Harmful cyanobacterial blooms, especially Microcystis blooms, occur worldwide and draw widespread attention. The dynamics of microcystin-producing Microcystis and competition between microcystin-producing Microcystis ...Harmful cyanobacterial blooms, especially Microcystis blooms, occur worldwide and draw widespread attention. The dynamics of microcystin-producing Microcystis and competition between microcystin-producing Microcystis and non-microcystin-producing Microcystis are key to predicting and treating Microcystis blooms. Multiplex qPCR is a useful tool to assess such issues. In this study, we developed multiplex qPCR methods with newly-designed probes and primers for the microcystin-synthesis related genes mcyA and mcyE. We used seven toxic Microcystis strains and four non-toxic Microcystis strains to compare the differences in the ratios of toxic and non-toxic Microcystis in mixed cultures, which were calculated using abundances of the genes mcyA, mcyB, mcyD, mcy E and phycocyanin( PC). We also compared traditional cell counting and multiplex qPCR. Hierarchical clustering and principal component analysis indicated that mcyD was the most suitable mcy gene for quantification in laboratory experiments. mcyB abundances were always higher; we suggest that the amount of toxic Microcystis measured using mcyB might overestimate the actual percentages.展开更多
The roles of androgens on cardiovascular physiology and pathophysiology are controversial as both beneficial and detrimental effects have been reported. Although the reasons for this discrepancy are unclear, multiple ...The roles of androgens on cardiovascular physiology and pathophysiology are controversial as both beneficial and detrimental effects have been reported. Although the reasons for this discrepancy are unclear, multiple factors such as genetic and epigenetic variation, sex-specificity, hormone interactions, drug preparation and route of administration may contribute. Recently, growing evidence suggests that androgens exhibit beneficial effects on cardiovascular function though the mechanism remains to be elucidated. Endothelial cells (ECs) which line the interior surface of blood vessels are distributed throughout the circulatory system, and play a crucial role in cardiovascular function. Endothelial progenitor cells (EPCs) are considered an indispensable element for the reconstitution and maintenance of an intact endothelial layer. Endothelial dysfunction is regarded as an initiating step in development of atherosclerosis and cardiovascular diseases. The modulation of endothelial functions by androgens through either genornic or nongenomic signal pathways is one possible mechanism by which androgens act on the cardiovascular system. Obtaining insight into the mechanisms by which androgens affect EC and EPC functions will allow us to determine whether androgens possess beneficial effects on the cardiovascular system. This in turn may be critical in the prevention and therapy of cardiovascular diseases. This article seeks to review recent progress in androgen regulation of endothelial function, the sex-specificity of androgen actions, and its clinical applications in the cardiovascular system.展开更多
This paper mainly design B/S system structure and small information management system that often appear some problems of college student information management, combines the actual situation of students and student in...This paper mainly design B/S system structure and small information management system that often appear some problems of college student information management, combines the actual situation of students and student instructor management work. In the process of the development of the whole system, the main use of Microsoft.NET technology as a whole system of compiler environment, SQL Server 2000 is used in the design of backstage supporter' s database, were developed and designed by Visual Studi02005 tool, in exchange for page dynamically selected by the ASP.NET database and system interface between Webpage generation. This system has high reliability, high security, low input, so that the efficiency of information management students to improve.展开更多
The species of Epithemia ocellata (Ehr.) Kuetz. from Northeastern China were observed by scanning electron microscopy (SEM). The studies on silica valve formation in different stages were made. At the early stage,...The species of Epithemia ocellata (Ehr.) Kuetz. from Northeastern China were observed by scanning electron microscopy (SEM). The studies on silica valve formation in different stages were made. At the early stage, a Y-shaped raphe sternum was found. Areolae were made from virgae and vimines. In immature valves, the canal formation before the raphe fissure is visible. The raphe system, virgae and vimines (areolae) might be the early siliceous elements of the valve during morphogenesis. The formations of virgae and vimines are similar to that of Gephyria media W. Arnott. Rows of silica papillae formed gradually on the face of the valve. After the areolae were covered with silica and form rows of papillae, the outer valve surface could become mature. Details of the internal costae were observed in mature valves.展开更多
The Chinese government adopted six ecological restoration programs to improve its natural environments. Although these programs have proven successful in improving local environments, some studies have questioned thei...The Chinese government adopted six ecological restoration programs to improve its natural environments. Although these programs have proven successful in improving local environments, some studies have questioned their performance when regions suffer from drought. Whether we should consider the effects of drought on vegetation change in assessments of the benefits of ecological restoration programs is unclear. Therefore, taking the Grain for Green Program(GGP) region as a study area, we estimated vegetation growth in the region from 2000–2010 to clarify the trends in vegetation and their driving forces. Results showed that: 1) vegetation growth increased in the GGP region during 2000–2010, with 59.4% of the area showing an increase in the Normalized Difference Vegetation Index(NDVI). This confirmed the benefits of the ecological restoration program. 2) Drought can affect the vegetation change trend, but human activity plays a significant role in altering vegetation growth, and the slight downward trend in the NDVI was not consistent with the severity of the drought. Positive human activity led to increased NDVI in 89.13% of areas. Of these, 22.52% suffered drought, but positive human activity offset the damage in part. 3) Results of this research suggest that appropriate human activity can maximize the benefits of ecological restoration programs and minimize the effects of extreme weather. We therefore recommend incorporating eco-risk assessment and scientific management mechanisms in the design and management of ecosystem restoration programs.展开更多
With the development of the theory of college students internship, mternship teaching this new mode ot teaching practice are paid much attention, this paper on teaching students internships supports the management of ...With the development of the theory of college students internship, mternship teaching this new mode ot teaching practice are paid much attention, this paper on teaching students internships supports the management of some rather special Teachers College and conducts a comprehensive in-depth investigation, and summarizes student internships management features and focus during the teaching on the basis of further exploration reasonable and effective teaching students internships branch management.展开更多
This paper analyzes the actual situation of the electricity management about student apartments, and design intelligent energy student housing management system based on CAN bus. The system uses the field level, the u...This paper analyzes the actual situation of the electricity management about student apartments, and design intelligent energy student housing management system based on CAN bus. The system uses the field level, the underlying management level and upper management level three management system. Field level with a dedicated energy metering chip AD7755 and STM32F103 microcontroller with A/D conversion function as the core, to achieve real-time power measurement; via CAN bus timing or random read live energy data for monitoring electricity consumption of the apartment, investigate abnormal electricity, thus effectively limiting the students to use electrical power to achieve the modernization and automation of power management solutions student apartments.展开更多
This paper uses oriented process method to analysis and design the system, using SQL Server 2000 database as the backend database support platform system, using three layer B/S architecture to support, the system prov...This paper uses oriented process method to analysis and design the system, using SQL Server 2000 database as the backend database support platform system, using three layer B/S architecture to support, the system provides a good interface and function feature, data have high safety performance, high reliability and low cost, the system can improve the work efficiency of college personnel, improve the efficiency of management, and has a certain practical significance.展开更多
Falling film generation process in lithium bromide absorption refrigeration generation system is researched in thispaper. To describe the coupled heat and mass transfer of laminar falling film in vertical generation t...Falling film generation process in lithium bromide absorption refrigeration generation system is researched in thispaper. To describe the coupled heat and mass transfer of laminar falling film in vertical generation tube, a mathematicalmodel is developed, in which the effect of mass transfer on heat transfer is carefully evaluated. Moreover,an equation related Re number with solution volume flow was also obtained in given conditions. We carried outthe experiments with the mass fraction of the inlet solution LiBr being 49.5% and obtained an empirical correlationof heat transfer with the film Re number and heat flux in different volume flow. Specifically, when 5kW/m2<qw<25kW/m2 and Re<500 the heat transfer correlation is given as: h=14009.87qw0.0764Re-0.5391.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31370418,41561144008)the Jiangxi Water Science and Technology Fund(No.KT201602)the State Key Laboratory of Freshwater Ecology and Biotechnology(No.2016FBZ07)
文摘Harmful cyanobacterial blooms, especially Microcystis blooms, occur worldwide and draw widespread attention. The dynamics of microcystin-producing Microcystis and competition between microcystin-producing Microcystis and non-microcystin-producing Microcystis are key to predicting and treating Microcystis blooms. Multiplex qPCR is a useful tool to assess such issues. In this study, we developed multiplex qPCR methods with newly-designed probes and primers for the microcystin-synthesis related genes mcyA and mcyE. We used seven toxic Microcystis strains and four non-toxic Microcystis strains to compare the differences in the ratios of toxic and non-toxic Microcystis in mixed cultures, which were calculated using abundances of the genes mcyA, mcyB, mcyD, mcy E and phycocyanin( PC). We also compared traditional cell counting and multiplex qPCR. Hierarchical clustering and principal component analysis indicated that mcyD was the most suitable mcy gene for quantification in laboratory experiments. mcyB abundances were always higher; we suggest that the amount of toxic Microcystis measured using mcyB might overestimate the actual percentages.
基金The researches described in this article were partially supported by grants from the National Natural Science Foundation of China (No. 81570271 and 81400357) and NIH (UL1 RR024996). We are very grateful to John R Lee (Assistant Professor of Medicine, Weill Comell Medical College, New York), and Jeff J Zhu (Research Manager, Weill Comell Medical College, New York) for critical review of the article. The authors have nothing to disclosure.
文摘The roles of androgens on cardiovascular physiology and pathophysiology are controversial as both beneficial and detrimental effects have been reported. Although the reasons for this discrepancy are unclear, multiple factors such as genetic and epigenetic variation, sex-specificity, hormone interactions, drug preparation and route of administration may contribute. Recently, growing evidence suggests that androgens exhibit beneficial effects on cardiovascular function though the mechanism remains to be elucidated. Endothelial cells (ECs) which line the interior surface of blood vessels are distributed throughout the circulatory system, and play a crucial role in cardiovascular function. Endothelial progenitor cells (EPCs) are considered an indispensable element for the reconstitution and maintenance of an intact endothelial layer. Endothelial dysfunction is regarded as an initiating step in development of atherosclerosis and cardiovascular diseases. The modulation of endothelial functions by androgens through either genornic or nongenomic signal pathways is one possible mechanism by which androgens act on the cardiovascular system. Obtaining insight into the mechanisms by which androgens affect EC and EPC functions will allow us to determine whether androgens possess beneficial effects on the cardiovascular system. This in turn may be critical in the prevention and therapy of cardiovascular diseases. This article seeks to review recent progress in androgen regulation of endothelial function, the sex-specificity of androgen actions, and its clinical applications in the cardiovascular system.
文摘This paper mainly design B/S system structure and small information management system that often appear some problems of college student information management, combines the actual situation of students and student instructor management work. In the process of the development of the whole system, the main use of Microsoft.NET technology as a whole system of compiler environment, SQL Server 2000 is used in the design of backstage supporter' s database, were developed and designed by Visual Studi02005 tool, in exchange for page dynamically selected by the ASP.NET database and system interface between Webpage generation. This system has high reliability, high security, low input, so that the efficiency of information management students to improve.
基金National Natural Science Foundation of China (30270111)Natural Science Foundation of Heilongjiang Province (C 2303)+1 种基金Project of Innovation Capacity of Teachers in University (1055G018)Post-doctor Start-up Fund of the Heilongjiang Provincial Government.
文摘The species of Epithemia ocellata (Ehr.) Kuetz. from Northeastern China were observed by scanning electron microscopy (SEM). The studies on silica valve formation in different stages were made. At the early stage, a Y-shaped raphe sternum was found. Areolae were made from virgae and vimines. In immature valves, the canal formation before the raphe fissure is visible. The raphe system, virgae and vimines (areolae) might be the early siliceous elements of the valve during morphogenesis. The formations of virgae and vimines are similar to that of Gephyria media W. Arnott. Rows of silica papillae formed gradually on the face of the valve. After the areolae were covered with silica and form rows of papillae, the outer valve surface could become mature. Details of the internal costae were observed in mature valves.
基金Under the auspices of the National Key R&D Program of China(No.2017YFC0504701)Science and Technology Service Network Initiative Project of Chinese Academy of Sciences(No.KFJ-STS-ZDTP-036)+1 种基金Fundamental Research Funds for the Central Universities(No.GK201703053)China Postdoctoral Science Foundation(No.2017M623114)
文摘The Chinese government adopted six ecological restoration programs to improve its natural environments. Although these programs have proven successful in improving local environments, some studies have questioned their performance when regions suffer from drought. Whether we should consider the effects of drought on vegetation change in assessments of the benefits of ecological restoration programs is unclear. Therefore, taking the Grain for Green Program(GGP) region as a study area, we estimated vegetation growth in the region from 2000–2010 to clarify the trends in vegetation and their driving forces. Results showed that: 1) vegetation growth increased in the GGP region during 2000–2010, with 59.4% of the area showing an increase in the Normalized Difference Vegetation Index(NDVI). This confirmed the benefits of the ecological restoration program. 2) Drought can affect the vegetation change trend, but human activity plays a significant role in altering vegetation growth, and the slight downward trend in the NDVI was not consistent with the severity of the drought. Positive human activity led to increased NDVI in 89.13% of areas. Of these, 22.52% suffered drought, but positive human activity offset the damage in part. 3) Results of this research suggest that appropriate human activity can maximize the benefits of ecological restoration programs and minimize the effects of extreme weather. We therefore recommend incorporating eco-risk assessment and scientific management mechanisms in the design and management of ecosystem restoration programs.
文摘With the development of the theory of college students internship, mternship teaching this new mode ot teaching practice are paid much attention, this paper on teaching students internships supports the management of some rather special Teachers College and conducts a comprehensive in-depth investigation, and summarizes student internships management features and focus during the teaching on the basis of further exploration reasonable and effective teaching students internships branch management.
文摘This paper analyzes the actual situation of the electricity management about student apartments, and design intelligent energy student housing management system based on CAN bus. The system uses the field level, the underlying management level and upper management level three management system. Field level with a dedicated energy metering chip AD7755 and STM32F103 microcontroller with A/D conversion function as the core, to achieve real-time power measurement; via CAN bus timing or random read live energy data for monitoring electricity consumption of the apartment, investigate abnormal electricity, thus effectively limiting the students to use electrical power to achieve the modernization and automation of power management solutions student apartments.
文摘This paper uses oriented process method to analysis and design the system, using SQL Server 2000 database as the backend database support platform system, using three layer B/S architecture to support, the system provides a good interface and function feature, data have high safety performance, high reliability and low cost, the system can improve the work efficiency of college personnel, improve the efficiency of management, and has a certain practical significance.
文摘Falling film generation process in lithium bromide absorption refrigeration generation system is researched in thispaper. To describe the coupled heat and mass transfer of laminar falling film in vertical generation tube, a mathematicalmodel is developed, in which the effect of mass transfer on heat transfer is carefully evaluated. Moreover,an equation related Re number with solution volume flow was also obtained in given conditions. We carried outthe experiments with the mass fraction of the inlet solution LiBr being 49.5% and obtained an empirical correlationof heat transfer with the film Re number and heat flux in different volume flow. Specifically, when 5kW/m2<qw<25kW/m2 and Re<500 the heat transfer correlation is given as: h=14009.87qw0.0764Re-0.5391.