The tolerance of a restorer line R1056 of rice and its hybrid combinations to high temperature at flowering stage was evaluated by sowing them at three dif- ferent dates and transplanting them into a greenhouse equipp...The tolerance of a restorer line R1056 of rice and its hybrid combinations to high temperature at flowering stage was evaluated by sowing them at three dif- ferent dates and transplanting them into a greenhouse equipped with heating system at flowering stage. Several important agronomic traits of these rice varieties or combinations were also measured. The results revealed that treated by high temper- ature (38 ℃) at flowering stage, the seed setting rate (70.4%), percentage of dehis- cent anthers (75.5%), and pollen number per floret (78) of R1056 were close to those of N22, which is tolerant to high temperature, and significantly higher than those of the rice varieties sensitive to high temperature. The seed setting rate, per- centage of dehiscent anthers, and pollen number per floret of the hybrid combina- tions generated from R1056 were smaller than those of R1056, but higher than those of the rice varieties sensitive to high temperature. R1056 had excellent agro- nomic traits, and good combining ability, and its hybrid combinations had low plant height, numerous panicles and high yield, suggesting that R1056 is not only an ex- cellent rice variety with tolerance to high temperature, but also an excellent restorer in three-line hybrid rice breeding.展开更多
Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was m...Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding.展开更多
Two hundred and forty recombinant inbred lines (RIL) derived from a cross TD70/Kasalath and its linkage map including 141 SSR markers were used to map QTLs controlling panicle length (PL), total seeds per panicle ...Two hundred and forty recombinant inbred lines (RIL) derived from a cross TD70/Kasalath and its linkage map including 141 SSR markers were used to map QTLs controlling panicle length (PL), total seeds per panicle (TSP) and grain density (GD) in 2010 and 2011. The results showed that a total of 23 QTLs controlling three panicle traits were detected on chromosomes 2, 3, 4, 6, 7, 8 and 10, respec- tively, including 5 QTLs controlling PL, 8 QTLs controlling TSP, 10 QTLs controlling GD, with the LOD value ranging between 2.5-9.3, and the QTLs explained the ob- served phenotypic by 4.0%-20.8%. The marker interval RM5699-RM424 on chro- mosome 2, RM489-RM1278 on chromosome 3, RM3367-RM1018 on chromosome 4, RM3343-RM412 on chromosome 6 were common marker intervals for TSP and GD; six QTLs (qPL3, qTSP4, qTSP6-2, qTSP7, qGD3-2 and qGDT) were detected in two years. Among these QTLs, the qPL3, qTSP6-2, qGD3-2 and qGD7 were major QTLs. All QTLs for PL mapped in the present study had been mapped QTLs previously by other research groups, 16 QTLs controlling TSP and GD were new ones which contributed the observed phenotypic variance range by 4%-9.5%. These results laid a founda^ion for further fine positioning or cloning these QTLs.展开更多
[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions neede...[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS + BA 0.3 mg/L +2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS + AgNO31.5 mg/L + BA 0.4 mg/L + NAA 0.1 mg/L. 1/2 MS + IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.展开更多
[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD ch...[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD changing of agronomic traits was studied by using 24 rice somaclonal and its donor parent under two sowing dates. [Result]The average values of RITSD were higher (over one) for the traits of pant height (PH),density of spikelets (DS),filled spikelets per panicle (FSP),spikelets per panicle (SP) and grain weight per plant (GWP),but lower for days of sowing to heading (DSH),effective panicles (EP),panicle length (PL),seed setting rate (SS) and 1 000-grain weight (1 000-GW). These showed that PH,DS,FSP,SP and GWP were easily affected by sowing date and tended to higher with the later sowing; the RITSD of somaclonal changed with the agronomic traits. The frequency of RITSD' variation of DSH and PH was higher than that of PL. The RITSD' value of EP,PL,DS,FSP,SP and SS became higher,and higher or lower for DSH and GWP in different somaclonal; compared with the donor,20 of 24 was significantly different,and the frequency of RITSD variation was over 83. 3%,however,RITSD of the most lines (75%) changed only for 1-2 characters; cluster analysis showed that the RITSD of agronomic traits belonged to different combinations of somaclonal in rice. [Conclusion]the study had provided a theoretical basis for the screening and using of the somaclonal.展开更多
[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made us...[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities (LN: 90 kg/hm2;MN: 180 kg/hm2;HN: 270 kg/hm2) on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves' function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain's weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.展开更多
A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown p...A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation.展开更多
[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture w...[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture were set to measure their photosynthetic indices, chlorophyll content and root vigor, and to observe their leaf tissue structure and stomatal characteristics. [Result] The findings indicated that there are no differences in the leaf physiological indices between bowl lotus under hydroponics and soil culture, while the leaf stomata of hydroponic bowl lotus is bigger and its amount is larger than that of soil-culture bowl lotus. At the same time, the ratio of the palisade tissue thickness to spongy tissue thickness is small,and its leaf tissue structure is loose. The root vigor of hydroponic bowl lotus reached its summit earlier, then began to drop. Whereas, the root activity of soil-culture lotus sustained increasing, with vigorous growth. [Conclusion] Therefore, it indicated that hydroponic bowl lotus can adapt to the aquatic-culture environment well and quickly, meanwhile, it also enters into its aging period quickly and its growth cycle gets shorter.展开更多
Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single...Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.展开更多
[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Haina...[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.展开更多
基金Supported by the Fund from Agricultural Science and Technology Innovation Center of Hubei Province(2007-620-001-003)Key Project of Natural Science Foundation of Hubei Province(2014CFA103)~~
文摘The tolerance of a restorer line R1056 of rice and its hybrid combinations to high temperature at flowering stage was evaluated by sowing them at three dif- ferent dates and transplanting them into a greenhouse equipped with heating system at flowering stage. Several important agronomic traits of these rice varieties or combinations were also measured. The results revealed that treated by high temper- ature (38 ℃) at flowering stage, the seed setting rate (70.4%), percentage of dehis- cent anthers (75.5%), and pollen number per floret (78) of R1056 were close to those of N22, which is tolerant to high temperature, and significantly higher than those of the rice varieties sensitive to high temperature. The seed setting rate, per- centage of dehiscent anthers, and pollen number per floret of the hybrid combina- tions generated from R1056 were smaller than those of R1056, but higher than those of the rice varieties sensitive to high temperature. R1056 had excellent agro- nomic traits, and good combining ability, and its hybrid combinations had low plant height, numerous panicles and high yield, suggesting that R1056 is not only an ex- cellent rice variety with tolerance to high temperature, but also an excellent restorer in three-line hybrid rice breeding.
基金Supported by the Project of Meteorological Service Website Construction for HainanBreeding(Qiong Nong Ji Cai No.[2013]18 of Hainan Provincial Department of Agriculture)~~
文摘Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding.
基金Supported by Fund for Jiangsu Agricultural Scientific Self-innovation Fund[CX(12)1003]Jiangsu Province Agricultural Science&Technology Support Program(BE2013301)+1 种基金Super Rice Breeding and Demonstration Program of the Ministry of AgricultureSpecial Fund of Modern Agricultural Industry Technology System(CARS-0147)~~
文摘Two hundred and forty recombinant inbred lines (RIL) derived from a cross TD70/Kasalath and its linkage map including 141 SSR markers were used to map QTLs controlling panicle length (PL), total seeds per panicle (TSP) and grain density (GD) in 2010 and 2011. The results showed that a total of 23 QTLs controlling three panicle traits were detected on chromosomes 2, 3, 4, 6, 7, 8 and 10, respec- tively, including 5 QTLs controlling PL, 8 QTLs controlling TSP, 10 QTLs controlling GD, with the LOD value ranging between 2.5-9.3, and the QTLs explained the ob- served phenotypic by 4.0%-20.8%. The marker interval RM5699-RM424 on chro- mosome 2, RM489-RM1278 on chromosome 3, RM3367-RM1018 on chromosome 4, RM3343-RM412 on chromosome 6 were common marker intervals for TSP and GD; six QTLs (qPL3, qTSP4, qTSP6-2, qTSP7, qGD3-2 and qGDT) were detected in two years. Among these QTLs, the qPL3, qTSP6-2, qGD3-2 and qGD7 were major QTLs. All QTLs for PL mapped in the present study had been mapped QTLs previously by other research groups, 16 QTLs controlling TSP and GD were new ones which contributed the observed phenotypic variance range by 4%-9.5%. These results laid a founda^ion for further fine positioning or cloning these QTLs.
文摘[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS + BA 0.3 mg/L +2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS + AgNO31.5 mg/L + BA 0.4 mg/L + NAA 0.1 mg/L. 1/2 MS + IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.
基金Supported by Chongqing Major Research Project "Innovation in Hybrid Rice Breeding and New Materials" ( CSTC, 2007, AA1019 )Sichuan Province Breeding Research Project (06yzgg-01)~~
文摘[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD changing of agronomic traits was studied by using 24 rice somaclonal and its donor parent under two sowing dates. [Result]The average values of RITSD were higher (over one) for the traits of pant height (PH),density of spikelets (DS),filled spikelets per panicle (FSP),spikelets per panicle (SP) and grain weight per plant (GWP),but lower for days of sowing to heading (DSH),effective panicles (EP),panicle length (PL),seed setting rate (SS) and 1 000-grain weight (1 000-GW). These showed that PH,DS,FSP,SP and GWP were easily affected by sowing date and tended to higher with the later sowing; the RITSD of somaclonal changed with the agronomic traits. The frequency of RITSD' variation of DSH and PH was higher than that of PL. The RITSD' value of EP,PL,DS,FSP,SP and SS became higher,and higher or lower for DSH and GWP in different somaclonal; compared with the donor,20 of 24 was significantly different,and the frequency of RITSD variation was over 83. 3%,however,RITSD of the most lines (75%) changed only for 1-2 characters; cluster analysis showed that the RITSD of agronomic traits belonged to different combinations of somaclonal in rice. [Conclusion]the study had provided a theoretical basis for the screening and using of the somaclonal.
基金Supported by Special Scientific Research Fund of Agricultural Public Welfare Profession(200903008-09)~~
文摘[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities (LN: 90 kg/hm2;MN: 180 kg/hm2;HN: 270 kg/hm2) on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves' function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain's weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.
文摘A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation.
基金Supported by Key Scientific and Technological Project of Henan Province(072102150001)~~
文摘[Objective] The experiment aimed to study the growth characteristics of hydroponic bowl lotus. [Method] The lotus variety Hongxia was chosen as the experimental material. Two treatments, hydroponics and soil culture were set to measure their photosynthetic indices, chlorophyll content and root vigor, and to observe their leaf tissue structure and stomatal characteristics. [Result] The findings indicated that there are no differences in the leaf physiological indices between bowl lotus under hydroponics and soil culture, while the leaf stomata of hydroponic bowl lotus is bigger and its amount is larger than that of soil-culture bowl lotus. At the same time, the ratio of the palisade tissue thickness to spongy tissue thickness is small,and its leaf tissue structure is loose. The root vigor of hydroponic bowl lotus reached its summit earlier, then began to drop. Whereas, the root activity of soil-culture lotus sustained increasing, with vigorous growth. [Conclusion] Therefore, it indicated that hydroponic bowl lotus can adapt to the aquatic-culture environment well and quickly, meanwhile, it also enters into its aging period quickly and its growth cycle gets shorter.
基金Supported by National Natural Science Foundation of China(31101131)National Key Technology Research and Development Program(2011BAD16B03)+1 种基金Agricultural Science Independent Innovation Foundation of Jiangsu Province[CX(12)1003]Key Technology Research and Development Program of Jiangsu Province(BE2012309)~~
文摘Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.
基金Supported by Tianjin University of Science & Technology LaboratoryOpen Fund(1014A109)National 863 Science Plan(2010AA101301)National Natural Science Foundation(20903071)~~
文摘[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.