD) was used to analyze the effect of hypoglycemic agents on HbAlc, weight and fasting plasma glucose (FPG). While the patients who achieved HbAlc〈7.0% and had AE were analyzed as relative risks (RR). A total of 18 articles from 16 RCTs and one clinic trial from the WHO International Clinical Trials Registry Platform met the included criterion. Clinically significant decrease from baseline HbAlc compared with placebo was certified for 2.5 mg/day saxagliptin (WMD = -0.45%, 95% CI, -0.48% to -0.42%) and 5 mg/d saxagliptin (WMD = -0.52%, 95% CI, -0.60% to -0.44%). Saxagliptin as add-on therapy was superior to thiazolidinediones, up-titrated glyburide, up-titrated metformin or metformin monotherapy in achieving HbA1c〈7.0%. Treatment with saxagliptin had negligible effect on weight, and it was considered weight neutral. Saxagliptin treatment did not increase the risk of hypoglycemia (RR = 1.28, 95% CI 0.72 to 2.27, P = 0.40) and serious adverse experiences (RR = 1.25, 95% CI 0.94 to 1.66, P = 0.13). No statistically significant differences were observed between saxagliptin and comparators in terms of the risk of infections. The present study showed that saxagliptin was effective in improving glycaemic control in T2DM with a low risk of hypoglycaemia and incidence of infections in either monotherapy or add-on treatment. This founding should be further certified by large-sample size and good-designed RCT. 展开更多
关键词 DPP-4 inhibitor SAXAGLIPTIN META-ANALYSIS Type 2 diabetes mellitus Fasting plasma glucose Glycosylated hemoglobin Randomized controlled trial HYPOGLYCAEMIA
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Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia 被引量:1
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作者 唐松山 张娟辉 +2 位作者 刘换新 周东 祁荣 《Neuroscience Bulletin》 SCIE CAS CSCD 2009年第1期7-14,共8页
Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5(RGC-5) cells,pro-protein convertase-2(PC2),carboxypeptidase-E(CPE) and preproneuropeptide Y(prep... Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5(RGC-5) cells,pro-protein convertase-2(PC2),carboxypeptidase-E(CPE) and preproneuropeptide Y(preproNPY) protein levels in the ischemic RGC-5 cells and conditioned medium were analyzed. Methods The RGC-5 cell was differentiated in 0.1 μmol/L staurosporine for 24 h and then stressed by different doses of oxygen and glucose deprivation(OGD). The acute or chronic OGD-induced cell death rates w... 展开更多
关键词 neuropeptide pro-protein processing system retina ganglion cell-5 in vitro oxygen and glucose deprivation pro-protein convertase-2 carboxypeptidase-E preproneuropeptide-Y
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Effect of curcumin on rats/mice with diabetic nephropathy: a systematic review and Meta-analysis of randomized controlled trials 被引量:9
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作者 Wei Wu Hua Geng +2 位作者 Zhaoru Liu Hong Li Zhangzhi Zhu 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2014年第4期419-429,共11页
OBJECTIVE: To assess the renal protective effects of curcumin administration ondiabetic rats/mice.METHODS: Databases were searched electronically and conference papers searched manually for search terms to find releva... OBJECTIVE: To assess the renal protective effects of curcumin administration ondiabetic rats/mice.METHODS: Databases were searched electronically and conference papers searched manually for search terms to find relevant studies. Articles were assessed independently by two reviewers. Review Manager 5.1 was used fordata analysis.RESULTS: Fourteen randomized controlled experiments were included. Meta-analysis demonstrated that blood sugar levels and kidney weight to body weight ratios in the model group were higher than those in the normal group, and the curcumin group had significantly lower mesangial area to glomerular area ratios compared with the model group, and also lower levels of urinary protein,blood urea nitrogen and serum creatinine.CONCLUSION: Curcumin shows protective effects on the kidneys of rats/mice with diabetes. 展开更多
关键词 Curcumin Diabetic nephropathies Meta-analysis Review Randomized controlled trials
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