The aim of this study was to investigate the interaction between polymers polyApolyU (RNA) and two general anti-viral drugs with anti-HIV-1 activities. Tm experiment showed that the compounds had interacted with RNA, ...The aim of this study was to investigate the interaction between polymers polyApolyU (RNA) and two general anti-viral drugs with anti-HIV-1 activities. Tm experiment showed that the compounds had interacted with RNA, and CD spectra also observed the changes of RNA conformation induced by the compounds. Cytometric flow analysis indicated that ribavirin and DII-18-2 could decrease the percentage of hypodiploid cells, especially ribavirin.展开更多
Objective： The aim of the study was to investigate the effect of Casticin on proliferation inhibition of human cervical cancer HeLa cells in vitro and to unravel the associated mechanisms. Methods： Human cervical He...Objective： The aim of the study was to investigate the effect of Casticin on proliferation inhibition of human cervical cancer HeLa cells in vitro and to unravel the associated mechanisms. Methods： Human cervical HeLa cells were cultured in vitro. The inhibitory effect of Casticin on the viability of human cervical cancer HeLa ceils was evaluated by the MTT assay. The colony formation ability was detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry. The protein expression levels were analyzed by Western blot. Results： Casticin significantly inhibited the growth of human cervical cancer HeLa cells in a dose- and time-dependent manner, and the IC50 was 2.82 μg/mL. The colony-forming rate was reduced drastically compared with control group （P 〈 0.05）. The cells were markedly arrested at G2/M phase after the treatment of Casticin for 48 h. Western blot showed that the expression of p21 protein was up-regulated and protein level of Cyctin B1 was depressed by Casticin in a concentration dependent manner. Conclusion： Casticin could inhibit the cell growth and lead to cell arrest in human cervical cancer HeLa cells, and the down-regulation of Cyclin B1 protein expression and activation of p21 protein might contribute to Casticin induced cell arrest in human cervical cancer HeLa cells.展开更多
Some famous local spices were selected to determine their potentials in terms of their aroma and utilization. They included Tea Bush （Ocimum basilicum）, Guinea Black Pepper （Piper guineense）, African Pepper （Xylo...Some famous local spices were selected to determine their potentials in terms of their aroma and utilization. They included Tea Bush （Ocimum basilicum）, Guinea Black Pepper （Piper guineense）, African Pepper （Xylopia aethiopica）, Alden Tree （Tetrapleura tetraptera） and the African Meg Nut or Onion Tree （Blighia unijugata）. Essential oils were extracted from the samples and used for aroma absorption test in a uv-visible spectrometer V2.03. The intensity of aroma was indicated at some peak values of absorption at particular wavelengths. The compounds responsible for the aroma at this peak values were identified by matching their wavelengths with those of known compounds at similar wavelengths of absorption. Thus, a compound such as Quinoline that has hot strong aroma and little bleaching effect was proved to be present in Guinea black pepper ;at the wavelength of 313.0 nm. Benzene with hot strong aroma and flammable effect was also indicated in this spice at the wavelength of 253.0 rim. Proximate analysis carried out showed that these spices have high nutritive values. Some of them such as Guinea black pepper, African Nutmeg and Ethiopian （African） pepper had high ether extract of 20.01, 25.97 and 31.25 respectively, and could be regarded as oil seeds, they contain much of essential oils.展开更多
Using heptane, toluene, and tetrahydrofuran （THF） as eluant, asphaltenes were fractionated into five fractions based on their polarity and solubility. The molecular composition of polar heteroatom species in both as...Using heptane, toluene, and tetrahydrofuran （THF） as eluant, asphaltenes were fractionated into five fractions based on their polarity and solubility. The molecular composition of polar heteroatom species in both asphaltene and its fractions were ana- lyzed by negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry （ESI FT-ICR MS）. The application of UV-vis spectrometer in characterizing asphaltene composition and measuring asphaltene concentration was discussed. About 11.9 wt% asphaltene components adsorbed permanently on silica gel in the extrography column after exces- sive elution with various solvents. In negative FT-ICR MS, the mass spectra show that acidic and neutral nitrogen-containing compounds such as N1 and NIS1 mainly existe in the first three less polar fractions, while oxygen-containing compounds such as 02, O2S, O2S2, 03, and 04 show high relative abundance in more polar fractions. These results suggest oxygen-containing compounds have stronger adsorption ability with silica gel. It was observed that the double bond equivalence （DBE） distribu- tion of N1 class species in the fractions shifted to higher values while the carbon number shifted to smaller numbers as polarity of fractions increased. This indicates that acidic and neutral Nl compounds with longer carbon chain and less aromaticity have less polarity compared with those with shorter carbon chain and stronger aromaticity. UV-vis absorbance indicats that fractions containing the most aromatic and most polar asphaltene have better absorbance at long wavelength, while the fractions that consist of least aromatic and least polar asphatlenes show high absorbance at short wavelength.展开更多
基金This project was supported by the National Natural Science Foundation of China Doctoral Program Foundation of China.
文摘The aim of this study was to investigate the interaction between polymers polyApolyU (RNA) and two general anti-viral drugs with anti-HIV-1 activities. Tm experiment showed that the compounds had interacted with RNA, and CD spectra also observed the changes of RNA conformation induced by the compounds. Cytometric flow analysis indicated that ribavirin and DII-18-2 could decrease the percentage of hypodiploid cells, especially ribavirin.
基金Supported by a grant from Hunan Provincial Health Department Research Fund (No.B2007-116)
文摘Objective： The aim of the study was to investigate the effect of Casticin on proliferation inhibition of human cervical cancer HeLa cells in vitro and to unravel the associated mechanisms. Methods： Human cervical HeLa cells were cultured in vitro. The inhibitory effect of Casticin on the viability of human cervical cancer HeLa ceils was evaluated by the MTT assay. The colony formation ability was detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry. The protein expression levels were analyzed by Western blot. Results： Casticin significantly inhibited the growth of human cervical cancer HeLa cells in a dose- and time-dependent manner, and the IC50 was 2.82 μg/mL. The colony-forming rate was reduced drastically compared with control group （P 〈 0.05）. The cells were markedly arrested at G2/M phase after the treatment of Casticin for 48 h. Western blot showed that the expression of p21 protein was up-regulated and protein level of Cyctin B1 was depressed by Casticin in a concentration dependent manner. Conclusion： Casticin could inhibit the cell growth and lead to cell arrest in human cervical cancer HeLa cells, and the down-regulation of Cyclin B1 protein expression and activation of p21 protein might contribute to Casticin induced cell arrest in human cervical cancer HeLa cells.
文摘Some famous local spices were selected to determine their potentials in terms of their aroma and utilization. They included Tea Bush （Ocimum basilicum）, Guinea Black Pepper （Piper guineense）, African Pepper （Xylopia aethiopica）, Alden Tree （Tetrapleura tetraptera） and the African Meg Nut or Onion Tree （Blighia unijugata）. Essential oils were extracted from the samples and used for aroma absorption test in a uv-visible spectrometer V2.03. The intensity of aroma was indicated at some peak values of absorption at particular wavelengths. The compounds responsible for the aroma at this peak values were identified by matching their wavelengths with those of known compounds at similar wavelengths of absorption. Thus, a compound such as Quinoline that has hot strong aroma and little bleaching effect was proved to be present in Guinea black pepper ;at the wavelength of 313.0 nm. Benzene with hot strong aroma and flammable effect was also indicated in this spice at the wavelength of 253.0 rim. Proximate analysis carried out showed that these spices have high nutritive values. Some of them such as Guinea black pepper, African Nutmeg and Ethiopian （African） pepper had high ether extract of 20.01, 25.97 and 31.25 respectively, and could be regarded as oil seeds, they contain much of essential oils.
文摘Using heptane, toluene, and tetrahydrofuran （THF） as eluant, asphaltenes were fractionated into five fractions based on their polarity and solubility. The molecular composition of polar heteroatom species in both asphaltene and its fractions were ana- lyzed by negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry （ESI FT-ICR MS）. The application of UV-vis spectrometer in characterizing asphaltene composition and measuring asphaltene concentration was discussed. About 11.9 wt% asphaltene components adsorbed permanently on silica gel in the extrography column after exces- sive elution with various solvents. In negative FT-ICR MS, the mass spectra show that acidic and neutral nitrogen-containing compounds such as N1 and NIS1 mainly existe in the first three less polar fractions, while oxygen-containing compounds such as 02, O2S, O2S2, 03, and 04 show high relative abundance in more polar fractions. These results suggest oxygen-containing compounds have stronger adsorption ability with silica gel. It was observed that the double bond equivalence （DBE） distribu- tion of N1 class species in the fractions shifted to higher values while the carbon number shifted to smaller numbers as polarity of fractions increased. This indicates that acidic and neutral Nl compounds with longer carbon chain and less aromaticity have less polarity compared with those with shorter carbon chain and stronger aromaticity. UV-vis absorbance indicats that fractions containing the most aromatic and most polar asphaltene have better absorbance at long wavelength, while the fractions that consist of least aromatic and least polar asphatlenes show high absorbance at short wavelength.
