素数及相应的素数基的性质

首先定义了素数和素数基数列,定义了有洞空间和无洞空间,然后讨论了有洞的充要条件;讨论了素数和素数基的有关性质:命题3.2、命题3.3;讨论了有洞和无洞之间的关系,得到了命题4.3、定理4.5、定理4.7.

Bombyx mori Pyridoxal Kinase cDNA Cloning and Enzymatic Characterization

Pyridoxal kinase （PLK） （EC 2.7.1.35） catalyzes the ATP-dependent phosphorylation of pyridoxal, generating pyridoxal-5＇-phosphate （PLP）, an important cofactor for many enzymatic reactions. Bombyx mori, similar to mammals, relies on a nutritional source of vitamin B6 to synthesize PLP. This article describes how a cDNA encoding PLK was cloned from Bombyx mori using the PCR method （GenBank accession number： DQ452397）. The cDNA has an 894 bp open reading frame and encodes a protein of 298 amino acid residues with a molecular mass of 33.1 k.Da. The amino acid sequence shares 48.6% identity with that of human PLK, and it also contains signature conserved motifs of the PLK family. However, the protein is 10 or more amino acids shorter than the PLK from mammals and plants, and several amino acid residues conserved in the PLK from mammals and plants are changed in the protein. The cDNA cloned was expressed successfully in Escherichia coli using the T7 promoter/T7 RNA polymerase expression system, and the crude extracts containing the expressed product were found to have strong PLK enzymatic activity with a value of 30 nmol/min/mg, confirming that the cDNA encodes the functional PLK of Bombyx mori. This is the first identification of a gene encoding PLK in insects.

Optimal choice of parameters for particle swarm optimization

The constriction factor method (CFM) is a new variation of the basic particle swarm optimization (PSO), which has relatively better convergent nature. The effects of the major parameters on CFM were systematically investigated based on some benchmark functions. The constriction factor, velocity constraint, and population size all have significant impact on the per- formance of CFM for PSO. The constriction factor and velocity constraint have optimal values in practical application, and im- proper choice of these factors will lead to bad results. Increasing population size can improve the solution quality, although the computing time will be longer. The characteristics of CFM parameters are described and guidelines for determining parameter values are given in this paper.

Melanocortin-1 receptor gene variants in four Chinese ethnic populations

There is strong relationship between melanocortin-1 receptor (MCIR) gene variants and human hair color and skin type. Based on a sequencing study of MC1R gene in 50 individuals from the Uygur, Tibetan, Wa and Dai ethnic populations, we discuss the occurrence of 7 mc1r variants consisting of 5 nonsynonymous sites (Val60Leu, Arg67Gln, Val92Met, Arg163Gln and Ala299Val) and 2 synonymous sites (C414T and A942G), among which C414T and Ala299Val were reported for the first time. Confirmation and analysis were the made of 122 individuals at three common point mutations (Val92Met, Arg163Gln, A942G) using PCR-SSCP. The frequency of Arg163Gln variant varies in the four ethnic populations, with percentage of 40%, 85.0%, 66. 2% and 72.7%, respectively, while those of Val92Met and A942G are roughly similar in these four populations. The different environments, migration and admixture of various ethnic groups in China might have impact on the observed frequency of Arg163Gln.

Safety assessment of Bifidobacterium longum JDM301 based on complete genome sequences

AIM： To assess the safety of Bifidobacterium/ongum （B. longum） JDM301 based on complete genome sequences. METHODS： The complete genome sequences of JDM301 were determined using the GS 20 system. Putative virulence factors, putative antibiotic resis- tance genes and genes encoding enzymes respon- sible for harmful metabolites were identified by blast with virulence factors database, antibiotic resistance genes database and genes associated with harmful metabolites in previous reports. Minimum inhibitory concentration of 16 common antimicrobial agents was evaluated by E-test RESULTS： JDM301 was shown to contain 36 genes as- sociated with antibiotic resistance, 5 enzymes related to harmful metabolites and 162 nonspecific virulence fac- tors mainly associated with transcriptional regulation, adhesion, sugar and amino acid transport. B. longum JDM301 was intrinsically resistant to ciprofloxacin, ami- kacin, gentamicin and streptomycin and susceptible to vancomycin, amoxicillin, cephalothin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, imi- penem and trimethoprim-sulphamethoxazol. JDM301 was moderately resistant to bacitracin, while an earlier study showed that bifidobacteria were susceptible to this antibiotic. A tetracycline resistance gene with the risk of transfer was found in JDM301, which needs to be experimentally validated. CONCLUSION： The safety assessment of JDM301 using information derived from complete bacterial ge- nome will contribute to a wider and deeper insight into the safety of probiotic bacteria.

Analysis of antibiotic resistance genes and their effects on marine environment

The application of overgrowth antibiotic resistance genes in marine research was discussed.Using the China National Knowledge Infrastructure(CNKI)network database to retrieve papers on resistance genes(n=30627)and antibiotic resistance genes(n=3277),the published trends,subject headings and other aspects of bibliometrics were analyzed.Literature in this field has increased rapidly in the past 38 years.55.3%of the papers were published in the last ten years,31.2%of the papers were published in the last five years.The relevance of research topics has increased in recent years,the current research mainly focus on antibiotics,resistance gene,antibiotic resistance gene,and drug resistance.Literature analysis is helpful to understand the overall development trend of overgrowth antibiotic resistance genes in marine research.

Algebraic-Based Nonbinary LDPC Codes with Flexible Field Orders and Code Rates

In this paper, we study the rank of matrices over GF(2~p),and propose two construction methods for algebraic-based nonbinary LDPC codes from an existing LDPC code, referred to as the original code. By multiplying all elements of each column of the binary parity-check matrix H corresponding to the original code with the same nonzero element of any field, the first class of nonbinary LDPC codes with flexible field order is proposed. The second method is to replace the nonzero elements of some columns in H with different nonzero field elements in a given field, and then another class of nonbinary LDPC codes with various rates is obtained. Simulation results show that the proposed nonbinary LDPC codes perform well over the AWGN channel with the iterative decoding algorithms.

AN IMPROVED RADIAL BASIS FUNCTION BASED METHOD FOR IMAGE WARPING

A new image warping method is proposed in this letter, which can warp a given image by some manual defined features. Based on the radial basis interpolation function algorithm, the proposed method can transform the original optimized problem into nonsingular linear problem by adding one-order term and affine differentiable condition. This linear system can get the steady unique solution by choosing suitable kernel function. Furthermore, the proposed method demonstrates how to set up the radial basis function in the target image so as to achieve supports to adopt the backward re-sampling technology accordingly which could gain the very slippery warping image. Theexperimental result shows that the proposed method can implement smooth and gradual image warping with multi-anchor points' accurate interpolation.

Practical Model of Sea Ice Thickness of Bohai Sea Based on MODIS Data

Sea ice thickness is one of the most important input parameters in the studies on sea ice disaster prevention and mitigation. It is also the most important content in remote sensing monitoring of sea ice. In this study, a practical model of sea ice thickness(PMSIT) was proposed based on the Moderate Resolution Imaging Spectroradiometer(MODIS) data. In the proposed model, the MODIS data of the first band were used to estimate sea ice thickness and the difference between the second-band reflectance and the fifth-band reflectance in the MODIS data was calculated to obtain the difference attenuation index(DAI) of each pixel. The obtained DAI was used to estimate the integrated attenuation coefficient of the first band of the MODIS at the pixel level. Then the model was used to estimate sea ice thickness in the Bohai Sea with the MODIS data and then validated with the actual sea ice survey data. The validation results showed that the proposed model and corresponding parameterization scheme could largely avoid the estimation error of sea ice thickness caused by the spatial and temporal heterogeneity of sea ice extinction and allowed the error of 18.7% compared with the measured sea ice thickness.

Influential Factors of Bucket Foundation for Offshore Wind Turbine

In this paper, the influential design thctors of wide-shallow composite bucket foundation for 3 MW off- shore wind turbine are systematically studied by numerical simulation. The results show that the bucket diameter is larger than 27 m in generak and the range of 7--12 m is appropriate for cylinder height. In particular the bucket foun- dation with diameter of 30 m and cylinder height of 10 m is suitable for most soils. Under ultimate loads, the bucket diameter and elasticity modulus of soil have major effects on the deibrmability of bucket foundation, while the influ- ence of friction coefficient between the bucket and soil is relatively slight.

Mast cell density and the context of clinicopathological parameters and expression of p185,estrogen receptor,and proliferating cell nuclear antigen in gastric carcinoma

AIM:To investigate the relationship between the mast cell density(MCD)and the context of clinicopathological parameters and expression of p185,estrogen receptor(ER), and proliferating cell nuclear antigen(PCNA)in gastric carcinoma. METHODS:Mast cell,p185,ER,and PCNA were detected using immunohistochemical S-P labeling method.Mast cell was counted in tissue of gastric carcinoma and regional lymph nodes respectively,and involved lymph nodes(ILN)were examined as usual. RESULTS:MCD was significantly related to both age and depth of penetration(x^2=4.688,P<0.05 for age and x^2=9.350, P<0.01 for depth of penetration)between MCD>21/0.03 mm^2 and MCD≤21/0.03 mm^2 in 100 patients;MCD in 1-6 ILN group patients was significantly higher than that in 7-15 ILN or>15 ILN group patients(u=6.881,8.055,P<0.01); There were significant differences intergroup in positive expression rate of p185,ER and PCNA between MCD>21/ 0.03 mm^2 and MCD≤21/0.03 mm^2 in 100 patients. CONCLUSION:Mast cell may have effect on inhibiting invasive growth of tumor,especially in the aged patients; The number of mast cells,in certain degree,may predicate the number of involved lymph nodes,which is valuable for assessment of prognosis;MCD was related to the expression of p185,ER,and PCNA in gastric carcinoma.It suggests that mast cell accumulation may inhibit the proliferation and the dissemination of the gastric carcinoma. INTRODUCTION Recently,many studies have reported on the association of mast cell with various tumorst.In several malignancies,mast cell has been found to correlate with growth,penetration and prognosis of tumor.Therefore,our study was undertaken to investigate the relationship between the mast cell density (MCD)and the context of clinicopathological parameters and expression of p 185,estrogen receptor(ER),and proliferating cell nuclear antigen(PCNA)in gastric carcinoma.

Hierarchical Scene Analysis Method for Audio Sensor Networks

Abstract： A hierarchical method for scene analysis in audio sensor networks is proposed. This meth-od consists of two stages： element detection stage and audio scene analysis stage. In the former stage, the basic audio elements are modeled by the HMM models and trained by enough samples off-line, and we adaptively add or remove basic ele- ment from the targeted element pool according to the time, place and other environment parameters. In the latter stage, a data fusion algorithm is used to combine the sensory information of the same ar-ea, and then, a role-based method is employed to analyze the audio scene based on the fused data. We conduct some experiments to evaluate the per-formance of the proposed method that about 70% audio scenes can be detected correctly by this method. The experiment evaluations demonstrate that our method can achieve satisfactory results.

New Pseudorandom Number Generator Artin-Sc hreier Tower for p = 5

The standard method to construct a finite field requires a primitive irreducible polynomial of a given degree. Therefore, it is difficult to apply for the construction of huge finite fields. To avoid this problem, we propose a new method to construct huge finite fields with the characteristic p = 5 by using an Artin-Schreier tower. Utilizing the recursive basis of the Artin-Schreier tower, we define a nmltiplication algorithm The algorithm can explicitly calculate the multiplication of two elements on the top finite field of this tower, without any primitive element. We also define a linear recurrence equation as an application, which produces a sequence of numbers, and call the new pseudorandom number generator Abstract Syntax Tree （AST） for p = 5. The experircental results show that our new pseudorandom number generator can produce a sequence of numbers with a long period.

Chaotic Characteristics of Bubbles Rising with Coalescences in Pseudoplastic Fluid

The chaotic characteristics of bubbles rising with accompanying coalescences in pseudoplastic aqueous carboxymethylcellulose sodium(CMC)solution were studied by means of smoothed pseudo Wigner-Ville distribution and Wigner-Hough distribution.The temporal signal of bubble passage was measured utilizing a photoconductive data acquisition system.As bubble coalescence occurred,the smoothed pseudo Wigner-Ville distribution of the signal revealed that the signal could be divided into low-frequency and high-frequency ranges and the transition range according to the distribution feature of frequency domain,which reflected eddy motion of fluid,high frequency fluctuations of fluid velocity and other random components measured in the signal,and bubbles rising accompanied with coalescences,respectively.However,bubble coalescence occurred in the lower position and the frequency range of bubbles motion became wide under higher gas flowrate,while the frequency range of bubbles motion became narrow when the CMC concentration increased.The typical dynamics of bubbles motion,such as periodicity,bifurcation and chaos,could be easily found in terms of the Wigner-Hough distribution.

Numerical Classification of Brevibacterium and Related Genera Using Linocin M18 Bacteriocin

Fifty bacterial strains isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients were identified to 22 species and the following genera： Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus and classified numerically using a set of 52 phenetic characteristics, using simple matching coefficient （Ssm） and clustering method of unweighted average linkage between groups by SPSS program. They were also grouped to 7 main clusters and 29 sub-clusters in the hierarchical tree. Twelve isolates of the different species from the genera Brevibacterium, Arthrobacter, Corynebacterium, Kocuria, Rhodococcus, Rothia were selected from the taxonomic clusters and probed for lin gene by peR. One species Kocuria rhizophila which inhibited most of the test organism did not have lin gene in the chromosome while the species Corynebacterium glucuronolyticum, Arthrobacter comminsii, Arthrobacter oxydans have the lin gene. Our results establish a wide distribution of the structural gene encoding this Iinocin M 18 within coryneform bacteria and also in the genus Kocuria.

Hilbert＇s Foundations Remain Intact

Some of the seemingly important contributions made by Russell, Cantor and Godel regarding the foundations of mathematics lose credibility when addressed at a more fundamental level which assumes language consists of symbols devoid of what they mean. Such an approach can reveal neglected assumptions which are of consequence when made explicit and which, in particular, mean that Hilbert in fact had no need to heed Godel＇s revolutionary results regarding the foundations of mathematics.

Vibrational Structure of Selected Compounds Derived from Biomass： Lignin Dimers, Selected Aldopentoses and Aldohexoses

The production of chemicals from biomass is a very challenging process due to its diverse chemical composition. Lignin, cellulose and hemicellulose are the three main biopolymers of wood biomass, with cell walls ＆plant origin. Lignin has been chosen for the present studies due to its range of different linkages and structures. The present work involved a computational study of the most dominant lignin dimers and their vibrational structures, based on the Density Functional Theory method. Full geometry optimization of the compartments used the StoBe code with cluster model and non-local functional （RPBE） approach. The calculations of the vibrational frequencies were performed with harmonic approximations as well as an anharmonicity fit in the Morse potential function, as implemented in the StoBe code. In the case oflignin, the calculations included three different precursors based on： coumaryl alcohol, coniferyl alcohol and sinapyl alcohol. To represent the cellulose and hemicellulose derivatives, selected aldopentoses and aldohexoses （arabinose, xylose, glucose, galactose, and mannose） were considered. Presented here are the theoretical investigations for a variety of biomass derived compounds, to give the possibility of obtaining a theoretical VBD （Vibrations Basis Database） for experimental spectra interpretation. Such a database could be further used in the preliminary composition assessment of biomass derived substrates, which will be discussed here in more detail.

Molecular Evolution of the Cytochrome b Gene in Geographically Isolated Populations of Oryzias Latipes

The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5＇ and 3＇ ends was 1,143 base pairs （bp） and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control （Hd-rR strain） and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 （K-I I strain） and AB480879 （HS strain）.

Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.

Sequencing Analysis of Myostatin Gene （MSTN） for Meat Cattle in Mongolia

In developed countries, study on special or candidate genes, which are useful for identifying species, breed and productivity of livestock, was conducted at high level and the results have already been used in practice. Such advanced technology and innovation that we are facing is necessary to adopt in Mongolia. In this study, the myostatin gene （MSTN） was investigated as a candidate gene for meat animal in Mongolian breeds of cattle. The conventional phenol-chloroform method and FavorPrepTM tissue DNA extraction kit were used for DNA isolation, and the polymerase chain reaction （PCR） and sequencing analysis were used for further study. The nucleotide sequences of MSTN gene from Selenge, Kazakh white head breeds and Mongolian cattle were sequenced and reported on the DDBJ/EMBL/GenBank database （LC142726, LC146648, LC146649）, and Selenge breed showed the result of single nucleotide mutation in MSTN gene.