This study discusses the application of the regulatory focus of the individual motivation systems to green public service advertisements advocating environmental protection. First, it aims to determine whether the pre...This study discusses the application of the regulatory focus of the individual motivation systems to green public service advertisements advocating environmental protection. First, it aims to determine whether the previously proposed regulatory fit theory can be applied to public service advertisements with characteristics different from those of commercial advertisements. Second, it aims to understand how to design messages to achieve the best effect on audiences with different foci. This study takes the regulatory focus theory as the basis and green public service advertisements as the films. After adopting 2 ~ 2 ~ 2 three-factor design to test the interaction relations among the individual regulatory focus, message regulatory focus, and message framing. The results from the 217 college respondents indicate that the audience is influenced by regulatory focus when receiving messages from public service advertisements, which is similar to the influence of commercial advertisements. That is, when audience with a promotion focus, films with promotion focus and positive framing have a best advertisement effect. On the other hand, the audience with a prevention focus, films with prevention focus and negative framing also has a better advertisement effect.展开更多
[ Objective] To study growth-promoting effects of liposome-encapsulated porcine somatotropin （pST） and its sustained-release effects in mice. [ Method] The mice were randomly divided into experimental groups and con...[ Objective] To study growth-promoting effects of liposome-encapsulated porcine somatotropin （pST） and its sustained-release effects in mice. [ Method] The mice were randomly divided into experimental groups and control group. The mice in the experimental groups were respective- ly administrated with pST and liposome-encapsulated pST via intraperitoneal injection, and those in the control group were administrated with saline. They were weighed on Day 7, 14 and 28, and the growth rate was calculated. [ Result] The growth of mice had significant difference between the experiment groups and control group （P 〈 0.05）, which indicated that the pST and liposome-encapsulated pST had growth-promoting effects in mice, and the liposome-encapsulated pST had sustained-release effects. [Condusion] The liposome-encapsulated pST can promote the growth of animal and its sustained-release peried is 7 d.展开更多
Objective To investigate the regulatory effects of somatostatin (SST) and octreotide (OCT) on the extracellular matrixes (ECM) metabolism of rat hepatic stellate cells (HSCs). Methods Being treated with different conc...Objective To investigate the regulatory effects of somatostatin (SST) and octreotide (OCT) on the extracellular matrixes (ECM) metabolism of rat hepatic stellate cells (HSCs). Methods Being treated with different concentrations of SST or OCT, the mRNA levels of collage type I, III and the expression of collagen, matrix metalloproteinase-1(MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) in activated HSCs were assessed by in situ hybridization (ISH), 3H-proline incorporation and immunocytochemistry, respectively. In addition, levels of hyaluronic acid (HA), laminin (LM), and procollagen type III (PCIII) in the culture supernatant of HSCs were also detected by means of enzyme-linked immunosorbent assay (ELISA). ResultsBoth SST and OCT markedly down-regulated the transcription of collagen type I, III in activated HSCs within the range of 10-6-10-7 mol/L and 10-5-10-7 mol/L, respectively (P<0.05). SST-treated groups of 10-6-10-7 mol/L and OCT-treated groups of 10-5-10-7 mol/L also demonstrated statistically decreased production of collagen, HA, LM, PCIII in a dose-dependent way (P<0.05). Furthermore, HSCs cocultured with SST (10-6 mol/L) or OCT (10-5-10-6 mol/L) exhibited TIMP-1 levels much lower than that of normal control (P<0.05), which resulted in an elevated ratio of MMP/TIMP. Conclusion SST and its analog may play a negative regulatory role in the ECM metabolism of HSCs. Their effect may be benefit for prevention and therapy of hepatic fibrosis.展开更多
文摘This study discusses the application of the regulatory focus of the individual motivation systems to green public service advertisements advocating environmental protection. First, it aims to determine whether the previously proposed regulatory fit theory can be applied to public service advertisements with characteristics different from those of commercial advertisements. Second, it aims to understand how to design messages to achieve the best effect on audiences with different foci. This study takes the regulatory focus theory as the basis and green public service advertisements as the films. After adopting 2 ~ 2 ~ 2 three-factor design to test the interaction relations among the individual regulatory focus, message regulatory focus, and message framing. The results from the 217 college respondents indicate that the audience is influenced by regulatory focus when receiving messages from public service advertisements, which is similar to the influence of commercial advertisements. That is, when audience with a promotion focus, films with promotion focus and positive framing have a best advertisement effect. On the other hand, the audience with a prevention focus, films with prevention focus and negative framing also has a better advertisement effect.
文摘[ Objective] To study growth-promoting effects of liposome-encapsulated porcine somatotropin （pST） and its sustained-release effects in mice. [ Method] The mice were randomly divided into experimental groups and control group. The mice in the experimental groups were respective- ly administrated with pST and liposome-encapsulated pST via intraperitoneal injection, and those in the control group were administrated with saline. They were weighed on Day 7, 14 and 28, and the growth rate was calculated. [ Result] The growth of mice had significant difference between the experiment groups and control group （P 〈 0.05）, which indicated that the pST and liposome-encapsulated pST had growth-promoting effects in mice, and the liposome-encapsulated pST had sustained-release effects. [Condusion] The liposome-encapsulated pST can promote the growth of animal and its sustained-release peried is 7 d.
基金the Scientific and Technological Committee of Shanghai (974119003)
文摘Objective To investigate the regulatory effects of somatostatin (SST) and octreotide (OCT) on the extracellular matrixes (ECM) metabolism of rat hepatic stellate cells (HSCs). Methods Being treated with different concentrations of SST or OCT, the mRNA levels of collage type I, III and the expression of collagen, matrix metalloproteinase-1(MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) in activated HSCs were assessed by in situ hybridization (ISH), 3H-proline incorporation and immunocytochemistry, respectively. In addition, levels of hyaluronic acid (HA), laminin (LM), and procollagen type III (PCIII) in the culture supernatant of HSCs were also detected by means of enzyme-linked immunosorbent assay (ELISA). ResultsBoth SST and OCT markedly down-regulated the transcription of collagen type I, III in activated HSCs within the range of 10-6-10-7 mol/L and 10-5-10-7 mol/L, respectively (P<0.05). SST-treated groups of 10-6-10-7 mol/L and OCT-treated groups of 10-5-10-7 mol/L also demonstrated statistically decreased production of collagen, HA, LM, PCIII in a dose-dependent way (P<0.05). Furthermore, HSCs cocultured with SST (10-6 mol/L) or OCT (10-5-10-6 mol/L) exhibited TIMP-1 levels much lower than that of normal control (P<0.05), which resulted in an elevated ratio of MMP/TIMP. Conclusion SST and its analog may play a negative regulatory role in the ECM metabolism of HSCs. Their effect may be benefit for prevention and therapy of hepatic fibrosis.
