Human tissue kallikreins are a family of 15 trypsin or chymotrypsin- like secreted serine proteases (hK1- hK15). hK5, hK6, hK7, hK8, and hK13 have been identified in the stratum corneum (SC), stratum granulosum, and s...Human tissue kallikreins are a family of 15 trypsin or chymotrypsin- like secreted serine proteases (hK1- hK15). hK5, hK6, hK7, hK8, and hK13 have been identified in the stratum corneum (SC), stratum granulosum, and skin appendages. It has been reported that hK5 and hK7 degrade desmosomes/corneodesmosomes, suggesting that kallikreins are responsible for desquamation. We report the quantification of hK5, hK6, hK7, hK8, hK10, hK11, hK13, and hK14 in the SC by ELISA and their variation among age groups. The total SC trypsin and chymotrypsin-like activities were also measured. The amount of hK7, hK8, and hK11 (ng per mg dry weight) were high, and varied from 6 to 14, hK5 (2.0- 4.0) was present at intermediate levels, and hK10 (0.65- 1.0), hK14 (0.1- 0.3), hK6 (0.1- 0.3), and hK13 (0.02- 0.1) were present at lower levels. hK6 and hK14 were significantly lower in females between 20 and 59 y. hK5, hK7, hK10, hK11, and hK14 were not significantly different across the age groups. hK8 was lowest at extremes of age (highest at 30- 39 y), hK6 was lower at > 30 y, and hK13 was lower at > 20 y. Overall trypsin-like activity did not differ across age groups but was higher in subjects < 11 y. Overall chymotrypsin-like activity was not related to age. In conclusion, we found multiple kallikreins in the SC and suggest that these enzymes may be responsible for desquamation through an enzymatic cascade pathway.展开更多
胃肝样腺癌（hepatoid adenocarcinoma of stomach，HAS）是原发于胃黏膜并且同时具有腺癌和肝细胞癌特征的一种胃癌。病理可同时观察到腺癌区和肝样分化区，因此得名。免疫组织化学染色甲胎蛋白（AFP）、α1抗胰蛋白酶（α1-AAT）、...胃肝样腺癌（hepatoid adenocarcinoma of stomach，HAS）是原发于胃黏膜并且同时具有腺癌和肝细胞癌特征的一种胃癌。病理可同时观察到腺癌区和肝样分化区，因此得名。免疫组织化学染色甲胎蛋白（AFP）、α1抗胰蛋白酶（α1-AAT）、α1抗糜蛋白酶（α1-ACT）阳性，癌胚抗原（CEA）亦可阳性。临床特点以老年男性多见，胃窦部多发，血清AFP可不同程度升高，肝转移率高，分化低，预后明显较普通胃癌差，故作为一种胃癌亚型与一般胃癌区分。展开更多
文摘Human tissue kallikreins are a family of 15 trypsin or chymotrypsin- like secreted serine proteases (hK1- hK15). hK5, hK6, hK7, hK8, and hK13 have been identified in the stratum corneum (SC), stratum granulosum, and skin appendages. It has been reported that hK5 and hK7 degrade desmosomes/corneodesmosomes, suggesting that kallikreins are responsible for desquamation. We report the quantification of hK5, hK6, hK7, hK8, hK10, hK11, hK13, and hK14 in the SC by ELISA and their variation among age groups. The total SC trypsin and chymotrypsin-like activities were also measured. The amount of hK7, hK8, and hK11 (ng per mg dry weight) were high, and varied from 6 to 14, hK5 (2.0- 4.0) was present at intermediate levels, and hK10 (0.65- 1.0), hK14 (0.1- 0.3), hK6 (0.1- 0.3), and hK13 (0.02- 0.1) were present at lower levels. hK6 and hK14 were significantly lower in females between 20 and 59 y. hK5, hK7, hK10, hK11, and hK14 were not significantly different across the age groups. hK8 was lowest at extremes of age (highest at 30- 39 y), hK6 was lower at > 30 y, and hK13 was lower at > 20 y. Overall trypsin-like activity did not differ across age groups but was higher in subjects < 11 y. Overall chymotrypsin-like activity was not related to age. In conclusion, we found multiple kallikreins in the SC and suggest that these enzymes may be responsible for desquamation through an enzymatic cascade pathway.
文摘胃肝样腺癌（hepatoid adenocarcinoma of stomach，HAS）是原发于胃黏膜并且同时具有腺癌和肝细胞癌特征的一种胃癌。病理可同时观察到腺癌区和肝样分化区，因此得名。免疫组织化学染色甲胎蛋白（AFP）、α1抗胰蛋白酶（α1-AAT）、α1抗糜蛋白酶（α1-ACT）阳性，癌胚抗原（CEA）亦可阳性。临床特点以老年男性多见，胃窦部多发，血清AFP可不同程度升高，肝转移率高，分化低，预后明显较普通胃癌差，故作为一种胃癌亚型与一般胃癌区分。
