AIM： To study whether severe acute respiratory syndrome coronavirus （SARS-CoV） could be excreted from digestive system.METHODS： Cell culture and semi-nested RT-PCR were used to detect SARS-CoV and its RNA from 21 ...AIM： To study whether severe acute respiratory syndrome coronavirus （SARS-CoV） could be excreted from digestive system.METHODS： Cell culture and semi-nested RT-PCR were used to detect SARS-CoV and its RNA from 21 stool and urine samples, and a kind of electropositive filter media particles was used to concentrate the virus in 10 sewage samples from two hospitals receiving SAILS patients in Beijing in China. RESULTS： It was demonstrated that there was no live SARS-CoV in all samples collected, but the RNA of SARS-CoV could be detected in seven stool samples from SARS patients with any one of the symptoms of fever, malaise, cough, or dyspnea, in 10 sewage samples before disinfection and 3 samples after disinfection from the two hospitals. The RNA could not be detected in urine and stool samples from patients recovered from SARS.CONCLUSION： Nucleic acid of SARS-CoV can be excreted through the stool of patients into sewage system, and the possibility of SARS-CoV transmitting through digestive system cannot be excluded.展开更多
基金Supported by the National High Technology Research and Development Program of China, 863 Program, No. 2004AA649100 and the National Natural Science Foundation of China, No. 30471436
文摘AIM： To study whether severe acute respiratory syndrome coronavirus （SARS-CoV） could be excreted from digestive system.METHODS： Cell culture and semi-nested RT-PCR were used to detect SARS-CoV and its RNA from 21 stool and urine samples, and a kind of electropositive filter media particles was used to concentrate the virus in 10 sewage samples from two hospitals receiving SAILS patients in Beijing in China. RESULTS： It was demonstrated that there was no live SARS-CoV in all samples collected, but the RNA of SARS-CoV could be detected in seven stool samples from SARS patients with any one of the symptoms of fever, malaise, cough, or dyspnea, in 10 sewage samples before disinfection and 3 samples after disinfection from the two hospitals. The RNA could not be detected in urine and stool samples from patients recovered from SARS.CONCLUSION： Nucleic acid of SARS-CoV can be excreted through the stool of patients into sewage system, and the possibility of SARS-CoV transmitting through digestive system cannot be excluded.
