期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
基于酶学-细胞水平的IDO1抑制剂筛选模型建立与优化
1
作者 来芳芳 薛妮娜 +4 位作者 季鸣 杜婷婷 李凌 盛莉 陈晓光 《药学学报》 CAS CSCD 北大核心 2019年第10期1868-1874,共7页
本研究以肿瘤免疫治疗相关蛋白吲哚胺2,3-双加氧酶1 (indoleamine 2,3-dioxygenase 1, IDO1)为靶点,提出并设计构建了基于酶学-细胞水平的IDO1抑制剂筛选模型并进行了体内验证。首先采用基因工程手段表达纯化重组人IDO1蛋白并建立简单... 本研究以肿瘤免疫治疗相关蛋白吲哚胺2,3-双加氧酶1 (indoleamine 2,3-dioxygenase 1, IDO1)为靶点,提出并设计构建了基于酶学-细胞水平的IDO1抑制剂筛选模型并进行了体内验证。首先采用基因工程手段表达纯化重组人IDO1蛋白并建立简单高效的IDO酶学筛选体系;其次通过干扰素γ(interferon-γ, IFNγ)刺激A172细胞或者构建高表达人IDO1蛋白的质粒瞬时转染HEK293细胞构建特异性的IDO1细胞水平筛选体系;最后在C57小鼠通过LC/MS/MS方法检测小鼠血浆中犬尿氨酸和色氨酸的含量和比值,从体内进一步验证筛选得到的化合物对IDO酶的抑制作用。本研究中建立和优化的酶学、细胞水平的筛选模型再结合体内的验证方法能高效、通量、特异性筛选到真正体内有效的IDO1抑制剂,为新药的快速研发奠定了良好的基础。动物实验均严格遵循中国医学科学院药物研究所动物实验中心标准操作规程,尽量减少实验对动物造成的伤害。 展开更多
关键词 高通量 系统筛选模型 吲哚胺2 3-双加氧酶1 抑制剂 肿瘤免疫
原文传递
Framework Design of Eco-Technology Evaluation Platform and Integration System
2
作者 肖玉 谢高地 甄霖 《Journal of Resources and Ecology》 CSCD 2017年第4期325-331,共7页
Global ecological degradation is a matter of enormous concern. In the early 20 st century, the United States, Europe and China began to apply eco-technology to ecosystem management and restoration in order to slow dow... Global ecological degradation is a matter of enormous concern. In the early 20 st century, the United States, Europe and China began to apply eco-technology to ecosystem management and restoration in order to slow down or stop ecological degradation. To date, there has been neither a systematic summary and scientific evaluation, nor is there a unified platform to describe ecological degradation problems in different areas and existing eco-technologies. These shortcomings have hindered the popularization and application of technologies. This study intends to build an eco-technology evaluation platform and integration system that brings together heterogeneous data from multiple sources. The key technology of the eco-technology evaluation platform and integration system is information integration technology. We will establish a metadata engine based on metadata storage to achieve access to and integration of metadata and heterogeneous data sources. The information integration mode based on a metamodel addresses information heterogeneity at four levels: system, syntax, structure and semantics. We develop the framework for an eco-technology evaluation platform and integration system to integrate ecotechnology databases, eco-technology evaluation model databases, eco-technology evaluation parameter databases and spatial databases of ecological degradation and eco-technology with metadata and metamodel integration mode. This system can support functions for the query and display of global and typical ecological degradation and the query, display, evaluation and prioritization of eco-technologies, which can realize the visualization of global and Chinese ecological degradation and eco-technology evaluation and prioritization. This system will help government decision makers and relevant departments to understand ecological degradation and the effects of ecotechnology implementation. 展开更多
关键词 eco-technology evaluation eco-technology prioritization integrated system METADATA METAMODEL
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部