[Objective] The aim was to study rooting environment of strawberry "Bechi- ka" seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence f...[Objective] The aim was to study rooting environment of strawberry "Bechi- ka" seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that: 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% (perlite:vermiculite=l:l). Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.展开更多
Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds a...Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients (nitrogen, phosphorus, silicon, and sulfur), light intensity and light path, on O. aurita cell growth and biochemical composition (protein, carbohydrate, β-1,3-glucan, lipids, and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of 15-1,3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.展开更多
The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4LDM (quadrupled concentration of the nutrient salt) was the optimal con...The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL^-1 or higher than 15 gL^-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34 gL^-1 was obtained in the presence of 150 mgL^-1 Na2SiO3· 9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150mgL^-1; however, higher silicate concentrations resulted in a steady decrease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110mgL^-1) were observed at the salinity of 36 and 29, respectively. Key words polyunsaturated fatty acid; eicosapentaenoic acid; microalga; Nitzschia laevis; heterotrophy展开更多
A study was carried out to investigate changes in the soil plant-available P,the P nutrition and the growth of Pinus radiata seedlings grown in association with understory,broom(Cytisus scoparius L.) or ryegrass(Lo...A study was carried out to investigate changes in the soil plant-available P,the P nutrition and the growth of Pinus radiata seedlings grown in association with understory,broom(Cytisus scoparius L.) or ryegrass(Lolium multiflorum) on Orthic Allophanic Soil,following the application of three rates of triple superphosphate(TSP)(0,50,and 100 mg·kg^-1P) under a glasshouse condition.The application of P fertilizer enhanced P availability in the rhizospheric of radiata seedlings and the bulk soils in a P-deficient site.P availability in the rhizospheric soils of ryegrass and broom,grown in association with radiata,were also increased by the presence of radiata roots.P concentrations in new shoot needles,old shoot needles,stem and roots of radiata pine increased with increase rates of TSP application,but the effects of ryegrass and broom on P nutrition of radiata seedlings depended on the soil P status.In the absence of P fertilizer addition(control treatment),P concentrations in new shoot needles,old shoot needles,stem,and roots of radiata grown in association with broom were higher than those with ryegrass,whereas,when P fertilizer was added(50 and 100 mg·kg^-1) the P concentration was lower.This is probably related to the growth of broom that may have removed much of the plant-available P in the soil as indicated by the consistently lower Bray-2 P concentration in the rhizosphere soil of radiata in association with broom than that in the rhizosphere soil of radiata in association with grass at the two high P rates.Furthermore,in the high P fertile soil(application rate of 100 mg·kg^-1),the dry matter yield of radiata was lower when it was grown with broom than with ryegrass.This result suggests that in moderate to high P fertile soils,P.radiata seedlings grow better with ryegrass than with broom,because broom grows vigorously in high P fertile soil and competes with P.radiata for P and perhaps for other nutrients as well.展开更多
The effects of different phosphorus(P) concentrations(0.36, 3.6, and 36 μmol/L corresponding to low-, middle-, and high-P concentration groups, respectively) and nitrogen(N)/P ratios on the growth and photosynthetic ...The effects of different phosphorus(P) concentrations(0.36, 3.6, and 36 μmol/L corresponding to low-, middle-, and high-P concentration groups, respectively) and nitrogen(N)/P ratios on the growth and photosynthetic characteristics of S keletonema costatum and Prorocentrum donghaiense were studied. For both species, the high-P(HP) concentration group showed the greatest algal density and highest specifi c growth rate. Changes in the maximum effi ciency of photosystem Ⅱ(F _v/F_m) were monitored under the various P and N/P conditions. The largest decrease in F _v/F_m was in the low-P(LP) group in S. costatum and in the HP group in P. donghaiense. There were high rapid light curves and photochemical quantum yields(Φ _(PSⅡ)) for S. costatum in the HP group, while the actual photosynthetic capacity was higher in P. donghaiense than in S. costatum in the MP group. Under eutrophic but relatively P-restricted conditions, P. donghaiense had higher photosynthetic activity and potential, which could cause this dinofl agellate to increasingly dominate the phytoplankton community in these conditions. Under the same P concentration and N/P ratio, P. donghaiense had a larger relative maximum rate of electron transport and higher Φ _(PSⅡ) values than those of S. costatum. These differences between P. donghaiense and S. costatum may explain the interaction and succession patterns of these two species in the Changjiang(Yangtze) River estuary from a photosynthesis perspective.展开更多
The Cape fynbos is characterised by highly leached, sandy, acidic soils with very low nutrient concentrations. Plant-available P levels range from 0.4 μg P g-1 to 3.7 μg P g-I soil, and 1-2 mg N gl soil. Despite the...The Cape fynbos is characterised by highly leached, sandy, acidic soils with very low nutrient concentrations. Plant-available P levels range from 0.4 μg P g-1 to 3.7 μg P g-I soil, and 1-2 mg N gl soil. Despite these low nutrient concentrations, the fynbos is home to 9,030 vascular plant species with 68.7% endemicity. How native plant species survive such low levels of available P is intriguing, and indeed the subject of this review. In the fynbos soils, P is easily precipitated with cations such as Fe and Al, forming AI-P and Fe-P in acidic soils, or Ca-P in neutral-to-alkaline soils. The mechanisms for promoting P availability and enhancing P nutrition include the development of mycorrhizal symbiosis (with 80%-90% of higher plants, e.g., Cyclopia, Aspalathus, Psoralea and Leucadendron etc.) which exhibits 3-5 times much greater P acquisition than non-mycorrhizal roots. Formation of cluster roots by the Leguminosae (Fabaceae) and their exudation of Kreb cycle intermediates (organic acids) for solubilizing P, secretion of root exudate compounds (organic acids, phenolics, amino acids, etc.) that mobilize P. The synthesis and release of acid and alkaline phosphatase enzyme that catalyze the cleavage of mineral P from organic phosphate esters in acidic and alkaline soils, and the development of deep tap roots as well as massive secondary roots within the uppermost 15 cm of soil for capturing water and nutrients. Some fynbos legumes employ all these adaptive mechanisms for enhancing P nutrition and plant growth. Aspalathus and Cyclopia species typically form mycorrhizal and rhizobial symbiosis for improving P and N nutrition, produce cluster roots and acid phosphatases for increasing P supply, and release root exudates that enhance P solubilisation and uptake.展开更多
Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate (SGR) and chlorophyll fluores...Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate (SGR) and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50, and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence (F_v/F_m) were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO_4-P. The photosynthetic capacity of photosystem Ⅱ (PSⅡ) was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO_3-N concentrations were greater than 1.0 mmol/L and when PO4-P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera, these data contribute important information to ensure optimal results.展开更多
By using nutritional diagnosis of citrus leaves and determining soil micronutrients,the relationship between soil micronutrients and citrus growth in southern China has been studied.Studies showed that there was a sig...By using nutritional diagnosis of citrus leaves and determining soil micronutrients,the relationship between soil micronutrients and citrus growth in southern China has been studied.Studies showed that there was a significant positive correlation between available micronutrients (such as Zn,Mo,Cu)in the soil and the corresponding nutrients in citrus leaves.Thus,one can roughly learn of the sufficiency or deficiency of certain nutrients in soils by analyzing citrus leaves.Rational spray of Zn B or Mo fertilizer not only improved citrus yields but also increased the total sugar of Satsuma mandarin and of Xinhui orange by 2.9 and 17.2% respectively compared with the control.Spraying Mo fertilizer increased the vitamin C content of Satsuma mandarin juice by 4.7%-8.4%,maturated fruits 7-10 days earlier and gave the peel a brighter color.The ultramicroscopic characteristics of Zn-deficient citrus leaves were investigated under an electron microscope.Results showed that the Zn-deficient leaf cell was characterized mainly by poor cytoplasm,endoplasmic reticula and ribosomes and by big starch grains in the chloroplast.As a result of spraying Zn fertilizer the structure of the cell returned to normal,the cytoplasm became rich and the amount of chloroplast increased.There also appeared a great deal of multiform endoplasmic reticula,thus promoting the photosynthesis of Zn-deficient plants.This provides a cytologico-theoretical basis for fertilization of high-yielding citrus trees.展开更多
Vector analysis technique and ecological sequential comparison methods were adopted to study tree growth response to the micronutrients, B, Mo, Cu, Zn, Fe, and Mn, in soils derived from various parent materials in the...Vector analysis technique and ecological sequential comparison methods were adopted to study tree growth response to the micronutrients, B, Mo, Cu, Zn, Fe, and Mn, in soils derived from various parent materials in the forest area of Taihu Lake region in southeast China. The results showed that the dry weight of individual current-year needle of Chinese fir (Cunninghamia lanceolata) grown on the soi1 derived from granite parent rock was increased by 8% and 13% in comparison with that grown on the soils derived from sandstone and ash-tuff parent rock, respectively. And such dry weight of loblolly pine (Pinus taeda) grown on the soil derived from sandstone parent rock was increased by 21% in comparison with that on the soil derived from ash-tuff parent rock. One of the reasons for those results was that micronutrients content in the soil derived from ash-tuff parent rock were not sufficient to meet the requirement of the growth of Chinese fir and loblolly pine, i.e., micronutrients in soil were deficient and/or induced deficient. The amounts of Cu, Zn, Fe, and Mn uptake by Chinese fir and loblolly pine were in agreement with the contents of available micronutrients in soil respectively, except for B and Mo. Meanwhile, there might exist an "antagonism" between the uptake of B versus Mo by trees, although more studies are needed to confirm it. Regression analysis indicated that amount of a soil available micronutrient was correlated to the type of parent material and its total amount in the forest floor, except for B. The F test identified that the correlation of each equation reached the significant level to different extents, respectively. The t test confirmed that amount of available forms was mainly depended on the type of parent material for Mo, Cu, Zn and Mn but on the forest floor for Fe. There was a feedback effect of forest stand on the amount of soil available micronutrients. The ability of accumulating available micronutrients in soil was better by the sawtooth oak (Quercus acutissima) stand than by the Chinese fir stand (except for B). The ability of accumulating available Zn, Fe, Mn and Mo in soil was better by the Chinese fir stand than by the loblolly pine stand, while as for available B and Cu, by the latter was better than by the former. When discussing the effect of forest stand on the amount of soil available micronutrients, not only the amount of micronutrient in the forest floor and the parent materials but also the amount of micronutrient taken up by current-year needles have to be considered.展开更多
文摘[Objective] The aim was to study rooting environment of strawberry "Bechi- ka" seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that: 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% (perlite:vermiculite=l:l). Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2009AA06440,2013AA065805)the National Basic Research Program of China(973 Program)(No.2011CB2009001)the National Natural Science Foundation of China(No.31170337)
文摘Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients (nitrogen, phosphorus, silicon, and sulfur), light intensity and light path, on O. aurita cell growth and biochemical composition (protein, carbohydrate, β-1,3-glucan, lipids, and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of 15-1,3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.
文摘The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL^-1 or higher than 15 gL^-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34 gL^-1 was obtained in the presence of 150 mgL^-1 Na2SiO3· 9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150mgL^-1; however, higher silicate concentrations resulted in a steady decrease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110mgL^-1) were observed at the salinity of 36 and 29, respectively. Key words polyunsaturated fatty acid; eicosapentaenoic acid; microalga; Nitzschia laevis; heterotrophy
基金supported by Massey University and the Centre for Sustainable Forest Management at Forest Research Institute, New Zealand
文摘A study was carried out to investigate changes in the soil plant-available P,the P nutrition and the growth of Pinus radiata seedlings grown in association with understory,broom(Cytisus scoparius L.) or ryegrass(Lolium multiflorum) on Orthic Allophanic Soil,following the application of three rates of triple superphosphate(TSP)(0,50,and 100 mg·kg^-1P) under a glasshouse condition.The application of P fertilizer enhanced P availability in the rhizospheric of radiata seedlings and the bulk soils in a P-deficient site.P availability in the rhizospheric soils of ryegrass and broom,grown in association with radiata,were also increased by the presence of radiata roots.P concentrations in new shoot needles,old shoot needles,stem and roots of radiata pine increased with increase rates of TSP application,but the effects of ryegrass and broom on P nutrition of radiata seedlings depended on the soil P status.In the absence of P fertilizer addition(control treatment),P concentrations in new shoot needles,old shoot needles,stem,and roots of radiata grown in association with broom were higher than those with ryegrass,whereas,when P fertilizer was added(50 and 100 mg·kg^-1) the P concentration was lower.This is probably related to the growth of broom that may have removed much of the plant-available P in the soil as indicated by the consistently lower Bray-2 P concentration in the rhizosphere soil of radiata in association with broom than that in the rhizosphere soil of radiata in association with grass at the two high P rates.Furthermore,in the high P fertile soil(application rate of 100 mg·kg^-1),the dry matter yield of radiata was lower when it was grown with broom than with ryegrass.This result suggests that in moderate to high P fertile soils,P.radiata seedlings grow better with ryegrass than with broom,because broom grows vigorously in high P fertile soil and competes with P.radiata for P and perhaps for other nutrients as well.
基金Supported by the“Strategic Priority Research Program-Climate Change:Carbon Budget and Relevant Issues”of Chinese Academy of Sciences(No.XDA05030401)the National Basic Research Program of China(973 Program)(No.2014CB441504)+1 种基金the Key Program of National Natural Science Foundation of China(No.41230963)the NSFCShandong Province Joint Fund Project(No.U1406403)
文摘The effects of different phosphorus(P) concentrations(0.36, 3.6, and 36 μmol/L corresponding to low-, middle-, and high-P concentration groups, respectively) and nitrogen(N)/P ratios on the growth and photosynthetic characteristics of S keletonema costatum and Prorocentrum donghaiense were studied. For both species, the high-P(HP) concentration group showed the greatest algal density and highest specifi c growth rate. Changes in the maximum effi ciency of photosystem Ⅱ(F _v/F_m) were monitored under the various P and N/P conditions. The largest decrease in F _v/F_m was in the low-P(LP) group in S. costatum and in the HP group in P. donghaiense. There were high rapid light curves and photochemical quantum yields(Φ _(PSⅡ)) for S. costatum in the HP group, while the actual photosynthetic capacity was higher in P. donghaiense than in S. costatum in the MP group. Under eutrophic but relatively P-restricted conditions, P. donghaiense had higher photosynthetic activity and potential, which could cause this dinofl agellate to increasingly dominate the phytoplankton community in these conditions. Under the same P concentration and N/P ratio, P. donghaiense had a larger relative maximum rate of electron transport and higher Φ _(PSⅡ) values than those of S. costatum. These differences between P. donghaiense and S. costatum may explain the interaction and succession patterns of these two species in the Changjiang(Yangtze) River estuary from a photosynthesis perspective.
文摘The Cape fynbos is characterised by highly leached, sandy, acidic soils with very low nutrient concentrations. Plant-available P levels range from 0.4 μg P g-1 to 3.7 μg P g-I soil, and 1-2 mg N gl soil. Despite these low nutrient concentrations, the fynbos is home to 9,030 vascular plant species with 68.7% endemicity. How native plant species survive such low levels of available P is intriguing, and indeed the subject of this review. In the fynbos soils, P is easily precipitated with cations such as Fe and Al, forming AI-P and Fe-P in acidic soils, or Ca-P in neutral-to-alkaline soils. The mechanisms for promoting P availability and enhancing P nutrition include the development of mycorrhizal symbiosis (with 80%-90% of higher plants, e.g., Cyclopia, Aspalathus, Psoralea and Leucadendron etc.) which exhibits 3-5 times much greater P acquisition than non-mycorrhizal roots. Formation of cluster roots by the Leguminosae (Fabaceae) and their exudation of Kreb cycle intermediates (organic acids) for solubilizing P, secretion of root exudate compounds (organic acids, phenolics, amino acids, etc.) that mobilize P. The synthesis and release of acid and alkaline phosphatase enzyme that catalyze the cleavage of mineral P from organic phosphate esters in acidic and alkaline soils, and the development of deep tap roots as well as massive secondary roots within the uppermost 15 cm of soil for capturing water and nutrients. Some fynbos legumes employ all these adaptive mechanisms for enhancing P nutrition and plant growth. Aspalathus and Cyclopia species typically form mycorrhizal and rhizobial symbiosis for improving P and N nutrition, produce cluster roots and acid phosphatases for increasing P supply, and release root exudates that enhance P solubilisation and uptake.
基金Supported by the Technology Program of Basic Research of Qingdao(No.12-1-4-8-(2)-jch)
文摘Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate (SGR) and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50, and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence (F_v/F_m) were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO_4-P. The photosynthetic capacity of photosystem Ⅱ (PSⅡ) was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO_3-N concentrations were greater than 1.0 mmol/L and when PO4-P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera, these data contribute important information to ensure optimal results.
文摘By using nutritional diagnosis of citrus leaves and determining soil micronutrients,the relationship between soil micronutrients and citrus growth in southern China has been studied.Studies showed that there was a significant positive correlation between available micronutrients (such as Zn,Mo,Cu)in the soil and the corresponding nutrients in citrus leaves.Thus,one can roughly learn of the sufficiency or deficiency of certain nutrients in soils by analyzing citrus leaves.Rational spray of Zn B or Mo fertilizer not only improved citrus yields but also increased the total sugar of Satsuma mandarin and of Xinhui orange by 2.9 and 17.2% respectively compared with the control.Spraying Mo fertilizer increased the vitamin C content of Satsuma mandarin juice by 4.7%-8.4%,maturated fruits 7-10 days earlier and gave the peel a brighter color.The ultramicroscopic characteristics of Zn-deficient citrus leaves were investigated under an electron microscope.Results showed that the Zn-deficient leaf cell was characterized mainly by poor cytoplasm,endoplasmic reticula and ribosomes and by big starch grains in the chloroplast.As a result of spraying Zn fertilizer the structure of the cell returned to normal,the cytoplasm became rich and the amount of chloroplast increased.There also appeared a great deal of multiform endoplasmic reticula,thus promoting the photosynthesis of Zn-deficient plants.This provides a cytologico-theoretical basis for fertilization of high-yielding citrus trees.
基金Project supported by the National Natural Science Foundation of China (No. 39370563)by the NationalKey Basic Research Suppor
文摘Vector analysis technique and ecological sequential comparison methods were adopted to study tree growth response to the micronutrients, B, Mo, Cu, Zn, Fe, and Mn, in soils derived from various parent materials in the forest area of Taihu Lake region in southeast China. The results showed that the dry weight of individual current-year needle of Chinese fir (Cunninghamia lanceolata) grown on the soi1 derived from granite parent rock was increased by 8% and 13% in comparison with that grown on the soils derived from sandstone and ash-tuff parent rock, respectively. And such dry weight of loblolly pine (Pinus taeda) grown on the soil derived from sandstone parent rock was increased by 21% in comparison with that on the soil derived from ash-tuff parent rock. One of the reasons for those results was that micronutrients content in the soil derived from ash-tuff parent rock were not sufficient to meet the requirement of the growth of Chinese fir and loblolly pine, i.e., micronutrients in soil were deficient and/or induced deficient. The amounts of Cu, Zn, Fe, and Mn uptake by Chinese fir and loblolly pine were in agreement with the contents of available micronutrients in soil respectively, except for B and Mo. Meanwhile, there might exist an "antagonism" between the uptake of B versus Mo by trees, although more studies are needed to confirm it. Regression analysis indicated that amount of a soil available micronutrient was correlated to the type of parent material and its total amount in the forest floor, except for B. The F test identified that the correlation of each equation reached the significant level to different extents, respectively. The t test confirmed that amount of available forms was mainly depended on the type of parent material for Mo, Cu, Zn and Mn but on the forest floor for Fe. There was a feedback effect of forest stand on the amount of soil available micronutrients. The ability of accumulating available micronutrients in soil was better by the sawtooth oak (Quercus acutissima) stand than by the Chinese fir stand (except for B). The ability of accumulating available Zn, Fe, Mn and Mo in soil was better by the Chinese fir stand than by the loblolly pine stand, while as for available B and Cu, by the latter was better than by the former. When discussing the effect of forest stand on the amount of soil available micronutrients, not only the amount of micronutrient in the forest floor and the parent materials but also the amount of micronutrient taken up by current-year needles have to be considered.