目的本研究拟证实利用高渗盐水进行液体复苏可保护缺血-再灌注过程中的肠道黏膜屏障功能,并减轻该过程中机体系统性炎症反应综合征(SIRS),从而为临床大出血、严重感染、大面积烧伤后肠功能障碍患者液体复苏方案的选择提供新的策略方法...目的本研究拟证实利用高渗盐水进行液体复苏可保护缺血-再灌注过程中的肠道黏膜屏障功能,并减轻该过程中机体系统性炎症反应综合征(SIRS),从而为临床大出血、严重感染、大面积烧伤后肠功能障碍患者液体复苏方案的选择提供新的策略方法和理论依据.方法①选用清洁级雄性Sprague-Dawley(SD)大鼠,体重250g-300g,通过肠系膜上动脉夹闭法建立大鼠小肠缺血-再灌注损伤模型。②选用清洁级雄性Sprague-Dawley(SD)大鼠,随机分为假手术组(Sham组),缺血-再灌注损伤组(IR组)和高渗盐水复苏组(HS组)。IR组夹闭肠系膜上动脉1h后开放并经尾静脉输入0.9%氯化钠溶液;HS组夹闭肠系膜上动脉1h后开放并经尾静脉输入7.5%氯化钠溶液;Sham组操作方法同IR组,但不进行肠系膜上动脉夹闭。再灌注6h后取标本。③免疫组化检测小肠粘膜内淋巴细胞(intraepithelial lymphocyte,IEL)和固有层淋巴细胞(lamina propria lymphocytes,LPL)中T细胞亚群改变;流式细胞术检测大鼠外周血T细胞亚群及NK细胞。结果①肠黏膜上皮细胞层中的上皮内淋巴细胞(IEL)和位于疏松结缔组织中的固有层淋巴细胞(LPL)中CD4+T细胞数量和CD8+细胞数量,IR组和HS组显著高于Sham组,HS组显著高于IR组。②外周血T细胞亚群及NK分析:HS组CD3+CD8+T细胞显著低于IR组和Sham组(P<0.05),HS组NK细胞比例显著低于IR组和NS组(P<0.05),余未见显著性差异。结论利用高渗盐水复苏缺血小肠,可有效保护小肠免疫屏障,抑制全身炎性反应综合征。展开更多
Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escheri...Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50=11.51 mg/kg), but CT DNA didn't. The peak level of TNF-αwas lower in EC DNA group than in LPS group (P＜0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of serum TNF-αand IL-6 levels in rats. EC DNA has the effect equal to LPS in inducing SIRS by triggering cytokines cascade.展开更多
文摘目的本研究拟证实利用高渗盐水进行液体复苏可保护缺血-再灌注过程中的肠道黏膜屏障功能,并减轻该过程中机体系统性炎症反应综合征(SIRS),从而为临床大出血、严重感染、大面积烧伤后肠功能障碍患者液体复苏方案的选择提供新的策略方法和理论依据.方法①选用清洁级雄性Sprague-Dawley(SD)大鼠,体重250g-300g,通过肠系膜上动脉夹闭法建立大鼠小肠缺血-再灌注损伤模型。②选用清洁级雄性Sprague-Dawley(SD)大鼠,随机分为假手术组(Sham组),缺血-再灌注损伤组(IR组)和高渗盐水复苏组(HS组)。IR组夹闭肠系膜上动脉1h后开放并经尾静脉输入0.9%氯化钠溶液;HS组夹闭肠系膜上动脉1h后开放并经尾静脉输入7.5%氯化钠溶液;Sham组操作方法同IR组,但不进行肠系膜上动脉夹闭。再灌注6h后取标本。③免疫组化检测小肠粘膜内淋巴细胞(intraepithelial lymphocyte,IEL)和固有层淋巴细胞(lamina propria lymphocytes,LPL)中T细胞亚群改变;流式细胞术检测大鼠外周血T细胞亚群及NK细胞。结果①肠黏膜上皮细胞层中的上皮内淋巴细胞(IEL)和位于疏松结缔组织中的固有层淋巴细胞(LPL)中CD4+T细胞数量和CD8+细胞数量,IR组和HS组显著高于Sham组,HS组显著高于IR组。②外周血T细胞亚群及NK分析:HS组CD3+CD8+T细胞显著低于IR组和Sham组(P<0.05),HS组NK细胞比例显著低于IR组和NS组(P<0.05),余未见显著性差异。结论利用高渗盐水复苏缺血小肠,可有效保护小肠免疫屏障,抑制全身炎性反应综合征。
基金National Natural Science Foundation of China (No. 30070299)
文摘Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50=11.51 mg/kg), but CT DNA didn't. The peak level of TNF-αwas lower in EC DNA group than in LPS group (P＜0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of serum TNF-αand IL-6 levels in rats. EC DNA has the effect equal to LPS in inducing SIRS by triggering cytokines cascade.
