Synthesis of Citrinin Artificial Antigen and Its Coupling Ratio Determination

[Objective] This study was to establish a method for the rapid and accurate detection of the coupling ratio of artificial antigen. [Method] Artificial synthetic antigen of citrinin （CIT） was first conjugated with vector protein bovine serum albumin （BSA） and then identified by SDS-PAGE electrophoresis,and its coupling ratio was measured by UV absorption and mass spectrometry. [Result] The identification result showed that artificial antigen of CIT was successfully coupled,and the coupling ratio was 8.4 by UV absorption while 6.0 by mass spectrometry. [Conclusion] The comparison experiment showed that mass spectrometry could rapidly identify the artificial antigen and accurately detect its coupling ratio. This study provided basis for the preparation of CIT antigen as well as the establishment of an for enzyme-linked immunosorbent assay （ELISA） for citrinin determination.

Comparison of Some Characteristics Between Phragmites communis and Its Salt Tolerant Variant

R5002_12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their molecular biological, physiological and biochemical characterizations. Five arbitrary primers were screened which showed differences in DNA amplified polymorphism between the variant and its wild line. Some new proteins appeared in the salt tolerant plant under salt stress. Electrophoresis of peroxidase and esterase also showed some differences in isozyme expression between them. The chlorophyll content of the variant was higher than that of the original variety, whether the plants were under salt stress or not.

Study on Release Properties of Slow Release Urea Coated by Industrial Lignin

This study aimed to investigate the release properties of slow release ure- a coated by industrial lignin and the influences of temperature, pH and post-ripening period on the release of nutrients in coated urea. According to the experimental results, the release of nutrients in coated fertilizers showed an S-shaped curve; on the 28th d, the nutrient release rate reached 73.77%; the differential solubility was 1.83%, and the theoretical release period was 50.25 d. The release of nutrients was significantly influenced by temperature. As the incubation temperature rose from 25 to 40 ℃, preliminary solubility increased from 8.03% to 16.24%; differential solubility increased from 1.83% to 1.88%; theoretical release period was reduced from 51.25 d to 45.55 d. The release of nutrients was promoted by H＋ and OH-. After they were placed at room temperature within 30 d, coated fertilizers exhibited the optimal release properties.

Study on Extraction Process of Phytoene from Tomato Paste

With tomato paste as raw material, the optimal extraction process of phy- toene from tomato paste was explored by orthogonal experiment on the basis of single-factor experiments. The results showed that the optimal extraction process for phytoene from tomato paste was as follows： extractant, acetone; solid to liquid ratio, 10：1; extraction time, 40 min; extraction temperature, 45 ℃. The developed extrac- tion process was stable, reasonable and feasible. Under the optimal extraction pro- cess, the extraction rate of phytoene reached 0.044 4%.

Synthesis of Phenylpropanoid Glycoside Analogs

The regioselective acylation of unprotected phenylethyl glucoside withcinnamoyl chloride leads to 6-OH cin-namoylated glucoside. In this manner, thirteen phenylpropanoidglycoside analogs were designed and prepared. Their structure was confirmed by ~1H NMR and ^(13)CNMR spectra.

设置素土垫层处理湿陷性黄土地基

设置素土垫层处理黄土地基，可减小垫层地基的压缩性和渗透性，还可减少或消除垫层地基的湿陷性。

Optimization of Conditions for the Extraction of Melanin from Walnut Husk

[Objective] This study was conducted optimize the conditions for the ex- traction of melanin from walnut husk. [Method] Melanin was isolated from walnut husk through alkali extraction and acid precipitation, and the factors that may influ- ence the extraction efficiency： temperature, duration, NaOH concentration and solid- liquid ratio were set at different levels. [Result] The optimal conditions for the ex- traction of melanin from walhut husk were temperature of 80 ℃, extraction duration of 120 min, NaOH concentration of 70 mol/L, and solid-liquid ratio of 1：20. [Conclu- sion] The optimal conditions for the extraction of melanin from walnut husk deter- mined in the present study will provide references for the development and utiliza- tion of natural melanin.

Influential Factors of House-purchasing Intention in Work construction Sites among Migrant Workers with a Case of Investigations on 203 Migrant Workers from Jiangxi Province

The research selected 13 factors from individual and family characters, construction site characters and work characters and analyzed the influences on the willingness of purchasing houses on construction sites with Logistic regression mod- el. The results showed that gender, age differences, personality, willingness on fami- ly land conversion, management of enterprises on construction sites, relatives and friends on construction lands, and Job satisfaction were approved by significant testing, but marital status, annual household income, the rate of migrant worker in a household, transportation in work city, and corporate reputation were not approved by significant testing.

Construction of a Tapetum-Specific and Tetracycline-Inducible System

A regulated gene expression system would offer the unique opportunity to study the gene physiological functions at different developmental stages. For realizing gene special expression in plant anther at given time, we constructed a new system that combined tetracycline- inducible elements with TA29 promoter, a tapetum-specific promoter of tobacco. The system was tested in transient GUS assay system by electroporation (gene gun) transformation of tobacco ( Nicotiana tabacum L. cv. Winsconsin 38) anther. In the absence of tetracycline as the inducer, no GUS activity was detected. However, strong GUS expression was observed in tapetum. tissue upon tetracycline induction, and little GUS activity was found outside the tapetum. Our results suggested that gene expression can be restricted to a specific tissue at the given time under the control of this new system, and this system would be a very useful tool for both basic plant biology research and biotechnological applications.

Symbiosis Analysis on Industrial Ecological System

Industrial ecological system is a sustainable mode of modern industry development. Industrial symbiosis, a sub-field of industrial ecology, engages traditionally separate industries in a collective approach, involving exchange of materials, energy, water, and/or by-products, to enhance competitive ability and environmental performance. To construct a symbiosis analysis method, this article employs a number of parameters embodying information about materials, energy and economics as the main essential parameters in system analysis and introduces symbiosis profit and symbiotic consumption elements as the economic indicators. A modeling and simulation program is designed using the agent-based modeling approach to simulate the evolvement of a hypothetical coal-based industrial system and the change of symbiosis conditions in the process of construction is examined. The simulation program built using the Swarm library, which is a freely available multi-agent simulation package, provides a useful demonstration for the symbiosis analysis method.

Environmental Regulation,Pollution Treatment Productivity,and Industrial Environmental TFP in China

Amidst growing environmental protection intensity by the Chinese government, this paper investigates the effects of environmental regulation on China＇s industrial pollution treatment productivity and environmental TFP. By estimating China＇s pollution treatment productivity between 2001 and 2008 and analyzing environmental regulation intensity and the effects of the relevant factors and pollution treatment productivity using panel data, this paper discovers that （1） pollution treatment productivity contributed a significant share of about 40% to industrial environmental TFP during the investigation period; （2） environmental regulation may not necessarily cause adverse impacts on pollution treatment efficiency and productivity but demonstrates a U-shaped relationship： when the share of pollution treatment cost in industrial value-added is above the range of 3.8%-5.1%, environmental regulation is likely to promote pollution treatment productivity and thus environmental TFP Judging by the estimation result, enhancing environmental protection and expediting the development of ecological civilization are conducive to China ＂s economic transition towards an intensive, efficient, circular, and sustainable development pattern. China＇s current industrial development has the capacity to tolerate a rather demanding level of pollution treatment and management and China needs to further rely on energy conservation and the environmental production industries to promote the progress of pollution treatment technologies.

Zhang Weishe n:A Pion eer in the Developme nt and In dustrial Production of Penicillin in New China

The research and development of penicillin started with dificulty before 1949 and achieved certain results.In 1951,after the founding of the People’ s Republic of China,Zhang Weishen,as the only Chinese scientist who had been trained and worked in a penicillin research and development center in the United States for many years,overcame many difficulties and returned to China.In 1953,with the efforts of Zhang Weishen and his colleagues,China realized the industrialized production of penicillin,alleviating the urgent needs of the masses.Antibiotics has also become the first discipline to achieve major scientific and technological achievements after the founding of the New China.In the mid-1950s,the technical breakthrough in the localization of lactose substitutes marked the localization of the raw materials of the penicillin-producing culture medium,which paved the way for the industrialized production of penicillin with Chinese characteristics.Antibiotics have become one of the most widely used and affordable drugs for hundreds of millions of patients in China,and China has since ended the humiliating history of the "Sick Man of East Asia".

Perioperative intensive insulin therapy using artificial endocrine pancreas in patients undergoing pancreatectomy

Perioperative glycemic control is important for reducing postoperative infectious complications. However, clinical trials have shown that efforts to maintain normoglycemia in intensive care unit patients result in deviation of glucose levels from the optimal range, and frequent attacks of hypoglycemia. Tight glycemic control is even more challenging in those undergoing pancreatic resection. Removal of lesions and surrounding normal pancreatic tissue often cause hormone deficiencies that lead to the destruction of glucose homeostasis, which is termed pancreatogenic diabetes. Pancreatogenic diabetes is characterized by the occurrence of hyperglycemia and iatrogenic severe hypoglycemia, which adversely effects patient recovery. Postoperatively, a variety of factors including surgical stress, inflammatory cytokines, sympathomimetic drug therapy, and aggressive nutritional support can also affect glycemic control. This review discusses the endocrine aspects of pancreatic resection and highlights postoperative glycemic control using a closed-loop system or artificial pancreas. In previous experiments, we have demonstrated the reliability of the artificial pancreas in dogs with total pancreatectomy, and its postoperative clinical use has been shown to be effectiveand safe, without the occurrence of hypoglycemic episodes, even in patients after total pancreatectomy. Considering the increasing requirement for tight perioperative glycemic control and the recognized risk of hypoglycemia, we propose the use of an artificial endocrine pancreas that is able to monitor continuously blood glucose concentrations with proven accuracy, and administer automatically substances to return blood glucose concentration to the optimal narrow range.

浅谈Geolord-AT空三格式导入PF的方法

像素工厂是新一代海量遥感影像处理系统,处理推扫式ADS数据"得心应手",但处理框幅数码影像如UCD,DMC等优势并不明显,尤其在加密方面。Geolord-AT是数字摄影测量工作站JX4C DPS的子项目,处理框幅数码影像如UCD,DMC等优势明显。故将二者结合,通过生产实践,提高了生产效率。

Toxic hepatitis in occupational exposure to solvents

The liver is the main organ responsible for the metabolism of drugs and toxic chemicals, and so is the primary target organ for many organic solvents. Work activities with hepatotoxins exposures are numerous and, moreover, organic solvents are used in various industrial processes. Organic solvents used in different industrial processes may be associated with hepatotoxicity. Several factors contribute to liver toxicity; among these are: species differences, nutritional condition, genetic factors, interaction with medications in use, alcohol abuse and interaction, and age. This review addresses the mechanisms of hepatotoxicity. The main pathogenic mechanisms responsible for functional and organic damage caused by solvents are: inflammation, dysfunction of cytochrome P450, mitochondrial dysfunction and oxidative stress. The health impact of exposure to solvents in the workplace remains an interesting and worrying question for professional health work.

Multiphase equilibrium modeling of oxygen bottom-blown copper smelting process

A computational thermodynamics model for the oxygen bottom-blown copper smelting process(Shuikoushan,SKS process)was established,based on the SKS smelting characteristics and theory of Gibbs free energy minimization.The calculated results of the model show that,under the given stable production condition,the contents of Cu,Fe and S in matte are71.08%,7.15%and17.51%,and the contents of Fe,SiO2and Cu in slag are42.17%,25.05%and3.16%.The calculated fractional distributions of minor elements among gas,slag and matte phases are As82.69%,11.22%,6.09%,Sb16.57%,70.63%,12.80%,Bi68.93%,11.30%,19.77%,Pb19.70%,24.75%,55.55%and Zn17.94%,64.28%,17.79%,respectively.The calculated results of the multiphase equilibrium model agree well with the actual industrial production data,indicating that the credibility of the model is validated.Therefore,the model could be used to monitor and optimize the industrial operations of SKS process.

Application of gap element to nonlinear mechanics analysis of drillstring

This paper presents a nonlinear finite element method to resolve the problem of the nonlinear contact between the drillstring and hole wall by using a Multi-directional Contact Gap Element (MCGE) contacting at appropriate positions in each beam element. The method was successfully applied to the Daqing Oil Field GP1 well. It was shown that the drillstring's contact resistance at any well depth could be obtained by calculations and that as the error in the calculation of the hole top load is below 10%, the calculation result can provide theoretical basis for the design and operation of drillstrings.

Improvement of prediction model for work roll thermal contour in hot strip mill

Though high accuracy of the thermal contour was obtained while adopting finite element method,it could not meet the real-time requirement.One-dimensional finite difference method could realize real-time control in the case of neglecting both the circumferential and radical heat exchange,but the over-simplified modes resulted in poor accuracy.And two-dimensional full explicit difference was also limited in practical application since its time step was restricted to keep the model stable.Consequently,a new method of alternating direction finite difference was introduced and discussed in the model's stability,calculating speed and precision.Specific work roll after one real rolling unit was researched,The result shows that error of temperature on work roll surface between measured and calculated values is within 5 ℃.The influence of rolling rhythm and strip width on thermal crown was also studied.The conclusion is verified theoretically and practically that it can maintain absolutely stable and meet the online requirement.

Tension leg platform project execution

The first floating platform concept design work for South China Sea is undergoing in DMAR's office now. This tension leg platform has potential to become the first advanced floating production platform project. Project execution is always a challenge for floating system. This paper focuses on the critical elements of project execution for tension leg platform,and studies potential implications to future oil and gas exploration in South China Sea. There are many factors affecting successful execution of floating system project,including technical issues, engineering management,interface management,etc. There are also failure examples of project execution in the industry. The author has participated 28 large detailed projects and has gained extensive experience on floating projects,with ample hands-on project experiences. A detailed tension leg platform project study example and discussions in depth are presented for future project execution in China deepwater development.

Numerical Modeling of Human Middle Ear Biomechanics Using Finite Element Method

An accurate finite element （ FE ） model of the human middle ear can provide better understanding of the mechanics of middle ear, and can be used for aiding the design of the implantable middle ear hearing devices. In this paper, a threedimensional （3D） FE model of the human middle ear was constructed, including the tympanic membrane, ossicular bones, and middle ear suspensory ligaments/museles. This model was constructed based on a complete set of computerized tomography section images of a healthy volunteer＇s left ear by reverse engineering technology. The validity of this model was confirmed by comparing the motions of the tympanic membrane and stapes footplate obtained by this model with published experimental measurements on human temporal bones. The result shows that the model is reasonable in predicting the biomechanics of the human middle ear.