Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partit...Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partition coefficients and extraction ratio of teicoplanin in three different solvent systems were studied: conventional extraction, reactive extraction and reverse micelle extraction. With conventional solvent extraction, n-butanol demonstrated high partition coefficient for teicoplanin, but low extraction ratio because of its high solubility in water. Reactive extraction of teicoplanin showed the highest partition coefficient with almost 100% recovery in organic phase when tri-n-octylmethyl ammonium chloride (TOMAC) was used as extractant and pH value was above 5.0. A reverse micelle system, consisted of isooctane +10mmol-L-1 TOMAC +1% n-octanol, also offered high separation factor for teicoplanin. The results are beneficial for the design of teicoplanin separation and purification process.展开更多
Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method...Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method is presented in this short paper. This proposed study is significantly different from the conventional pulse tube method because of the capability for a single plane damped sine pulse wave to generate in the water-filled pulse tube with a regular waveform and short duration time of about 1ms. During the generation process of the pulse, an inverse filter principle was adopted to compensate the transducer response. The effect of the characteristics of tube termination can be eliminated through the generation process of the pulse. Reflection coefficient from a water/air interface was measured to verify the proposed method. When compared with the expected theoretical values, a relatively good agreement can be obtained in the low frequency range of 500-2 000 Hz.展开更多
文摘Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partition coefficients and extraction ratio of teicoplanin in three different solvent systems were studied: conventional extraction, reactive extraction and reverse micelle extraction. With conventional solvent extraction, n-butanol demonstrated high partition coefficient for teicoplanin, but low extraction ratio because of its high solubility in water. Reactive extraction of teicoplanin showed the highest partition coefficient with almost 100% recovery in organic phase when tri-n-octylmethyl ammonium chloride (TOMAC) was used as extractant and pH value was above 5.0. A reverse micelle system, consisted of isooctane +10mmol-L-1 TOMAC +1% n-octanol, also offered high separation factor for teicoplanin. The results are beneficial for the design of teicoplanin separation and purification process.
基金Supported by the National Natural Science Foundation of China under Grant No. 11204242 China Postdoctoral Foundation under Grant No. 2011M501477
文摘Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method is presented in this short paper. This proposed study is significantly different from the conventional pulse tube method because of the capability for a single plane damped sine pulse wave to generate in the water-filled pulse tube with a regular waveform and short duration time of about 1ms. During the generation process of the pulse, an inverse filter principle was adopted to compensate the transducer response. The effect of the characteristics of tube termination can be eliminated through the generation process of the pulse. Reflection coefficient from a water/air interface was measured to verify the proposed method. When compared with the expected theoretical values, a relatively good agreement can be obtained in the low frequency range of 500-2 000 Hz.
