高原护肤霜的制备及抗紫外线作用研究

目的：制备适合高原地区使用的防晒、防裂护肤霜。方法：选用疏水性强、润滑涂展性好、能抗高寒气流的聚甲基硅氧烷为主要基质，壬基酚聚氧乙烯醚（ Ｏ Ｐ）为主要乳化剂，附加其他多种防晒护肤成分制得 Ｏ／ Ｗ 型高原护肤霜。抗紫外线实验采用氯丙嗪在光照下发生光毒反应模型，从光毒反应的减轻程度反映霜剂抗紫外线作用的强弱。结果：动物实验表明，高原护肤霜具有显著的抗紫外线效果，优于擦脸油产品，且对皮肤、角膜等均无刺激性和过敏反应发生。结论：高原护肤霜具有可靠的防晒作用，建议作为一种特殊防护用品装备部队使用。

海洋生物提取物抗紫外线作用研究

目的从海洋生物组织中提取制备对紫外线具有较强吸收作用的成分,为建立紫外线损伤的防护提供新方法。方法高压液相纯化海洋生物组织的提取物,观察其紫外吸收特征并绘制曲线,优选出有较强紫外吸收作用的样品,采用细胞培养法观察其对菌落及细胞的保护作用。结果优选出提取物的紫外吸收波长范围在220～300nm,且保持了较高的紫外吸收能力;经菌落抗紫外线和细胞抗紫外线实验证实,该提取物对菌落和细胞具有明显的紫外防护作用,菌落和细胞计数与对照组比较差异均有显著性(P<0.05)。结论本研究制备的海洋生物提取物具有明显的紫外防护作用。

慢性光线性皮炎紫外线作用光谱的研究

目的测定慢性光线性皮炎(CAD)紫外线作用光谱。方法以SUV1000型日光紫外模拟器为光源,测定131例疑诊和13例已确诊CAD患者的长波紫外线(UVA)和中波紫外线(UVB)最小红斑量(MED)。结果131例患者中,85例确诊为CAD。85例患者中89.41%UVA-MED降低,范围1-20 J/ cm2;69.41%UVB-MED降低,范围2.8-20mJ/cm2;30.58%单一UVA-MED下降,10.59%单一UVB-MED 下降。13例以前已经确诊为CAD的患者,重新测定MED发现,3例发病时MED在正常范围,9例作用光谱发生了改变。结论LVA在CAD作用光谱中占有重要地位CAD的作用光谱具有波动性,光敏感性增强可从一个波段变化至另一波段。

中波紫外线对成纤维细胞的损伤作用

目的:探讨中波紫外线(UVB)对成纤维细胞的损伤作用,建立细胞损伤模型。方法:采用UVB灯照射小鼠成纤维细胞株NIH3T3,MTT法测细胞存活率,流式细胞术检测细胞周期、凋亡和H2 O2 的生成。结果:0 .2～3.0 k J·m- 2 U VB照射后2 4 h细胞存活率明显降低(P<0 .0 5或0 .0 1) ;1.5 k J·m- 2 UVB照射后4、8、18和2 4 h细胞G1 期细胞百分数升高,凋亡小体增多;1.5 k J·m- 2 U VB照射后8h H2 O2 生成增多,与0、4、18和2 4 h组比较差异有显著性(P<0 .0 5 )。结论:U VB照射可诱导成纤维细胞出现Gl 期阻滞、细胞凋亡及H2 O2 生成增多。

微小RNA-133b对紫外线诱导的晶状体上皮细胞凋亡的抑制作用及其调控机制

背景 研究证实紫外线B照射是白内障发生的主要原因之一，其机制与晶状体上皮细胞（LECs）凋亡有关。微小RNA-133b（miR-133b）参与氧化应激诱导的LECs凋亡的调控过程，但其是否参与紫外线诱导白内障的发病过程及其机制尚未阐明。 目的 观察miR-133b对紫外线诱导白内障LECs凋亡的抑制作用及其调控机制。 方法 将20只8周龄SPF级C57BL/6小鼠采用随机数字表法分为白内障模型组和正常对照组，其中白内障模型组小鼠用波长302 nm的紫外线直接照射眼部5 min，照射强度为300 W/cm2，每天照射1次，共照射1周；正常对照组小鼠不给于任何干预。于末次照射后24 h处死各组小鼠并摘出10只眼球以制备全眼球切片。取人LECs细胞系（SRA01/04）紫外线照射25 min，并继续培养4 h作为紫外线照射组，正常对照组细胞不作任何干预。取紫外线照射模型组细胞接种于96孔板并分为miR-133b模拟物组、模拟物阴性对照组、miR-133b抑制物组和抑制物对照组，分别用lipofectamine2000联合50 nmol/L miR-133b模拟物、miR-133b模拟物对照剂、100 nmol/L miR-133b抑制物或miR-133b抑制物对照剂瞬时转染细胞。采用实时荧光定量PCR法检测小鼠晶状体组织和不同转染组人LECs中miR-133b mRNA及其预测靶基因BCL2L2 mRNA的表达以评估转染效率；采用TUNEL凋亡检测试剂盒检测小鼠晶状体组织中LECs和不同转染组人LECs的凋亡情况。 结果 正常对照组小鼠晶状体前囊膜LECs排列规则，未发现TUNEL染色阳性细胞，白内障模型组小鼠LECs排列稀疏，可见凋亡细胞呈红色荧光。紫外线照射组细胞凋亡率为（43.90±9.30）%，明显高于正常对照组的（1.08±0.49）%，差异有统计学意义（t＝－7.963，P＝0.015）。白内障模型组小鼠晶状体组织和紫外线照射组细胞中miR-133b mRNA的相对表达量分别低于正常对照组小鼠和正常人LECs，差异均有统计学意义（t＝－2.958，P＝0.042；t＝－6.195，P＝0.003）。白内障模型组小鼠晶状体组织和紫外线照射组细胞中BCL2L2 mRNA的相对表达量分别高于正常对照组小鼠和正常人LECs，差异均有统计学意义（t＝3.761，P＝0.020；t＝12.437，P＝0.000）。miR-133b模拟物组人LECs中miR-133b mRNA的相对表达量明显高于miR-133b模拟物对照组，而BCL2L2 mRNA的相对表达量明显低于miR-133b模拟物对照组，差异均有统计学意义（t＝10.883、－5 927.617，均P〈0.01）；miR-133b抑制物组人LECs中miR-133b mRNA的相对表达量明显低于miR-133b抑制物对照组，而BCL2L2 mRNA的相对表达量明显高于miR-133b抑制物对照组，差异均有统计学意义（t＝－1 606.622、17.556，均P〈0.01）。miR-133b模拟物组LECs凋亡率明显低于miR-133b模拟物对照组[（17.55±4.24）%与（43.62±9.19）%]，miR-133b抑制物组LECs凋亡率为（78.23±12.42）%，明显高于miR-133b抑制物对照组的（48.01±9.68）%，差异均有统计学意义（t＝－4.462，P＝0.011；t＝3.324，P＝0.029）。 结论 miR-133b可防止紫外线照射所致的白内障的形成，其机制可能与靶向负性调控BCL2L2基因从而调控LECs的凋亡过程有关。

一起紫外线伤及多人的护理体会

目的防止紫外线再次误伤室内人员。方法紫外线灯开关应该采用荧光警示装置,以避免在黑暗中误开。结果目前医院里紫外线灯存在不合理安装现象,医院对紫外线灯管理差,入院宣教不到位,病人、陪护不了解紫外线灯的作用,不明白紫外线的副作用。结论加强病人、陪护的入院宣教;加强对紫外线灯的管理;加强护理人员的岗前培训,提高责任心;改善目前医院紫外线灯的不合理安装。

二苯乙烯苷可抑制中波紫外线诱导的人皮肤成纤维细胞光老化

目的:研究二苯乙烯苷(TSG)对紫外线B(UVB)诱导的人皮肤成纤维细胞(HSF)应激性提早衰老的作用及可能的机制。方法:UVB小剂量、多次照射HSF,每次照射完毕后分别用0.02、0.10、0.50 mmol/L浓度的TSG处理。实验分为6组:空白对照组、模型对照组、UVB+0.02 mmol/L TSG组、UVB+0.10 mmol/L TSG组、UVB+0.50 mmol/L TSG组、TSG对照组。采用细胞计数法(CCK-8)检测细胞增殖活性;细胞化学染色法检测衰老相关β-半乳糖苷酶(SA-β-gal)表达;TBA法、WST-1法测定细胞丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;ELISA法测定细胞上清液中基质金属蛋白酶-1(MMP-1)的含量变化。结果:与空白对照组比较,模型对照组细胞增殖活性减弱(P<0.05),SA-β-gal染色呈强阳性,细胞裂解液中SOD活性减弱、MDA含量增加,MMP-1浓度升高(P<0.05或P<0.01)。与模型对照组比较,UVB+各浓度TSG组细胞增殖活性改善(均P<0.05),SA-β-gal染色阳性率下降,细胞裂解液中SOD活性增强、MDA含量减少,MMP-1浓度减小(P<0.05或P<0.01)。结论:TSG可以在一定程度上抑制UVB诱导的HSF应激性提早衰老,该作用可能与改善氧化应激、抑制MMP-1高表达有关。

LRP16基因抗紫外线DNA损伤作用的研究

目的根据基因编码序列推测其氨基酸序列,预测LRP16基因编码蛋白的功能并通过实验加以证实。方法利用GeneBank数据库获取LRP16基因序列,然后推测出该基因编码蛋白质的一级结构,对该序列蛋白结构域的同源性进行搜索;将LRP16基因ORF插入pcDNA3.1,构建LRP16基因的真核表达质粒,采用Superfect稳定转染急性粒细胞白血病细胞系HL60,筛选稳定表达LRP16基因的细胞;以紫外灯照射筛选后有HL60稳定过表达的HL60细胞和对照细胞;采用单细胞凝胶电泳技术检测有LRP16过表达对HL60细胞增殖及DNA损伤后修复的影响。结果在LRP16基因理论蛋白序列的第148至315氨基酸残基之间具有与人类组蛋白H2A1C末端相类似的同源序列hismacro、COG2110和A1pp,同源性高达80%以上。LRP16基因的过表达具有抗紫外线引起HL60细胞DNA损伤和增强损伤后的修复作用。结论LRP16基因编码蛋白可能具有抗紫外线的DNA损伤作用。

光量子血液疗法综合治疗肺部感染40例临床观察

光量子血液疗法综合治疗肺部感染４０例临床观察米贵玲我院自１９９３年３月～１９９５年５月，试用光量子血液疗法（简称ＵＢＩ）综合治疗该病，取得满意疗效，现报道如下。１．资料与方法１．１病例分组治疗组４０例，均为男性。年龄５５～８０岁，平均年龄７２岁。病程...

夏季防晒必备的食物

（1）红色或黄色的蔬果红色或黄色的蔬果或深绿色的叶菜，可以增强皮肤抵抗力，有效隔离紫外线。红色或黄色蔬果有芒果、木瓜、地瓜、南瓜、胡萝卜、番茄等食物。虽说这些食物具有很好的防紫外线作用，但是吃得过多也没有益处，比如胡萝卜吃得过多就会产生毒性，并能使皮肤变得暗黄。停止食用后，也得需要一段时间慢慢恢复。

Effects of Elevated Solar UV-B Radiation from Ozone Depletion on Terrestrial Ecosystems

In the last three decades much researchhas been carried out to investigate the biologicaleffects of a thinning stratospheric ozone layeraccompanied by an enhanced level of solarultraviolet-B radiation at the Earth’s surface.Enhanced UV-B radiation affects ecosystems in manyways directly and indirectly. The responses can bebiochemical, physiological, morphological oranatomical, and the direction of the response can varybetween different species, communities andecosystems. In this paper we firstly introduce generalconcepts, and methods for measuring the ecologicaleffects of UV-B radiation. Secondly, we provide anoverview interpretation of the effects of enhancedUV-B on terrestrial ecosystems from recent studies.These studies include effects of UV-B on growth andreproduction, composition of communities,competitive balance, decomposition of litter, andinteractions with other factors etc. Finally, werecommend future research directions to identify theeffects of elevated UV-B radiation on ecosystems inChina.

Investigation of bystander effect in Molt-4 cells induced by ultraviolet ray

Objective： To find out whether ultraviolet ray, a kind of non-ionic ray, could cause the bystander effect, the UV exposed MOLT-4 cells had been investigated. Methods： Two experiment groups were carried out, in which cells were culture and treated at two concentrations： 2 × 10^5/mL and 5 × 10^5/mL. All other treatments were the same. Part of the cells was labeled with DID and exposed to UV ray for 40 s as effect cells; other cells was untreated as bystander cells. Then, the cells were co-cultured and harvested at 4 h interval over a period of 24 h. Annexin V-FITC/PI assay was used to evaluate the bystander effect in bystander cells co-cultured with effected cells. Laser confocal microscope method was used to observe the morphologic changes of the bystander cells. Results： The percentage of cells undergoing apoptosis in the bystander cells was increased over time compared with the control group. They were 6.84%, 8.09%, 9.88%, 17.64%, 17.43%, 30.99% and 37.93% respectively in 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h. When observed by laser scanning confocal microscope, the bystander cells show some classic character of apoptosis such as chromosome condense, phosphatidylsedne transfer and formation of apoptotic bodies. Conclusion： Bystander effect is significant in un-irradiated bystander MOLT-4 cells when co-cultured with UV exposed cells.

Antioxidant activity of phytosynthesized biomatrix-loaded noble metallic nanoparticles

Biofabrication of noble monometallic platinum nanoparticles(Pt-NPs) and bimetallic gold–silver nanoparticles(Au Ag-NPs) using aqueous extract of Delonix regia is presented here. Antioxidant activity of biomatrix-loaded metallic nanoparticles is estimated for scavenging of two model radicals i.e., 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and 1,1′-Diphenyl-2-picrylhydrazyl. Broad spectral continuum spanning from visible to ultra-violet region(Pt-NPs: 30 min) and broad high intensity absorption peak around at 500 nm(Au Ag-NPs: 10 min) in two different UV–Visible spectra confirmed the biofabrication. Nanoparticles fabricated with distorted spherical shape and crystalline face-centred-cubic geometry. Strong signal around at 2.10 ke V(pure-phase platinum) and typical X-ray peaks observed at 2.20 and 3 ke V suggested, co-existence and alloying interaction of Au and Ag in Au Ag-NPs. ζ potential(-15.2 mV: Pt-NPs and -13.9 mV: Au Ag-NPs) values suggested surface adsorption of polyphenolic compounds to provide stability. Nanoparticles exhibited pronounced antioxidant activity against free radicals through their electron/hydrogen transfer ability.

The influence of ultraviolet blood irradiation and oxygenation on oxygen free radicals metabolism in rabbits with soman intoxication

Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation (UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were randomly divided into 5 groups: normal control group, intoxication group, routine therapy group. UBIO therapy group and combined therapy group. After 14 d, the concentration of malondiadehyde(MDA) and activity of superoxide dismutase(SOD), glutathionperoxidase(GSH-Px), catalase (CAT) and total antioxidative capacity (T-AOC) in serum were determined respectively. Results: Compared with the normal control group, the concentration of MDA and activity of CAT in the intoxication group were significantly higher (P < 0. 05). but SOD. GSH-Px activity and T-AOC were significantly lower (P<0. 05). After UBIO or combined therapy, serum MDA level was significantly lower in comparison with intoxication group (P<0. 05). but the activity of SOD. GSH-Px, CAT and T-AOC were higher than intoxication group(P<0. 05). Conclusion: There is an obvious oxygen free radical injury in rabbits with a-cute soman intoxication. UBIO can improve the antioxidation ability of rabbits and may be applied to treat acute soman intoxication as adjunctive therapy.

Protective effects of Rad23 protein on ultraviolet damage to HeLa cells

Protein Rad23, a nucleotide excision repair factor, mainly involves in repairing the DNA damage from environment, such as UV light. The function of Rad23 protein involved in DNA damage repair from many environmental factors has been studied extensively, but it is not clear from ultraviolet irradiation. To further investigate the photo-protective function of Rad23 protein on HeLa cells damaged from UV light irradiation, firstly, HeLa cells were irradiated by UV light and incubated with the fusion protein of pCold-Rad23, then the cell viability and apoptosis rate were detected by MTT and Hoechst33342/Pl fluorescent staining, respectively. The results show that the recombinant Rad23 protein can protect the HeLa cells from UV irradiation, and inhibit the apoptosis of HeLa cell by UV irradiation.